FGD3 (NM_001286993) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZFYVE5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286993, the custom clone sequence may differ by one or more nucleotides ATGGAGTCAGGCAGGGGGTCCTCAACCCCTCCAGGACCCATTGCTGCCCTAGGGATGCCAGACACTGGGC CTGGCAGTTCCTCCCTAGGGAAGCTTCAGGCGCTCCCTGTTGGGCCCAGAGCCCACTGTGGGGACCCTGT CAGCCTGGCTGCAGCAGGGGACGGCTCTCCAGACATAGGCCCCACGGGAGAGCTGAGTGGTAGCTTAAAG ATCCCCAACCGGGACAGCGGGATCGACAGTCCCTCCTCCAGTGTGGCTGGAGAGAACTTTCCCTGCGAGG AGGGCTTGGAGGCTGGCCCAAGCCCCACTGTACTGGGGGCGCACGCAGAGATGGCCCTGGACAGCCAGGT CCCGAAGGTCACCCCCCAGGAGGAGGCGGACAGCGACGTGGGTGAGGAACCTGACTCTGAGAACACCCCC CAGAAGGCTGACAAGGATGCCGGCCTGGCCCAGCACTCTGGCCCCCAGAAGCTTCTCCACATTGCCCAGG AGCTCCTGCACACCGAGGAGACCTATGTGAAGCGGCTGCACCTGCTGGACCAGGTTTTCTGCACCAGGCT GACGGATGCGGGGATCCCTCCAGAAGTCATCATGGGCATATTCTCTAACATCTCCTCCATCCACCGCTTC CACGGGCAGTTCCTGCTGCCGGAGCTGAAGACGCGGATCACGGAGGAGTGGGACACAAACCCACGGCTCG GGGACATCCTGCAGAAGCTGGCCCCATTCCTGAAGATGTACGGCGAGTATGTCAAGAACTTTGACCGAGC CGTAGGGCTGGTGAGCACGTGGACCCAGCGCTCCCCACTGTTTAAAGACGTCGTCCACAGCATCCAGAAG CAGGAGGTATGCGGGAACCTGACGCTGCAGCACCACATGCTGGAGCCCGTGCAGAGGGTCCCCCGGTACG AGCTGCTGCTCAAGGACTATCTGAAGAGGCTCCCGCAGGACGCCCCAGACCGGAAGGATGCGGAGAGGTC CTTGGAGCTCATCTCCACAGCCGCCAACCACTCCAATGCTGCCATTCGGAAAGTGGAGAAAATGCACAAG CTCTTGGAGGTGTACGAGCAGCTGGGTGGGGAAGAAGACATTGTCAACCCGGCCAATGAACTGATCAAGG AGGGCCAAATCCAGAAACTGTCAGCCAAGAACGGCACCCCCCAGGACCGCCACCTCTTCCTGTTCAACAG CATGATCCTTTACTGTGTGCCCAAGCTGCGGCTCATGGGCCAGAAGTTCAGCGTCCGGGAGAAGATGGAC ATCTCAGGCCTCCAGGTGCAGGATATCGTCAAGCCAAACACAGCACATACATTCATCATAACAGGAAGAA AAAGGTCCCTGGAGCTGCAGACGCGGACAGAGGAAGAGAAGAAAGAATGGATTCAGATCATCCAGGCCAC CATCGAGAAGCACAAACAGAACAGCGAAACCTTCAAGGCTTTTGGTGGCGCCTTCAGCCAGGATGAGGAC CCCAGCCTCTCTCCAGACATGCCTATCACGAGCACCAGCCCTGTGGAGCCTGTGGTGACCACCGAAGGCA GTTCGGGTGCAGCAGGGCTCGAGCCCAGAAAACTATCCTCTAAGACCAGACGTGACAAGGAGAAGCAGAG CTGTAAGAGCTGTGGTGAGACCTTCAACTCCATCACCAAGAGGAGGCATCACTGCAAGCTGTGTGGGGCG GTCATCTGTGGGAAGTGCTCCGAGTTCAAGGCCGAGAACAGCCGGCAGAGCCGTGTCTGCAGAGATTGTT TCCTGACACAGCCAGTGGCCCCTGAGAGCACAGAGACACCCACTGCAGACCCCCAGCCCAGCCTGCTCTG CGGCCCCCTGCGGCTGTCAGAGAGCGGTGAGACCTGGAGCGAGGTGTGGGCCGCCATCCCCATGTCAGAT CCCCAGGTGCTGCACCTGCAGGGAGGCAGCCAGGACGGCCGGCTGCCCCGCACCATCCCTCTCCCCAGCT GCAAACTGAGTGTGCCGGACCCTGAGGAGAGGCTGGACTCGGGGCATGTGTGGAAGCTGCAGTGGGCCAA GCAGTCCTGGTACCTGAGCGCCTCCTCCGCAGAGCTGCAGCAGCAGTGGCTGGAAACCCTAAGCACTGCT GCCCATGGGGACACGGCCCAGGACAGCCCGGGGGCCCTGCAGCTTCAGGTCCCTATGGGCGCAGCTGCTC CGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286993
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286993.1, NP_001273922.1
RefSeq Size	3449 bp
RefSeq ORF	2175 bp
Locus ID	89846
UniProt ID	Q5JSP0
Protein Pathways	Regulation of actin cytoskeleton
Gene Summary	Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (b) is 1 aa shorter than isoform a.

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