TAGAP (NM_001278733) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARHGAP47; FKSG15; IDDM21; TAGAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239271 representing NM_001278733 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGGCTCTCCCCTGCATCAGATTTTTCTGGGGCTTTGGAGACAGACTTGAAAGCATCGCTATTTG ATCAGCCCTTGTCAATTATCTGCGGTGACAGTGACACACTCCCCAGACCCATCCAGGACATTCTCACTAT TCTATGCCTTAAAGGCCCTTCAACGGAAGGGATATTCAGGAGAGCAGCCAACGAGAAAGCCCGTAAGGAG CTGAAGGAGGAGCTCAACTCTGGGGATGCGGTGGATCTGGAGAGGCTCCCCGTGCACCTCCTCGCTGTGG TCTTTAAGGACTTCCTCAGAAGTATCCCCCGGAAGCTACTTTCAAGCGACCTCTTTGAGGAGTGGATGGG TGCTCTGGAGATGCAGGACGAGGAGGACAGAATCGAGGCCCTGAAACAGGTTGCAGATAAGCTCCCCCGG CCCAACCTCCTGCTACTCAAGCACTTGGTCTATGTGCTGCACCTCATCAGCAAGAACTCTGAGGTGAACA GGATGGACTCCAGCAATCTGGCCATCTGCATTGGACCCAACATGCTCACCCTGGAGAATGACCAGAGCCT GTCATTTGAAGCCCAGAAGGACCTGAACAACAAGGTGAAGACACTGGTGGAATTCCTCATTGATAACTGC TTTGAAATATTTGGGGAGAACATTCCAGTGCATTCCAGTATCACTTCTGATGACTCCCTGGAGCACACTG ACAGTTCAGATGTGTCGACCCTGCAGAATGACTCAGCCTACGACAGCAACGACCCTGATGTGGAATCCAA CAGCAGCAGTGGCATCAGCTCTCCCAGCAGGCAGCCCCAGGTGCCCATGGCCACAGCTGCTGGCTTGGAT AGCGCGGGCCCACAGGATGCCCGAGAGGTCAGCCCAGAGCCCATTGTGAGCACCGTGGCCAGGCTGAAAA GCTCCCTCGCACAGCCCGATAGGAGATACTCAGAGCCCAGCATGCCATCCTCCCAGGAGTGCCTCGAGAG CCGGGTGACAAACCAAACACTAACAAAGAGTGAAGGGGACTTCCCCGTGCCCCGGGTAGGCTCTCGTTTG GAAAGTGAGGAGGCTGAAGACCCATTTCCAGAGGAGGTCTTCCCTGCAGTGCAAGGCAAAACCAAGAGGC CGGTGGACCTGAAGATCAAGAACTTGGCCCCGGGTTCGGTGCTCCCGCGGGCACTGGTTCTCAAAGCCTT CTCCAGCAGCTCGCTGGACGCGTCCTCTGACAGCTCGCCCGTGGCTTCTCCTTCCAGTCCCAAAAGAAAT TTCTTCAGCAGACATCAGTCTTTCACCACAAAGACAGAGAAAGGCAAGCCCAGCCGAGAAATTAAAAAGC ACTCCATGTCTTTCACCTTTGCCCCTCACAAAAAAGTGCTGACCAAAAACCTCAGCGCGGGCTCTGGGAA ATCGCAAGACTTTACCAGGGACCACGTCCCGAGGGGTGTCAGAAAGGAAAGCCAGCTTGCCGGCCGAATC GTGCAGGAAAATGGGTGTGAAACCCACAACCAAACAGCCCGCGGCTTCTGCCTGAGACCCCACGCCCTCT CGGTGGATGATGTGTTCCAGGGAGCTGACTGGGAGAGGCCTGGAAGCCCACCCTCTTATGAAGAGGCCAT GCAGGGCCCGGCAGCCAGACTAGTGGCCTCCGAGAGCCAGACCGTGGGGAGCATGACGGTGGGGAGCATG AGGGCGAGGATGCTGGAGGCGCACTGCCTCCTACCCCCTCTTCCACCTGCTCACCACGTAGAGGACTCAA GACACAGGGGCAGCAAAGAGCCACTCCCTGGCCACGGACTCTCTCCCCTGCCTGAGCGATGGAAACAGAG CAGAACTGTCCATGCTTCTGGGGACTCTCTGGGGCACGTGTCTGGCCCAGGGAGACCTGAGCTCCTCCCG CTGAGGACCGTCTCCGAGTCCGTGCAGAGGAATAAGCGGGACTGTCTCGTGCGACGATGTAGCCAGCCGG TCTTTGAGGCTGACCAATTCCAATATGCCAAAGAATCGTATATT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239271 representing NM_001278733 Red=Cloning site Green=Tags(s) MRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKE LKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPR PNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVKTLVEFLIDNC FEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVESNSSSGISSPSRQPQVPMATAAGLD SAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECLESRVTNQTLTKSEGDFPVPRVGSRL ESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLKAFSSSSLDASSDSSPVASPSSPKRN FFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGSGKSQDFTRDHVPRGVRKESQLAGRI VQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEEAMQGPAARLVASESQTVGSMTVGSM RARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWKQSRTVHASGDSLGHVSGPGRPELLP LRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278733
ORF Size	2004 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278733.2
RefSeq Size	3516 bp
RefSeq ORF	2007 bp
Locus ID	117289
UniProt ID	Q8N103
MW	74 kDa
Gene Summary	This gene encodes a member of the Rho GTPase-activator protein superfamily. The encoded protein may function as a Rho GTPase-activating protein. Alterations in this gene may be associated with several diseases, including rheumatoid arthritis, celiac disease, and multiple sclerosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]

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