TAGAP (NM_001278733) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARHGAP47; FKSG15; IDDM21; TAGAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337165 representing NM_001278733. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAAGGCTCTCCCCTGCATCAGATTTTTCTGGGGCTTTGGAGACAGACTTGAAAGCATCGCTATTT GATCAGCCCTTGTCAATTATCTGCGGTGACAGTGACACACTCCCCAGACCCATCCAGGACATTCTCACT ATTCTATGCCTTAAAGGCCCTTCAACGGAAGGGATATTCAGGAGAGCAGCCAACGAGAAAGCCCGTAAG GAGCTGAAGGAGGAGCTCAACTCTGGGGATGCGGTGGATCTGGAGAGGCTCCCCGTGCACCTCCTCGCT GTGGTCTTTAAGGACTTCCTCAGAAGTATCCCCCGGAAGCTACTTTCAAGCGACCTCTTTGAGGAGTGG ATGGGTGCTCTGGAGATGCAGGACGAGGAGGACAGAATCGAGGCCCTGAAACAGGTTGCAGATAAGCTC CCCCGGCCCAACCTCCTGCTACTCAAGCACTTGGTCTATGTGCTGCACCTCATCAGCAAGAACTCTGAG GTGAACAGGATGGACTCCAGCAATCTGGCCATCTGCATTGGACCCAACATGCTCACCCTGGAGAATGAC CAGAGCCTGTCATTTGAAGCCCAGAAGGACCTGAACAACAAGGTGAAGACACTGGTGGAATTCCTCATT GATAACTGCTTTGAAATATTTGGGGAGAACATTCCAGTGCATTCCAGTATCACTTCTGATGACTCCCTG GAGCACACTGACAGTTCAGATGTGTCGACCCTGCAGAATGACTCAGCCTACGACAGCAACGACCCTGAT GTGGAATCCAACAGCAGCAGTGGCATCAGCTCTCCCAGCAGGCAGCCCCAGGTGCCCATGGCCACAGCT GCTGGCTTGGATAGCGCGGGCCCACAGGATGCCCGAGAGGTCAGCCCAGAGCCCATTGTGAGCACCGTG GCCAGGCTGAAAAGCTCCCTCGCACAGCCCGATAGGAGATACTCAGAGCCCAGCATGCCATCCTCCCAG GAGTGCCTCGAGAGCCGGGTGACAAACCAAACACTAACAAAGAGTGAAGGGGACTTCCCCGTGCCCCGG GTAGGCTCTCGTTTGGAAAGTGAGGAGGCTGAAGACCCATTTCCAGAGGAGGTCTTCCCTGCAGTGCAA GGCAAAACCAAGAGGCCGGTGGACCTGAAGATCAAGAACTTGGCCCCGGGTTCGGTGCTCCCGCGGGCA CTGGTTCTCAAAGCCTTCTCCAGCAGCTCGCTGGACGCGTCCTCTGACAGCTCGCCCGTGGCTTCTCCT TCCAGTCCCAAAAGAAATTTCTTCAGCAGACATCAGTCTTTCACCACAAAGACAGAGAAAGGCAAGCCC AGCCGAGAAATTAAAAAGCACTCCATGTCTTTCACCTTTGCCCCTCACAAAAAAGTGCTGACCAAAAAC CTCAGCGCGGGCTCTGGGAAATCGCAAGACTTTACCAGGGACCACGTCCCGAGGGGTGTCAGAAAGGAA AGCCAGCTTGCCGGCCGAATCGTGCAGGAAAATGGGTGTGAAACCCACAACCAAACAGCCCGCGGCTTC TGCCTGAGACCCCACGCCCTCTCGGTGGATGATGTGTTCCAGGGAGCTGACTGGGAGAGGCCTGGAAGC CCACCCTCTTATGAAGAGGCCATGCAGGGCCCGGCAGCCAGACTAGTGGCCTCCGAGAGCCAGACCGTG GGGAGCATGACGGTGGGGAGCATGAGGGCGAGGATGCTGGAGGCGCACTGCCTCCTACCCCCTCTTCCA CCTGCTCACCACGTAGAGGACTCAAGACACAGGGGCAGCAAAGAGCCACTCCCTGGCCACGGACTCTCT CCCCTGCCTGAGCGATGGAAACAGAGCAGAACTGTCCATGCTTCTGGGGACTCTCTGGGGCACGTGTCT GGCCCAGGGAGACCTGAGCTCCTCCCGCTGAGGACCGTCTCCGAGTCCGTGCAGAGGAATAAGCGGGAC TGTCTCGTGCGACGATGTAGCCAGCCGGTCTTTGAGGCTGACCAATTCCAATATGCCAAAGAATCGTAT ATTTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001278733
Insert Size	2007 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278733.1
RefSeq Size	3516 bp
RefSeq ORF	2007 bp
Locus ID	117289
UniProt ID	Q8N103
MW	73.5 kDa
Gene Summary	This gene encodes a member of the Rho GTPase-activator protein superfamily. The encoded protein may function as a Rho GTPase-activating protein. Alterations in this gene may be associated with several diseases, including rheumatoid arthritis, celiac disease, and multiple sclerosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (4) uses an alternate 5'-most exon, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 2. The encoded isoform (d) has a shorter N-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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