SIN3B (NM_001297597) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001297597, the custom clone sequence may differ by one or more nucleotides ATGCAGCGTCATTCACGGCATTTCCTCTTGGTGCAGGTACTGAACGACACCTGGGTCTCCTTCCCTTCCT GGTCTGAGGACTCCACGTTCGTCAGCTCCAAGAAGACACCGTACGAGGAGCAGCTTCACCGCTGTGAGGA CGAGCGCTTCGAGTTAGACGTTGTCCTGGAGACGAACCTGGCCACAATCCGTGTGTTGGAAAGTGTGCAG AAGAAGCTGTCTCGGATGGCGCCGGAAGACCAGGAGAAGTTCCGGCTGGACGACTCCCTGGGAGGCACGT CGGAGGTGATCCAGCGCCGTGCCATTTATCGCATCTATGGCGACAAGGCCCCGGAGATCATCGAGAGCCT CAAGAAGAACCCTGTCACCGCTGTCCCCGTTGTCCTGAAAAGACTGAAGGCCAAGGAAGAGGAGTGGCGG GAGGCCCAGCAGGGCTTCAACAAGATCTGGCGGGAGCAGTATGAGAAGGCGTACCTCAAGTCCCTTGACC ACCAGGCTGTGAACTTCAAGCAGAACGACACCAAGGCCCTGCGCTCCAAGAGCTTGCTCAACGAGATCGA GAGCGTCTACGACGAGCACCAGGAGCAGCACTCGGAGGGCCGCAGTGCCCCCTCTAGCGAGCCGCACCTC ATCTTTGTGTACGAGGACCGGCAGATCCTGGAGGACGCAGCAGCGCTCATCAGCTACTACGTGAAGCGGC AGCCGGCCATCCAGAAGGAGGACCAGGGCACCATCCACCAGCTGCTGCACCAGTTCGTGCCCAGCCTCTT CTTCTCTCAGCAGCTGGACCTGGGCGCCTCCGAGGAGTCAGCTGATGAGGACCGGGACAGCCCCCAGGGG CAGACCACAGACCCCAGTGAGCGGAAGAAGCCGGCGCCAGGACCCCACAGTAGCCCCCCAGAGGAGAAGG GGGCCTTCGGGGATGCCCCGGCCACTGAGCAGCCACCCCTGCCGCCCCCAGCCCCGCACAAGCCCCTGGA CGATGTCTACAGCCTATTTTTTGCCAACAACAACTGGTACTTCTTCCTGCGCCTGCACCAGACCCTGTGC TCCAGGCTGCTGAAGATCTACCGCCAGGCGCAGAAGCAGCTTCTGGAGTATCGGACCGAGAAGGAGCGGG AGAAGCTGCTGTGTGAGGGCCGCAGGGAGAAGGGCAGCGACCCCGCCATGGAGCTGCGGCTGAAGCAGCC CAGTGAAGTGGAGCTGGAGGAGTACTACCCGGCCTTCCTGGACATGGTGCGGAGCCTGCTGGAGGGCAGC ATCGACCCCACGCAGTACGAGGACACCCTACGCGAGATGTTCACCATCCATGCCTACGTGGGCTTCACCA TGGACAAGCTGGTGCAGAACATTGCGCGGCAGCTGCACCACCTCGTGAGCGATGACGTCTGCCTGAAGGT GGTGGAGCTCTACCTGAACGAGAAGAAGCGGGGTGCCGCTGGTGGGAACCTGTCCTCCCGCTGCGTCCGC GCTGCTAGGGAGACCAGCTACCAGTGGAAGGCTGAGCGCTGCATGGCCGACGAGAACTGCTTCAAGGTGA TGTTCCTGCAGCGCAAAGGGCAGGTGATCATGACCATCGAGCTCCTGGACACCGAGGAGGCCCAGACGGA GGACCCTGTGGAGGTCCAGCACCTGGCTCGGTACGTGGAGCAGTATGTGGGGACCGAGGGCGCGTCCAGC TCGCCCACTGAGGGCTTCCTCCTGAAACCTGTGTTCCTGCAGAGGAACCTCAAGAAGTTCCGCCGCCGGT GGCAGAGCGAGCAGGCGCGGGCCCTGCGCGGTGAGGCCAGGAGCTCCTGGAAGCGGCTGGTGGGCGTGGA GAGCGCCTGCGACGTGGACTGCCGCTTCAAGCTCAGCACTCACAAGATGGTGTTCATCGTGAACTCCGAG GACTACATGTACCGTCGCGGGACCCTCTGCCGGGCCAAGCAGGTGCAGCCCCTGGTCCTGCTCCGCCACC ACCAGCACTTTGAGGAGTGGCACAGCCGCTGGCTGGAGGACAATGTGACGGTGGAGGCGGCTAGCCTGGT GCAGGACTGGCTGATGGGTGAGGAGGACGAGGACATGGTACCCTGCAAGACGCTGTGTGAGACAGTGCAC GTGCACGGCCTGCCCGTGACCCGCTACCGCGTGCAGTACAGCCGCCGCCCGGCCTCGCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001297597
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297597.1, NP_001284526.1
RefSeq Size	4159 bp
RefSeq ORF	2163 bp
Locus ID	23309
UniProt ID	O75182
Gene Summary	Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct N-terminus, compared to isoform 1.

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