AASDH (NM_001286670) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACSF4; LYS2; NRPS998; NRPS1098
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336983 representing NM_001286670. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGTCTAAAGATGCTTCAGTAAAAGAATACATCTTTAAAGAACTGCAGAAATATCTTCCAAGTCAT GCAGTCCCGGATGAGCTTGTATTGATCGACTCTCTACCATTTACATCCCACGGCAAAATTGATGTTTCT GAGTTAAACAAGATATATTTAAACTACATAAACTTGAAGTCTGAGAATAAGCTCAGTGGGAAAGAGGAC CTTTGGGAAAAATTACAGTATTTGTGGAAGTCTACTCTGAATCTCCCAGAAGATCTTTTGAGGGTTCCT GATGAGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTAAAGTCCATCCGGCTCCTCAGTGAGATTGAA AAACTTGTTGGTACATCAGTACCTGGGCTTCTGGAAATTATTCTCAGCAGTTCCATTTTAGAGATTTAT AATCACATCCTTCAAACAGTGGTTCCAGATGAAGATGTGACATTCAGGAAGAGTTGTGCCACAAAAAGG AAACTCAGCGACATTAATCAAGAGGAAGCCAGTGGAACATCTTTACATCAGAAAGCCATCATGACTTTC ACTTGCCACAATGAGATTAATGCTTTTGTTGTACTGAGCAGAGGGAGTCAAATTTTGTCTCTGAATTCC ACTAGGTTTTTAACAAAGTTAGGACATTGCTCTTCAGCCTGTCCTTCTGACTCAGTTTCACAGACCAAC ATTCAAAATTTGAAAGGCTTAAATTCTCCAGTTCTTATTGGGAAGTCAAAAGATCCATCCTGTGTTGCA AAAGTTTCTGAAGAGGGGAAACCTGCGATAGGGACTCAGAAAATGGAGTTACATGTGAGGTGGAGGTCA GACACAGGCAAATGTGTAGATGCTTCACCGCTGGTTGTAATACCCACTTTTGATAAGTCATCTACAACT GTGTACATTGGTTCCCATTCTCATAGAATGAAGGCAGTTGACTTTTACTCTGGGAAGGTAAAATGGGAA CAGATTTTGGGAGATCGAATTGAATCCTCAGCATGTGTATCTAAGTGTGGAAACTTTATTGTGGTGGGC TGTTATAATGGATTAGTTTATGTTCTGAAAAGTAATAGTGGAGAAAAATACTGGATGTTTACTACTGAA GATGCTGTCAAAAGCTCGGCAACCATGGATCCAACCACAGGACTCATTTACATTGGATCTCATGACCAG CACGCATATGCTTTAGATATTTATAGAAAGAAGTGTGTTTGGAAGTCAAAATGTGGAGGAACTGTCTTT TCCTCTCCGTGTTTGAACCTGATTCCACATCATTTGTATTTTGCTACATTGGGAGGACTTTTACTGGCT GTAAATCCTGCTACTGGGAACGTTATTTGGAAACATTCCTGTGGAAAACCACTCTTCTCTTCCCCACAA TGTTGCTCACAGTATATTTGTATTGGCTGTGTAGATGGGAATTTACTCTGCTTTACTCACTTTGGAGAA CAGGTTTGGCAGTTCTCTACCAGTGGACCAATCTTTTCATCCCCGTGTACCTCACCATCAGAGCAAAAA ATATTTTTTGGTTCCCATGATTGCTTTATCTACTGTTGTAACATGAAAGGTCACCTGCAGTGGAAATTT GAAACTACTTCAAGGGTCTATGCAACACCGTTTGCTTTCCATAACTACAATGGCAGCAATGAAATGTTG CTGGCAGCAGCATCTACTGATGGGAAAGTGTGGATCTTGGAATCTCAGAGTGGACAATTGCAAAGTGTT TATGAACTTCCTGGAGAAGTCTTCTCTTCTCCTGTGGTCCTGGAATCAATGCTCATTATTGGGTGTAGA GATAATTATGTTTATTGTCTGGATTTATTGGGTGGCAATCAAAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286670
Insert Size	1842 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286670.1
RefSeq Size	3017 bp
RefSeq ORF	1842 bp
Locus ID	132949
UniProt ID	Q4L235
Protein Pathways	Lysine biosynthesis, Lysine degradation, Metabolic pathways
MW	67.9 kDa
Gene Summary	This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (4) lacks several internal exons and uses a downstream start codon compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1.

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