Calpastatin (CAST) (NM_001284212) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BS-17; PLACK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337157 representing NM_001284212. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCCAAAA AGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCA GCTGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGT GGAGAGAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCA TTAACCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCT TTGGATGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGA CCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACAATTCCT CCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACCC ATAGGGCCAGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGA AAGAAAACTGAAAAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCT GCTCCACCCCAAGAGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTG TCGGCTTCACTGGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAG GCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGA TTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGG AGTGAATCAGAACTCATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCT AAGCCAACTGAAAAGACAGAAGAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGG ACCTCCATGTGTAGTATACAGTCAGCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGAT GCTGTAGAAGCCTTGGCTGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATG GATAAAGTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCT CCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAA CAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAAT GCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCA GCTTCAACGACCCAAGCTGGAGCCCCACCCCGTGATACCTCGAGTGACAAAGACCTCGATGATGCCTTG GATAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAA GTAAAGGAAAAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAA TACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGAT TCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCC ACTACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCT AAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284212
Insert Size	2001 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284212.2
RefSeq Size	2392 bp
RefSeq ORF	2001 bp
Locus ID	831
UniProt ID	P20810
MW	71.8 kDa
Gene Summary	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

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