GCP4 (TUBGCP4) (NM_001286414) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	76P; GCP-4; GCP4; Grip76; MCCRP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337161 representing NM_001286414. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCCACGAACTGCTCTTGGCTCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGT GGCCTGCAGGTATCGCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGC CGGCTCGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTGCAACAGCAG GATCACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGCGGGCCTTCTGCACAGGG CTGGATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGAACAAGAGTTCCTGGGTGATCCC CATCTCTCCATATCACATGTCAACTACTTCCTAGACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTT GTAGTAGAACAAATTAAAAGTCAAAAGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGC TGTGGGGGGTTGCCTCCTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTAT AAACAGCTCTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAACAG GGGCCATCTTCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGCATTGGGGGACTG ACAGGAAAACAACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAAGAGAACATGCTGGCACCATCT CTGAAGCAGTTTTCCCTACGAGTGGAGATTTTGCCATCCTACATTCCAGTGAGGGTTGCTGAAAAAATC CTATTTGTTGGAGAATCTGTCCAGATGTTTGAGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATT TTGAAAAACCAGGAAGACACTTTTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTG GTGGACTTTGAACAGGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAGTTGATGGTA GAAGAATCCGATTTACTGGGTCAGCTGAAGATCATTAAAGACTTTTACCTTCTGGGACGTGGAGAACTG TTTCAGGCCTTCATTGACACAGCTCAACACATGTTGAAAACACCACCCACTGCAGTAACTGAGCATGAT GTGAATGTGGCCTTTCAACAGTCAGCACACAAGGTATTGCTAGATGATGACAACCTTCTCCCTCTGTTG CACTTGACAATCGAGTATCACGGAAAGGAGCACAAAGCAGATGCTACTCAGGCAAGAGAAGGGCCTTCT CGGGAAACTTCTCCCCGGGAAGCCCCTGCATCTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAG TGGCCACTACATATTCTCTTCACCCCAGCTGTCCTGGAAAAGTACAATGTTGTTTTTAAGTACTTACTG AGTGTGCGCCGGGTGCAAGCTGAGCTGCAGCACTGCTGGGCCCTACAAATGCAGCGCAAGCACCTCAAG TCGAACCAGACTGATGCAATCAAGTGGCGCCTAAGAAATCACATGGCATTTTTGGTGGATAATCTTCAG TACTATCTCCAGGTAGATGTGTTGGAGTCTCAGTTCTCCCAGCTGCTTCATCAGATCAATTCTACCCGA GACTTTGAAAGCATCCGATTGGCTCATGACCACTTCCTGAGCAATTTGCTGGCTCAATCCTTTATCCTA TTGAAACCTGTGTTTCACTGCCTGAATGAAATCCTAGATCTCTGTCACAGTTTTTGTTCGCTGGTCAGT CAGAACCTAGGCCCACTGGATGAGCGTGGAGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCCGC CAGTCTTCACTCCTGTTCAAGATTCTCTCCAGTGTTCGGAATCATCAGATCAACTCAGATTTGGCTCAA CTACTGTTACGACTAGATTATAACAAATACTATACCCAGGCTGGTGGAACTCTGGGCAGTTTCGGGATG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286414
Insert Size	2004 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286414.2
RefSeq Size	4197 bp
RefSeq ORF	2004 bp
Locus ID	27229
UniProt ID	Q9UGJ1
MW	76.1 kDa
Gene Summary	This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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