IQCE (NM_001287500) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	1700028P05Rik; PAPA7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001287500, the custom clone sequence may differ by one or more nucleotides ATGTTCCTGGGCACCGGGGAGCCGGCCTTGGACACGAAAGCAAAAAGGAAAGCTTTCCACAAACCTCCAC CCACATCGCCAAAGTCACCTTATCTCTCTAAGCCGAGAAAAGTGGCCTCCTGGAGGTCCCTCAGGACGGC AGGGAGCATGCCTCTGGGCGGCCGAGCGTCCCTGACCCCGCAGAAGCTGTGGCTGGGAACCGCAAAGCCA GGAAGTCTGACCCAGGCCCTGAACTCACCCCTCACCTGGGAGCATGCGTGGACTGGCGTCCCCGGCGGCA CTCCTGACTGTCTGACAGACACCTTCAGAGTGAAGAGGCCACATCTCAGGCGCTCTGCCAGCAACGGTCA TGTCCCTGGGACTCCTGTCTACAGAGAAAAAGAAGATATGTATGACGAGATTATTGAGTTAAAGAAGTCA TTGCACGTGCAGAAGAGCGACGTGGACCTGATGAGAACGAAGCTCCGGCGCCTGGAGGAGGAAAACAGCA GGAAGGACCGGCAGATAGAGCAGCTCCTGGATCCCAGCCGCGGCACGGATTTTGTTCGGACTCTGGCAGA GAAAAGGCCCGATGCCAGTTGGGTCATTAACGGGCTGAAGCAGAGGATCCTGAAGCTGGAACAGCAGTGC AAGGAGAAGGACGGCACCATCAGCAAACTCCAGACCGATATGAAGACTACCAACCTGGAAGAGATGCGGA TCGCCATGGAGACATACTACGAGGAGGTGCATCGTCTCCAGACCCTCTTGGCAAGTTCTGAAACCACCGG AAAGAAGCCCCTGGGGGAGAAGAAGACGGGCGCCAAAAGGCAGAAGAAGATGGGCAGTGCCCTCCTGAGC TTGTCCCGGAGTGTCCAGGAGCTCACGGAAGAGAACCAGAGCCTGAAGGAGGACCTGGACCGCGTGCTGA GCACCTCCCCAACCATCTCCAAGACACAGGGTTATGTGGAGTGGAGCAAGCCCCGGCTGCTGAGGCGCAT TGTGGAGCTGGAGAAGAAACTAAGTGTGATGGAGAGCTCAAAATCACACGCCGCAGAGCCAGTCAGATCA CACCCGCCAGCCTGCCTTGCATCCAGCTCTGCGCTGCACAGACAGCCACGAGGGGACCGCAACAAGGACC ACGAGCGTCTCCGAGGGGCTGTGAGAGACCTGAAGGAAGAGCGGACCGCGCTGCAGGAGCAGCTGCTGCA GAGAGATTTGGAGGTGAAGCAGCTCCTGCAGGCGAAGGCCGACCTGGAGAAGGAGCTGGAGTGCGCGAGG GAGGGCGAGGAGGAGAGGAGAGAGCGAGAGGAGGTTTTGAGAGAGGAGATTCAGACACTTACCAGCAAGC TCCAAGAATTGCAAGAAATGAAGAAAGAAGAGAAAGAGGATTGCCCGGAAGTTCCTCATAAGGCCCAAGA GCTCCCAGCTCCCACTCCCAGCAGCAGGCACTGCGAGCAAGACTGGCCGCCGGATTCCAGCGAGGAGGGG CTCCCGCGGCCCCGCTCCCCCTGCTCTGATGGGAGAAGAGACGCCGCGGCCAGAGTCCTGCAGGCCCAGT GGAAGGTGTACAAGCACAAGAAAAAAAAGGCTGTTCTGGATGAGGCGGCTGTGGTGCTTCAGGCAGCTTT CAGGGGACATCTCACGCGGACAAAGCTCTTAGCAAGCAAAGCACATGGCTCAGAGCCACCCAGCGTGCCA GGCCTCCCAGACCAGAGCTCTCCTGTGCCCCGCGTTCCGAGCCCCATCGCCCAGGCCACGGGCAGCCCTG TGCAGGAGGAGGCCATCGTCATCATCCAGTCCGCTCTGCGGGCACACCTGGCCCGGGCCAGGCACAGTGC TACCGGTAAAAGAACCACCACCGCAGCTTCTACCAGGAGGAGATCGGCTTCAGCCACACACGGGGACGCC TCCTCCCCACCCTTCCTCGCAGCTCTTCCTGGTAATTTCATTCATTTGGATTCTGGCACCAGGGCAGCTG GTCTGCTGAGGTCTCAGCCAAAGAGTGGCCACCTCCAGGAAGCCCGGGCTGTGTCGGGACGGAAGGGAGG AGTGTCCCATCTGGAGCTGCCTCTGAACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001287500
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287500.1, NP_001274429.1
RefSeq Size	2466 bp
RefSeq ORF	2061 bp
Locus ID	23288
Gene Summary	Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (By similarity). Required for proper limb morphogenesis (PubMed:28488682).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, and contains an alternate 3' terminal exon and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1. CCDS Note: The coding region was updated to represent an alternative splicing pattern that is more supported by the available transcript and protein data.

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