ITGB6 (NM_001282390) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AI1H
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337097 representing NM_001282390. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCTCTCCGCCTCCATGGATGACGACCTCAACACAATAAAGGAGCTGGGCTCCCGGCTTTCCAAA GAGATGTCTAAATTAACCAGCAACTTTAGACTGGGCTTCGGATCTTTTGTGGAAAAACCTGTATCCCCT TTTGTGAAAACAACACCAGAAGAAATTGCCAACCCTTGCAGTAGTATTCCATACTTCTGTTTACCTACA TTTGGATTCAAGCACATTTTGCCATTGACAAATGATGCTGAAAGATTCAATGAAATTGTGAAGAATCAG AAAATTTCTGCTAATATTGACACACCCGAAGGTGGATTTGATGCAATTATGCAAGCTGCTGTGTGTAAG GAAAAAATTGGCTGGCGGAATGACTCCCTCCACCTCCTGGTCTTTGTGAGTGATGCTGATTCTCATTTT GGAATGGACAGCAAACTAGCAGGCATCGTCATTCCTAATGACGGGCTCTGTCACTTGGACAGCAAGAAT GAATACTCCATGTCAACTGTCTTGGAATATCCAACAATTGGACAACTCATTGATAAACTGGTACAAAAC AACGTGTTATTGATCTTCGCTGTAACCCAAGAACAAGTTCATTTATATGAGAATTACGCAAAACTTATT CCTGGAGCTACAGTAGGTCTACTTCAGAAGGACTCCGGAAACATTCTCCAGCTGATCATCTCAGCTTAT GAAGAACTGCGGTCTGAGGTGGAACTGGAAGTATTAGGAGACACTGAAGGACTCAACTTGTCATTTACA GCCATCTGTAACAACGGTACCCTCTTCCAACACCAAAAGAAATGCTCTCACATGAAAGTGGGAGACACA GCTTCCTTCAGCGTGACTGTGAATATCCCACACTGCGAGAGAAGAAGCAGGCACATTATCATAAAGCCT GTGGGGCTGGGGGATGCCCTGGAATTACTTGTCAGCCCAGAATGCAACTGCGACTGTCAGAAAGAAGTG GAAGTGAACAGCTCCAAATGTCACCACGGGAACGGCTCTTTCCAGTGTGGGGTGTGTGCCTGCCACCCT GGCCACATGGGGCCTCGCTGTGAGTGTGGCGAGGACATGCTGAGCACAGATTCCTGCAAGGAGGCCCCA GATCATCCCTCCTGCAGCGGAAGGGGTGACTGCTACTGTGGGCAGTGTATCTGCCACTTGTCTCCCTAT GGAAACATTTATGGGCCTTATTGCCAGTGTGACAATTTCTCCTGCGTGAGACACAAAGGGCTGCTCTGC GGAGGTAACGGCGACTGTGACTGTGGTGAATGTGTGTGCAGGAGCGGCTGGACTGGCGAGTACTGCAAC TGCACCACCAGCACGGACTCCTGCGTCTCTGAAGATGGAGTGCTCTGCAGCGGGCGCGGGGACTGTGTT TGTGGCAAGTGTGTTTGCACAAACCCTGGAGCCTCAGGACCAACCTGTGAACGATGTCCTACCTGTGGT GACCCCTGTAACTCTAAACGGAGCTGCATTGAGTGCCACCTGTCAGCAGCTGGCCAAGCCCGAGAAGAA TGTGTGGACAAGTGCAAACTAGCTGGTGCGACCATCAGTGAAGAAGAAGATTTCTCAAAGGATGGTTCT GTTTCCTGCTCTCTGCAAGGAGAAAATGAATGTCTTATTACATTCCTAATAACTACAGATAATGAGGGG AAAACCATCATTCACAGCATCAATGAAAAAGATTGTCCGAAGCCTCCAAACATTCCCATGATCATGTTA GGGGTTTCCCTGGCTATTCTTCTCATCGGGGTTGTCCTACTGTGCATCTGGAAGCTACTGGTGTCATTT CATGATCGTAAAGAAGTTGCCAAATTTGAAGCAGAACGATCAAAAGCCAAGTGGCAAACGGGAACCAAT CCACTCTACAGAGGATCCACAAGTACTTTTAAAAATGTAACTTATAAACACAGGGAAAAACAAAAGGTA GACCTTTCCACAGATTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282390
Insert Size	1953 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282390.1
RefSeq Size	4440 bp
RefSeq ORF	1953 bp
Locus ID	3694
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
MW	70.9 kDa
Gene Summary	This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms a dimer with an alpha v chain and this heterodimer can bind to ligands like fibronectin and transforming growth factor beta 1. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (7) lacks an alternate 5' exon and uses an alternate 5' splice site, compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG resulting in a shorter isoform (f) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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