Axotrophin (MARCH7) (NM_001282805) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AXO; AXOT; MARCH-VII; MARCH7; RNF177
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282805, the custom clone sequence may differ by one or more nucleotides ATGGAGTCTAAACCTTCAAGGATTCCAAGAAGAATTTCTGTTCAACCTTCCAGCTCCTTAAGTGCTAGGA TGATGTCTGGAAGCAGAGGAAGTAGTTTAAATGATACCTATCACTCAAGAGACTCTTCATTTAGATTGGA TTCTGAATATCAGTCTACATCAGCATCAGCATCTGCGTCACCATTTCAATCTGCATGGTATAGTGAATCT GAGATAACTCAGGGAGCACGCTCAAGATCGCAGAACCAGCAACGGGATCATGATTCAAAAAGACCTAAAC TTTCCTGTACAAACTGTACTACCTCAGCTGGGAGAAATGTTGGAAATGGTTTAAACACATTATCAGATTC ATCTTGGAGGCATAGTCAAGTTCCTAGATCTTCATCAATGGTACTTGGATCATTTGGAACAGACTTAATG AGAGAGAGGAGAGATTTGGAGAGAAGAACAGATTCCTCTATTAGTAATCTTATGGATTATAGTCACCGAA GTGGTGATTTCACAACTTCATCATATGTTCAAGACAGAGTTCCTTCATATTCACAAGGAGCAAGACCAAA AGAAAACTCAATGAGCACTTTACAGTTGAATACATCATCCACAAACCACCAATTGCCTTCTGAACATCAG ACCATACTAAGTTCTAGGGACTCCAGAAATTCTTTAAGATCAAATTTTTCTTCAAGAGAATCAGAATCTT CCCGAAGCAATACGCAGCCTGGATTTTCTTACAGTTCAAGTAGAGATGAAGCCCCAATCATAAGCAATTC AGAAAGGGTTGTTTCATCTCAAAGACCATTTCAAGAATCTTCTGACAATGAAGGTAGGCGGACAACGAGG AGATTGCTGTCACGCATAGCTTCTAGCATGTCATCTACTTTTTTTTCACGAAGATCTAGTCAGGATTCCT TGAATACAAGATCATTGAATTCTGAAAATTCTTACGTTTCTCCAAGAATCTTGACAGCTTCACAGTCCCG TAGTAATGTACCATCAGCTTCTGAAGTTCCCGATAATAGGGCATCTGAAGCTTCTCAGGGATTTCGATTT CTTAGGCGAAGATGGGGTTTGTCATCTCTTAGCCACAATCATAGCTCTGAGTCAGATTCAGAAAATTTTA ACCAAGAATCTGAAGGTAGAAATACAGGACCATGGTTATCTTCCTCACTTAGAAATAGATGCACACCTTT GTTCTCTAGAAGGAGGCGAGAGGGAAGAGATGAATCTTCAAGGATACCTACCTCTGATACATCATCTAGA TCTCATATTTTTAGAAGAGAATCAAATGAAGTGGTTCACCTTGAAGCACAGAATGATCCTCTTGGAGCTG CTGCCAACAGACCACAAGCATCTGCAGCATCAAGCAGTGCCACAACAGGTGGCTCTACATCAGATTCGGC TCAAGGTGGAAGAAATACAGGAATATCAGGGATTCTTCCTGGTTCCTTATTCCGGTTTGCAGTCCCTCCA GCACTTGGGAGTAATTTGACCGACAATGTCATGATCACAGTAGATATTATTCCTTCAGGTTGGAATTCAG CTGATGGTAAAAGTGATAAAACTAAAAGTGCGCCTTCAAGAGATCCAGAAAGATTGCAGAAAATAAAAGA GAGCCTCCTTTTAGAGGACTCAGAAGAAGAAGAAGGTGACTTATGTAGAATTTGTCAAATGGCAGCTGCA TCATCATCTAATTTGCTGATAGAGCCATGCAAGTGCACAGGAAGTTTGCAGTATGTCCACCAAGACTGTA TGAAAAAGTGGTTACAGGCCAAAATTAACTCTGGTTCTTCATTAGAAGCTGTAACCACCTGTGAACTATG TAAAGAGAAGTTGGAGCTTAACCTGGAGGATTTTGATATTCATGAACTACATAGAGCTCATGCAAATGAA CAAGCTGAGTATGAGTTTATCAGCTCTGGTCTCTACCTAGTGGTGTTATTGCACTTGTGCGAACAAAGCT TTTCTGATATGATGGGAAATACAAATGAACCAAGCACACGTGTCCGATTTATTAACCTTGCAAGAACTCT TCAGGCACATATGGAAGATCTCGAAACTTCAGAGGATGATTCCGAAGAAGACGGAGACCATAACAGGACA TTTGATATTGCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282805
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282805.1, NP_001269734.1
RefSeq Size	3664 bp
RefSeq ORF	2115 bp
Locus ID	64844
UniProt ID	Q9H992
Gene Summary	MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 2 encode isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.