BRSK2 (NM_001282218) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C11orf7; PEN11B; SAD1; SADA; STK29
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336985 representing NM_001282218. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGCACGAC GTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGTGGTGAGCTCTTCGACTAC CTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTG GACTTCTGCCACAGCCACTCCATATGCCACAGGGATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAG AACAACATCCGCATCGCAGACTTTGGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGC TGTGGGTCCCCCCACTACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGAC GTGTGGAGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAACTTG CGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCGCCCGACTGCCAG AGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCAGAAACAC ATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAGCAGCCCATTCCTCGCAAGGTGCAGATCCGC TCGCTGCCCAGCCTGGAGGACATCGACCCCGACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGA GACCGCAACAAGCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTC CTCCTGGACCGGAAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGAC CCTCCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGCAAATCC ATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCCATTGAGATGGCCCAG CACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTTTCCACCAGCCCACTCAGCAGCCCC CGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCCCTCCCCACCCCCAAGGGGACACCTGTCCACACG CCAAAGGAGAGCCCGGCTGGCACGCCCAACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCC TGGAGGGCGCGGCTCAACTCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTG CAAGTTCCGACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCC TGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGACAAACCTCTG AGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTCATCTCC CAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCAGCCGTGTTCCAGAAGCCGGTCAAGTTC CAGGTTGATATCACCTACACGGAGGGTGGGGAGGCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTC ACCCTGCTCTCAGGCCCCAGCCGTCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGC ACACACGACCCGCCTGCGGCCCAGCACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGT GCTGGGCTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282218
Insert Size	1845 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282218.1
RefSeq Size	4127 bp
RefSeq ORF	1845 bp
Locus ID	9024
UniProt ID	Q8IWQ3
Protein Families	Druggable Genome, Protein Kinase
MW	68.7 kDa
Gene Summary	Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in both UTRs and has multiple differences in the coding region, one of which results in a translational frameshift, compared to variant 1. The resulting protein (isoform 5) has a shorter N-terminus and a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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