Axotrophin (MARCH7) (NM_001282806) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AXO; AXOT; MARCH-VII; MARCH7; RNF177
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337060 representing NM_001282806. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTCTAAACCTTCAAGGATTCCAAGAAGAATTTCTGTTCAACCTTCCAGCTCCTTAAGTGCTAGG ATGATGTCTGGAAGCAGAGGAAGTAGTTTAAATGATACCTATCACTCAAGAGACTCTTCATTTAGATTG GATTCTGAATATCAGTCTACATCAGCATCAGCATCTGCGTCACCATTTCAATCTGCATGGTATAGTGAA TCTGAGATAACTCAGGGAGCACGCTCAAGATCGCAGAACCAGCAACGGGATCATGATTCAAAAAGACCT AAACTTTCCTGTACAAACTGTACTACCTCAGCTGGGAGAAATGTTGGAAATGGTTTAAACACATTATCA GATGTTCAAGACAGAGTTCCTTCATATTCACAAGGAGCAAGACCAAAAGAAAACTCAATGAGCACTTTA CAGTTGAATACATCATCCACAAACCACCAATTGCCTTCTGAACATCAGACCATACTAAGTTCTAGGGAC TCCAGAAATTCTTTAAGATCAAATTTTTCTTCAAGAGAATCAGAATCTTCCCGAAGCAATACGCAGCCT GGATTTTCTTACAGTTCAAGTAGAGATGAAGCCCCAATCATAAGCAATTCAGAAAGGGTTGTTTCATCT CAAAGACCATTTCAAGAATCTTCTGACAATGAAGGTAGGCGGACAACGAGGAGATTGCTGTCACGCATA GCTTCTAGCATGTCATCTACTTTTTTTTCACGAAGATCTAGTCAGGATTCCTTGAATACAAGATCATTG AATTCTGAAAATTCTTACGTTTCTCCAAGAATCTTGACAGCTTCACAGTCCCGTAGTAATGTACCATCA GCTTCTGAAGTTCCCGATAATAGGGCATCTGAAGCTTCTCAGGGATTTCGATTTCTTAGGCGAAGATGG GGTTTGTCATCTCTTAGCCACAATCATAGCTCTGAGTCAGATTCAGAAAATTTTAACCAAGAATCTGAA GGTAGAAATACAGGACCATGGTTATCTTCCTCACTTAGAAATAGATGCACACCTTTGTTCTCTAGAAGG AGGCGAGAGGGAAGAGATGAATCTTCAAGGATACCTACCTCTGATACATCATCTAGATCTCATATTTTT AGAAGAGAATCAAATGAAGTGGTTCACCTTGAAGCACAGAATGATCCTCTTGGAGCTGCTGCCAACAGA CCACAAGCATCTGCAGCATCAAGCAGTGCCACAACAGGTGGCTCTACATCAGATTCGGCTCAAGGTGGA AGAAATACAGGAATATCAGGGATTCTTCCTGGTTCCTTATTCCGGTTTGCAGTCCCTCCAGCACTTGGG AGTAATTTGACCGACAATGTCATGATCACAGTAGATATTATTCCTTCAGGTTGGAATTCAGCTGATGGT AAAAGTGATAAAACTAAAAGTGCGCCTTCAAGAGATCCAGAAAGATTGCAGAAAATAAAAGAGAGCCTC CTTTTAGAGGACTCAGAAGAAGAAGAAGGTGACTTATGTAGAATTTGTCAAATGGCAGCTGCATCATCA TCTAATTTGCTGATAGAGCCATGCAAGTGCACAGGAAGTTTGCAGTATGTCCACCAAGACTGTATGAAA AAGTGGTTACAGGCCAAAATTAACTCTGGTTCTTCATTAGAAGCTGTAACCACCTGTGAACTATGTAAA GAGAAGTTGGAGCTTAACCTGGAGGATTTTGATATTCATGAACTACATAGAGCTCATGCAAATGAACAA GCTGAGTATGAGTTTATCAGCTCTGGTCTCTACCTAGTGGTGTTATTGCACTTGTGCGAACAAAGCTTT TCTGATATGATGGGAAATACAAATGAACCAAGCACACGTGTCCGACTTCAGAGGATGATTCCGAAGAAG ACGGAGACCATAACAGGACATTTGATATTGCCTAACTTCATATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282806
Insert Size	1908 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282806.1
RefSeq Size	3319 bp
RefSeq ORF	1908 bp
Locus ID	64844
UniProt ID	Q9H992
MW	70.1 kDa
Gene Summary	MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (3) differs in the 5' UTR, lacks an alternate in-frame exon in the 5' coding region, and lacks an exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a.

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