PMS1 (NM_001289408) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HNPCC3; hPMS1; MLH2; PMSL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289408, the custom clone sequence may differ by one or more nucleotides ATGAGCTTTGGTATCCTTAAACCTGACTTAAGGATTGTCTTTGTACATAACAAGGCAGTTATTTGGCAGA AAAGCAGAGTATCAGATCACAAGATGGCTCTCATGTCAGTTCTGGGGACTGCTGTTATGAACAATATGGA ATCCTTTCAGTACCACTCTGAAGAATCTCAGATTTATCTCAGTGGATTTCTTCCAAAGTGTGATGCAGAC CACTCTTTCACTAGTCTTTCAACACCAGAAAGAAGTTTCATCTTCATAAACAGTCGACCAGTACATCAAA AAGATATCTTAAAGTTAATCCGACATCATTACAATCTGAAATGCCTAAAGGAATCTACTCGTTTGTATCC TGTTTTCTTTCTGAAAATCGATGTTCCTACAGCTGATGTTGATGTAAATTTAACACCAGATAAAAGCCAA GTATTATTACAAAATAAGGAATCTGTTTTAATTGCTCTTGAAAATCTGATGACGACTTGTTATGGACCAT TACCTAGTACAAATTCTTATGAAAATAATAAAACAGATGTTTCCGCAGCTGACATCGTTCTTAGTAAAAC AGCAGAAACAGATGTGCTTTTTAATAAAGTGGAATCATCTGGAAAGAATTATTCAAATGTTGATACTTCA GTCATTCCATTCCAAAATGATATGCATAATGATGAATCTGGAAAAAACACTGATGATTGTTTAAATCACC AGATAAGTATTGGTGACTTTGGTTATGGTCATTGTAGTAGTGAAATTTCTAACATTGATAAAAACACTAA GAATGCATTTCAGGACATTTCAATGAGTAATGTATCATGGGAGAACTCTCAGACGGAATATAGTAAAACT TGTTTTATAAGTTCCGTTAAGCACACCCAGTCAGAAAATGGCAATAAAGACCATATAGATGAGAGTGGGG AAAATGAGGAAGAAGCAGGTCTTGAAAACTCTTCGGAAATTTCTGCAGATGAGTGGAGCAGGGGAAATAT ACTTAAAAATTCAGTGGGAGAGAATATTGAACCTGTGAAAATTTTAGTGCCTGAAAAAAGTTTACCATGT AAAGTAAGTAATAATAATTATCCAATCCCTGAACAAATGAATCTTAATGAAGATTCATGTAACAAAAAAT CAAATGTAATAGATAATAAATCTGGAAAAGTTACAGCTTATGATTTACTTAGCAATCGAGTAATCAAGAA ACCCATGTCAGCAAGTGCTCTTTTTGTTCAAGATCATCGTCCTCAGTTTCTCATAGAAAATCCTAAGACT AGTTTAGAGGATGCAACACTACAAATTGAAGAACTGTGGAAGACATTGAGTGAAGAGGAAAAACTGAAAT ATGAAGAGAAGGCTACTAAAGACTTGGAACGATACAATAGTCAAATGAAGAGAGCCATTGAACAGGAGTC ACAAATGTCACTAAAAGATGGCAGAAAAAAGATAAAACCCACCAGCGCATGGAATTTGGCCCAGAAGCAC AAGTTAAAAACCTCATTATCTAATCAACCAAAACTTGATGAACTCCTTCAGTCCCAAATTGAAAAAAGAA GGAGTCAAAATATTAAAATGGTACAGATCCCCTTTTCTATGAAAAACTTAAAAATAAATTTTAAGAAACA AAACAAAGTTGACTTAGAAGAGAAGGATGAACCTTGCTTGATCCACAATCTCAGGTTTCCTGATGCATGG CTAATGACATCCAAAACAGAGGTAATGTTATTAAATCCATATAGAGTAGAAGAAGCCCTGCTATTTAAAA GACTTCTTGAGAATCATAAACTTCCTGCAGAGCCACTGGAAAAGCCAATTATGTTAACAGAGAGTCTTTT TAATGGATCTCATTATTTAGACGTTTTATATAAAATGACAGCAGATGACCAAAGATACAGTGGATCAACT TACCTGTCTGATCCTCGTCTTACAGCGAATGGTTTCAAGATAAAATTGATACCAGGAGTTTCAATTACTG AAAATTACTTGGAAATAGAAGGAATGGCTAATTGTCTCCCATTCTATGGAGTAGCAGATTTAAAAGAAAT TCTTAATGCTATATTAAACAGAAATGCAAAGGAAGTTTATGAATGTAGACCTCGCAAAGTGATAAGTTAT TTAGAGGGAGAAGCAGTGCGTCTATCCAGACAATTACCCATGTACTTATCAAAAGAGGACATCCAAGACA TTATCTACAGAATGAAGCACCAGTTTGGAAATGAAATTAAAGAGTGTGTTCATGGTCGCCCATTTTTTCA TCATTTAACCTATCTTCCAGAAACTACATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289408
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289408.1, NP_001276337.1
RefSeq Size	3430 bp
RefSeq ORF	2271 bp
Locus ID	5378
UniProt ID	P54277
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype, which is also known as Lynch syndrome. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) lacks an exon in the 5' coding region, and uses a downstream translation start, compared to variant 1. The encoded protein (isoform d) has a shorter N-terminus, compared to isoform a. Variants 4 and 5 both encode isofo

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