MCAK (KIF2C) (NM_001297657) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT139; KNSL6; MCAK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336976 representing NM_001297657. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCACTGTCTCAGAGCTTCGCATCACGGCTCAGGAGAATGACATGGAGGTGGAGCTGCCTGCAGCT GCAAACTCCCGCAAGCAGTTTTCAGTTCCTCCTGCCCCCACTAGGCCTTCCTGCCCTGCAGTGGCTGAA ATACCATTGAGGATGGTCAGCGAGGAGATGGAAGAGCAAGTCCATTCCATCCGAGGCAGCTCTTCTGCA AACCCTGTGAACTCAGTTCGGAGGAAATCATGTCTTGTGAAGGAAGTGGAAAAAATGAAGAACAAGCGA GAAGAGAAGAAGGCCCAGAACTCTGAAATGAGAATGAAGAGAGCTCAGGAGTATGACAGTAGTTTTCCA AACTGGGAATTTGCCCGAATGATTAAAGAATTTCGGGCTACTTTGGAATGTCATCCACTTACTATGACT GATCCTATCGAAGAGCACAGAATATGTGTCTGTGTTAGGAAACGCCCACTGAATAAGCAAGAATTGGCC AAGAAAGAAATTGATGTGATTTCCATTCCTAGCAAGTGTCTCCTCTTGGTACATGAACCCAAGTTGAAA GTGGACTTAACAAAGTATCTGGAGAACCAAGCATTCTGCTTTGACTTTGCATTTGATGAAACAGCTTCG AATGAAGTTGTCTACAGGTTCACAGCAAGGCCACTGGTACAGACAATCTTTGAAGGTGGAAAAGCAACT TGTTTTGCATATGGCCAGACAGGAAGTGGCAAGACACATACTATGGGCGGAGACCTCTCTGGGAAAGCC CAGAATGCATCCAAAGGGATCTATGCCATGGCCTCCCGGGACGTCTTCCTCCTGAAGAATCAACCCTGC TACCGGAAGTTGGGCCTGGAAGTCTATGTGACATTCTTCGAGATCTACAATGGGAAGCTGTTTGACCTG CTCAACAAGAAGGCCAAGCTGCGCGTGCTGGAGGACGGCAAGCAACAGGTGCAAGTGGTGGGGCTGCAG GAGCATCTGGTTAACTCTGCTGATGATGTCATCAAGATGATCGACATGGGCAGCGCCTGCAGAACCTCT GGGCAGACATTTGCCAACTCCAATTCCTCCCGCTCCCACGCGTGCTTCCAAATTATTCTTCGAGCTAAA GGGAGAATGCATGGCAAGTTCTCTTTGGTAGATCTGGCAGGGAATGAGCGAGGCGCGGACACTTCCAGT GCTGACCGGCAGACCCGCATGGAGGGCGCAGAAATCAACAAGAGTCTCTTAGCCCTGAAGGAGTGCATC AGGGCCCTGGGACAGAACAAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACACAGGTGCTGAGGGAC TCCTTCATTGGGGAGAACTCTAGGACTTGCATGATTGCCACGATCTCACCAGGCATAAGCTCCTGTGAA TATACTTTAAACACCCTGAGATATGCAGACAGGGTCAAGGAGCTGAGCCCCCACAGTGGGCCCAGTGGA GAGCAGTTGATTCAAATGGAAACAGAAGAGATGGAAGCCTGCTCTAACGGGGCGCTGATTCCAGGCAAT TTATCCAAGGAAGAGGAGGAACTGTCTTCCCAGATGTCCAGCTTTAACGAAGCCATGACTCAGATCAGG GAGCTGGAGGAGAAGGCTATGGAAGAGCTCAAGGAGATCATACAGCAAGGACCAGACTGGCTTGAGCTC TCTGAGATGACCGAGCAGCCAGACTATGACCTGGAGACCTTTGTGAACAAAGCGGAATCTGCTCTGGCC CAGCAAGCCAAGCATTTCTCAGCCCTGCGAGATGTCATCAAGGCCTTGCGCCTGGCCATGCAGCTGGAA GAGCAGGCTAGCAGACAAATAAGCAGCAAGAAACGGCCCCAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001297657
Insert Size	1839 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297657.1
RefSeq Size	2701 bp
RefSeq ORF	1839 bp
Locus ID	11004
UniProt ID	Q99661
Protein Families	Druggable Genome
MW	68.8 kDa
Gene Summary	This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1.

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