ARSL (NM_001282628) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARSE; ASE; CDPX; CDPX1; CDPXR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336999 representing NM_001282628. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGACCCGTGATTAATAGATGTGCTCCAGGCTCCCTTGATTTGATGTTGCCCCAGGCAGCCTCAGAA GGAATTGTATTTCATTCTCTGCAAATCAGTTTGTGTTTCAGGAGCTGGCTGCCAGCGATGCTCGCTGTA CTGCTAAGTTTGGCACCATCAGCTTCCAGCGACATTTCCGCCTCCCGACCGAACATCCTTCTTCTGATG GCGGACGACCTTGGCATTGGGGACATTGGCTGCTATGGCAACAACACCATGAGGACTCCGAATATTGAC CGCCTTGCAGAGGACGGCGTGAAGCTGACCCAACACATCTCTGCCGCATCTTTGTGCACCCCAAGCAGA GCCGCCTTCCTCACGGGCAGATACCCTGTGCGATCAGGGATGGTTTCCAGCATTGGTTACCGTGTTCTT CAGTGGACCGGAGCATCTGGAGGTCTTCCAACAAATGAGACAACTTTTGCAAAAATACTGAAAGAGAAA GGCTATGCCACTGGACTCATTGGAAAATGGCATCTGGGTCTCAACTGTGAGTCAGCCAGTGATCATTGC CACCACCCTCTCCATCATGGCTTTGACCATTTCTACGGAATGCCTTTCTCCTTGATGGGTGATTGCGCC CGCTGGGAACTCTCAGAGAAGCGTGTCAACCTGGAACAAAAACTCAACTTCCTCTTCCAAGTCCTGGCC TTGGTTGCCCTCACACTGGTAGCAGGGAAGCTCACACACCTGATACCCGTCTCGTGGATGCCGGTCATC TGGTCAGCCCTTTCGGCCGTCCTCCTCCTCGCAAGCTCCTATTTTGTGGGTGCTCTGATTGTCCATGCC GATTGCTTTCTGATGAGAAACCACACCATCACGGAGCAGCCCATGTGCTTCCAAAGAACGACACCCCTT ATTCTGCAGGAGGTTGCGTCCTTTCTCAAAAGGAATAAGCATGGGCCTTTCCTCCTCTTTGTTTCCTTT CTACACGTTCACATCCCTCTTATCACTATGGAGAACTTCCTCGGGAAGAGTCTCCACGGGCTGTATGGG GACAACGTAGAGGAGATGGACTGGATGGTAGGACGGATCCTTGACACTTTGGACGTGGAGGGTTTGAGC AACAGCACCCTCATTTATTTTACGTCGGATCACGGCGGTTCCCTAGAGAATCAACTTGGAAACACCCAG TATGGTGGCTGGAATGGAATTTATAAAGGTGGGAAGGGCATGGGAGGATGGGAAGGTGGGATCCGCGTG CCCGGGATCTTCCGCTGGCCCGGGGTGCTCCCGGCCGGCCGAGTGATTGGCGAGCCCACGAGTCTGATG GACGTGTTCCCCACCGTGGTCCGGCTGGCGGGCGGCGAGGTGCCCCAGGACAGAGTGATTGACGGCCAA GACCTTCTGCCCTTGCTCCTGGGGACAGCCCAACACTCAGACCACGAGTTCCTGATGCATTATTGTGAG AGGTTTCTGCACGCAGCCAGGTGGCATCAACGGGACAGAGGAACAATGTGGAAAGTCCACTTTGTGACG CCTGTGTTCCAGCCAGAGGGAGCCGGTGCCTGCTATGGAAGAAAGGTCTGCCCGTGCTTTGGGGAAAAA GTAGTCCACCACGATCCACCTTTGCTCTTTGACCTCTCAAGAGACCCTTCTGAGACCCACATCCTCACA CCAGCCTCAGAGCCCGTGTTCTATCAGGTGATGGAACGAGTCCAGCAGGCGGTGTGGGAACACCAGCGG ACACTCAGCCCAGTTCCTCTGCAGCTGGACAGGCTGGGCAACATCTGGAGACCGTGGCTGCAGCCCTGC TGTGGCCCGTTCCCCCTCTGCTGGTGCCTTAGGGAAGATGACCCACAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282628
Insert Size	1845 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282628.1
RefSeq Size	2506 bp
RefSeq ORF	1845 bp
Locus ID	415
Protein Families	Druggable Genome, Transmembrane
MW	68.2 kDa
Gene Summary	Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the Y chromosome. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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