HJURP (NM_001282962) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FAKTS; hFLEG1; URLC9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282962, the custom clone sequence may differ by one or more nucleotides ATGCTGGGTACGCTGCGCGCCATGGAGGGCGAGGACGTGGAAGACGACCAGCTGCTGCAGAAGCTCAGGG CCAGTCGCCGCCGCTTCCAGAGGCGCATGCAGCGGCTGATAGAGAAGTACAACCAGCCCTTCGAGGACAC CCCGGTGGTGCAAATGGCCACGCTGACCTACGAGACGCCACAGGGATTGAGAATTTGGGGTGGAAGACTA ATAAAGGAAAGAAACGAAGGAGAGATCCAGCCTGCAGTGCCTCAAAGCCCTTTGAAAAATGAATTAAGAA GGAAATACTTGACCCAAGTGGATATACTGCTACAAGGTGCAGAGTATTTTGAGTGTGCAGGTAACAGAGC TGGAAGGGATGTACGTGTGACTCCGCTGCCTTCACTGGCCTCACCTGCCGTGCCTGCCCCCGGATACTGC AGTCGTATCTCCAGAAAGAGTCCTGGTGACCCAGCGAAACCAGCTTCATCTCCCAGAGAATGGGATCCTT TGCATCCTTCCTCCACAGACATGGCCTTAGTACCTAGAAATGACAGCCTCTCCCTACAAGAGACCAGTAG CAGCAGCTTCTTAAGCAGCCAGCCCTTTGAAGATGATGACATTTGCAATGTGACCATCAGTGACCTGTAC GCAGGGATGCTGCACTCCATGAGCCGGCTGTTGAGCACAAAGCCATCAAGCATCATCTCCACCAAAACGT TCATCATGCAAAACTGGAACTCCAGGAGGAGGCACAGATATAAGAGCAGGATGAACAAAACATATTGCAA AGGAGCCAGACGTTCTCAGAGGAGCTCCAAGGAGAACTTCATACCCTGCTCTGAGCCTGTGAAAGGGACA GGGGCATTAAGAGATTGCAAGAACGTATTAGATGTTTCTTGCCGTAAGACAGGTTTAAAATTGGAAAAAG CTTTTCTTGAAGTCAACAGACCCCAAATCCATAAGTTAGATCCAAGTTGGAAGGAGCGCAAAGTGACACC CTCGAAGTATTCTTCCTTGATTTACTTCGACTCCAGTGCAACATATAATCTTGATGAGGAAAATAGATTT AGGACATTAAAATGGTTAATTTCTCCTGTAAAAATAGTTTCCAGACCAACAATACGACAGGGCCATGGAG AGAACCGTCAGAGGGAGATTGAAATCCGATTTGATCAGCTTCATCGGGAATATTGCCTGAGTCCCAGGAA CCAGCCTCGCCGGATGTGCCTCCCGGACTCCTGGGCCATGAACATGTACAGAGGGGGTCCTGCGAGTCCT GGTGGCCTTCAGGGCTTAGAAACCCGCAGGCTGAGTTTACCTTCCAGCAAAGCAAAAGCAAAAAGTTTAA GTGAGGCTTTTGAAAACCTAGGCAAAAGATCTCTGGAAGCAGGTAGGTGCCTGCCCAAGAGCGATTCATC TTCATCACTTCCAAAGACCAACCCCACACACAGCGCAACTCGCCCGCAGCAGACATCTGACCTTCACGTT CAGGGAAATAGTTCTGGAATATTTAGAAAGTCAGTGTCACCCAGCAAAACTCTTTCAGTCCCAGATAAAG AAGTGCCAGGCCACGGAAGGAATCGTTACGATGAAATTAAAGAAGAATTTGACAAGCTTCATCAAAAGTA TTGCCTCAAATCTCCTGGGCAGATGACAGTGCCTTTATGTATTGGAGTGTCTACAGATAAAGCAAGTATG GAAGTTCGATATCAAACAGAAGGCTTCTTAGGAAAATTAAATCCAGACCCTCACTTCCAGGGTTTCCAGA AGTTGCCATCATCACCCCTGGGGTGCAGAAAAAGTCTACTGGGCTCAACTGCAATTGAGGCTCCTTCATC TACATGTGTTGCTCGTGCCATCACGAGGGATGGCACGAGGGACCATCAGTTCCCTGCAAAAAGACCCAGG CTATCAGAACCCCAGGGCTCCGGACGCCAGGGCAATTCCCTGGGTGCCTCAGATGGGGTGGACAACACCG TCAGACCGGGAGACCAGGGCAGCTCTTCACAGCCCAACTCAGAAGAGAGAGGAGAGAACACGTCTTACAG GATGGAAGAGAAAAGTGATTTCATGCTAGAAAAATTGGAAACTAAAAGTGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282962
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282962.1, NP_001269891.1
RefSeq Size	3027 bp
RefSeq ORF	2085 bp
Locus ID	55355
UniProt ID	Q8NCD3
Protein Families	Druggable Genome
Gene Summary	Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks two alternate exons that result in the loss of an in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.