Znf206 (ZSCAN10) (NM_001282416) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZFP206; ZNF206
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337081 representing NM_001282416. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTCCAGTATCAGGAGGACATGGGGCCACGGGCGTCCCTGAGCCGGCTCCGGGAGCTCTGCGGCCA CTGGCTGCGGCCGGCTCTGCACACCAAGAAACAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTGAG TGTGCTGCCTCCGCACCTCCTGGGCCGCCTGCAGGGGCAGCCGCTCAGGGATGGGGAGGAGGTGGTGCT GCTGCTCGAGGGCATCCACCGGGAGCCCAGCCACGCGGGGCCGCTGGTCCCCAGGGCCCCTCACCATGG CCAGAGGAGAGTTCCCGAGATCAGGAGCTGGCGGCTGTGCTGGAGTGCCTGACCTTTGAGGATGTGCCA GAGAATAAGGCGTGGCCTGCACACCCCCTGGGATTCGGAAGCAGAACCCCAGACAAGGAGGAATTTAAA CAAGAAGAGCCCAAAGGGGCTGCCTGGCCCACTCCCATCTTAGCAGAGTCCCAGGCAGATAGTCCTGGG GTGCCGGGAGAGCCTTGCGCCCAGTCGCTCGGACGGGGCGCTGCGGCTAGCGGCCCTGGTGAAGATGGG TCCCTTCTTGGCAGCAGTGAAATTTTGGAGGTCAAAGTGGCTGAGGGCGTCCCCGAGCCCAATCCGGAG TTGCAGTTCATCTGCGCGGACTGCGGGGTGAGCTTCCCGCAGCTGTCTCGCCTGAAGGCGCACCAGCTG CGCTCGCACCCGGCTGGGCGCTCCTTCCTGTGCCTTTGCTGCGGGAAGAGCTTCGGCCGCAGCTCCATT CTCAAGCTGCACATGCGCACTCACACGGACGAGCGGCCGCACGCCTGCCACCTGTGCGGCCACCGCTTC CGCCAGAGCTCGCACCTGAGCAAGCACCTGCTGACCCACTCCTCCGAACCCGCCTTCCTGTGCGCAGAG TGCGGCCGCGGCTTCCAGCGCCGCGCCAGCCTTGTGCAGCACCTGCTGGCGCACGCCCAGGACCAGAAG CCGCCCTGCGCTCCTGAGAGTAAGGCCGAAGCGCCGCCACTGACCGATGTCCTGTGCTCCCACTGCGGC CAGAGCTTCCAGCGCCGCTCCAGCCTCAAGCGCCACCTGCGGATCCACGCCAGGGACAAGGACCGCCGG TCCTCCGAAGGCTCCGGCAGCCGCCGCCGGGACTCCGACCGGAGGCCCTTCGTGTGCAGCGACTGCGGC AAGGCCTTCCGGCGCAGCGAGCACCTGGTGGCCCACCGGAGGGTGCACACGGGCGAGCGGCCCTTCTCC TGCCAGGCTTGCGGCCGCAGCTTCACGCAGAGCTCGCAGCTGGTCAGCCACCAACGGGTGCACACGGGC GAGAAGCCCTACGCCTGTCCGCAGTGCGGGAAGCGCTTTGTGCGCCGGGCCAGCCTTGCCCGCCACCTG CTGACCCACGGTGGCCCTCGGCCCCACCACTGCACCCAGTGCGGGAAGAGTTTCGGCCAGACCCAGGAT CTGGCCCGCCACCAGCGCAGCCACACGGGCGAGAAGCCCTGCCGCTGCAGCGAGTGCGGTGAGGGCTTC AGCCAGAGCGCCCACCTGGCGCGCCACCAGCGCATCCACACAGGGGAGAAGCCCCACGCCTGCGACACC TGCGGCCACCGTTTCCGCAATAGCTCCAACCTGGCCCGCCATCGCCGCAGCCACACGGGCGAGCGGCCC TACAGCTGTCAGACGTGCGGTCGCAGCTTCCGGCGCAACGCGCATCTGCGGCGGCACCTGGCTACCCAT GCGGAGCCCGGGCAGGAGCAGGCCGAGCCCCCGCAGGAGTGCGTGGAGTGCGGGAAGAGCTTCAGCCGC AGCTGCAATCTGCTGCGACACCTGCTGGTGCACACGGGCGCCAGGCCCTACTCCTGCACGCAGTGTGGC CGCAGCTTCAGCCGCAACTCCCACCTGCTGCGCCACCTGCGCACCCACGCCCGCGAGACGCTGTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282416
Insert Size	1932 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282416.1
RefSeq Size	2476 bp
RefSeq ORF	1932 bp
Locus ID	84891
UniProt ID	Q96SZ4
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
MW	69.7 kDa
Gene Summary	Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency. Can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an exon in the 5' coding region which results in the use of an alternate start codon compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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