SUV420h1 (KMT5B) (NM_001300908) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGI-85; CGI85; MRD51; SUV420H1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337085 representing NM_001300908. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTACTTAGACATGGAGAAAACGACTTCAGTGTCATGTACTCCACAAGGAAAAACTGTGCTCAACTC TGGCTGGGTCCTGCTGCGTTTATAAACCATGATTGCAGACCTAATTGTAAGTTTGTGTCAACTGGTCGA GATACAGCATGTGTGAAGGCTCTAAGAGACATTGAACCTGGAGAAGAAATTTCTTGTTATTATGGAGAT GGGTTCTTTGGAGAAAATAATGAGTTCTGCGAGTGTTACACTTGCGAAAGACGGGGCACTGGTGCTTTT AAATCCAGAGTGGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATATGGACTCAGAGAAACAGAT AAACGTTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAATTCAGACAGTCAATCTGTCAGCTCT AACACTGATGCAGATACCACTCAGGAAAAAAACAATGCAACTTCTAACCGAAAATCTTCAGTTGGCGTA AAAAAGAATAGCAAGAGCAGAACGTTAACGAGGCAATCTATGTCAAGAATTCCAGCTTCTTCCAACTCT ACCTCATCTAAGCTAACTCATATAAATAATTCCAGGGTACCAAAGAAACTGAAGAAGCCTGCAAAGCCT TTACTTTCAAAGATAAAATTGAGAAATCATTGCAAGCGGCTGGAGCAAAAGAATGCTTCAAGAAAACTC GAAATGGGAAACTTAGTACTGAAAGAGCCTAAAGTAGTTCTGTATAAAAATTTGCCCATTAAAAAAGAT AAGGAGCCAGAGGGACCAGCCCAAGCCGCAGTTGCCAGCGGGTGCTTGACTAGACACGCGGCGAGAGAA CACAGACAGAATCCTGTGAGAGGTGCTCATTCGCAGGGGGAGAGCTCGCCCTGCACCTACATAACTCGG CGGTCAGTGAGGACAAGAACAAATCTGAAGGAGGCCTCTGACATCAAGCTTGAACCAAATACGTTGAAT GGCTATAAAAGCAGTGTGACGGAACCTTGCCCCGACAGTGGTGAACAGCTGCAGCCAGCTCCTGTGCTG CAGGAGGAAGAACTGGCTCATGAGACTGCACAAAAAGGGGAGGCAAAGTGTCATAAGAGTGACACAGGC ATGTCCAAAAAGAAGTCACGACAAGGAAAACTTGTGAAACAGTTTGCAAAAATAGAGGAATCTACTCCA GTGCACGATTCTCCTGGAAAAGACGACGCGGTACCAGATTTGATGGGTCCCCATTCTGACCAGGGTGAG CACAGTGGCACTGTGGGCGTGCCTGTGAGCTACACAGACTGTGCTCCTTCACCCGTCGGTTGTTCAGTT GTGACATCAGATAGCTTCAAAACAAAAGACAGCTTTAGAACTGCAAAAAGTAAAAAGAAGAGGCGAATC ACAAGGTATGATGCACAGTTAATCCTAGAAAATAACTCTGGGATTCCCAAATTGACTCTTCGTAGGCGT CATGATAGCAGCAGCAAAACAAATGACCAAGAGAATGATGGAATGAACTCTTCCAAAATAAGCATCAAG TTAAGCAAAGACCATGACAACGATAACAATCTCTATGTAGCAAAGCTTAATAATGGATTTAACTCAGGA TCAGGCAGTAGTTCTACAAAATTAAAAATCCAGCTAAAACGAGATGAGGAAAATAGGGGGTCTTATACA GAGGGGCTTCATGAAAATGGGGTGTGCTGCAGTGATCCTCTTTCTCTCTTGGAGTCTCGAATGGAGGTG GATGACTATAGTCAGTATGAGGAAGAAAGTACAGATGATTCCTCCTCTTCTGAGGGCGATGAAGAGGAG GATGACTATGATGATGACTTTGAAGACGATTTTATTCCTCTTCCTCCAGCTAAGCGCTTGAGGTTAATA GTTGGAAAAGACTCTATAGATATTGACATTTCTTCAAGGAGAAGAGAAGATCAGTCTTTAAGGCTTAAT GCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300908
Insert Size	1938 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300908.1
RefSeq Size	5802 bp
RefSeq ORF	1938 bp
Locus ID	51111
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation
MW	71.6 kDa
Gene Summary	This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (4) uses an alternate first exon and an alternate splice junction at the 5' end of an exon, and uses a downstream start codon compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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