p66 alpha (GATAD2A) (NM_001300946) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	p66alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337056 representing NM_001300946. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGAAGAAGCATGCCGAACACGGAGTCAGAAACGAGCGCTTGAACGGGACCCAACAGAGGACGAT GTGGAGAGCAAGAAAATAAAAATGGAGAGAGGATTGTTGGCTTCAGATTTAAACACTGACGGAGACATG AGGGTGACACCTGAGCCGGGAGCAGGTCCAACCCAAGGATTGCTGAGGGCAACAGAGGCCACGGCCATG GCCATGGGCAGAGGCGAAGGGCTGGTGGGCGATGGGCCCGTGGACATGCGCACCTCACACAGTGACATG AAGTCCGAGAGGAGACCCCCCTCACCTGACGTGATTGTGCTCTCCGACAACGAGCAGCCCTCGAGCCCG AGAGTGAATGGGCTGACCACGGTGGCCTTGAAGGAGACTAGCACCGAGGCCCTCATGAAAAGCAGTCCT GAAGAACGAGAAAGGATGATCAAGCAGCTGAAGGAAGAATTGAGGTTAGAAGAAGCAAAACTCGTGTTG TTGAAAAAGTTGCGGCAGAGTCAAATACAAAAGGAAGCCACCGCCCAGAAGCCCACAGGTTCTGTTGGG AGCACCGTGACCACCCCTCCCCCGCTTGTTCGGGGCACTCAGAACATTCCTGCTGGCAAGCCATCACTC CAGACCTCTTCAGCTCGGATGCCCGGCAGTGTCATACCCCCGCCCCTGGTCCGAGGTGGGCAGCAGGCG TCCTCGAAGCTGGGGCCACAGGCGAGCTCACAGGTCGTCATGCCCCCACTCGTCAGGGGGGCTCAGCAA ATCCACAGCATTAGGCAACATTCCAGCACAGGGCCACCGCCCCTCCTCCTGGCCCCCCGGGCGTCGGTG CCCAGTGTGCAGATTCAGGGACAGAGGATCATCCAGCAGGGCCTCATCCGCGTCGCCAATGTTCCCAAC ACCAGCCTGCTCGTCAACATCCCACAGCCCACCCCAGCATCACTGAAGGGGACAACAGCCACCTCCGCT CAGGCCAACTCCACCCCCACTAGTGTGGCCTCTGTGGTCACCTCTGCCGAGTCTCCAGCAAGCCGACAG GCGGCCGCCAAGCTGGCGCTGCGCAAACAGCTGGAGAAGACGCTACTCGAGATCCCCCCACCCAAGCCC CCAGCCCCAGAGATGAACTTCCTGCCCAGCGCCGCCAACAACGAGTTCATCTACCTGGTCGGCCTGGAG GAGGTGGTGCAGAACCTACTGGAGACACAAGCAGGCAGGATGTCGGCCGCCACTGTGCTGTCCCGGGAG CCCTACATGTGTGCACAGTGCAAGACGGACTTCACGTGCCGCTGGCGGGAGGAGAAGAGCGGCGCCATC ATGTGTGAGAACTGCATGACAACCAACCAGAAGAAGGCGCTCAAGGTGGAGCACACCAGCCGGCTGAAG GCCGCCTTTGTGAAGGCGCTGCAGCAGGAACAGGAGATTGAGCAGCGGCTCCTGCAGCAGGGCACGGCC CCTGCACAGGCCAAGGCCGAGCCCACCGCTGCCCCACACCCCGTGCTGAAGCAGGTCATAAAACCCCGG CGTAAGTTGGCGTTCCGCTCAGGAGAGGCCCGCGACTGGAGTAACGGGGCTGTGCTACAGGCCTCCAGC CAGCTGTCCCGGGGTTCGGCCACGACGCCCCGAGGTGTCCTGCACACGTTCAGTCCGTCACCCAAACTG CAGAACTCAGCCTCGGCCACAGCCCTGGTCAGCAGGACCGGCAGACATTCTGAGAGAACCGTGAGCGCC GGCAAGGGCAGCGCCACCTCCAACTGGAAGAAGACGCCCCTCAGCACAGGCGGGACCCTTGCGTTTGTC AGCCCAAGCCTGGCGGTGCACAAGAGCTCCTCGGCCGTGGACCGCCAGCGAGAGTACCTCCTGGACATG ATCCCACCCCGCTCCATCCCCCAGTCAGCCACGTGGAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300946
Insert Size	1905 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300946.1
RefSeq Size	5386 bp
RefSeq ORF	1905 bp
Locus ID	54815
UniProt ID	Q86YP4
Protein Families	Transcription Factors
MW	68.1 kDa
Gene Summary	Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.