MCAK (KIF2C) (NM_001297657) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CT139; KNSL6; MCAK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239082 representing NM_001297657 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACTGTCTCAGAGCTTCGCATCACGGCTCAGGAGAATGACATGGAGGTGGAGCTGCCTGCAGCTG CAAACTCCCGCAAGCAGTTTTCAGTTCCTCCTGCCCCCACTAGGCCTTCCTGCCCTGCAGTGGCTGAAAT ACCATTGAGGATGGTCAGCGAGGAGATGGAAGAGCAAGTCCATTCCATCCGAGGCAGCTCTTCTGCAAAC CCTGTGAACTCAGTTCGGAGGAAATCATGTCTTGTGAAGGAAGTGGAAAAAATGAAGAACAAGCGAGAAG AGAAGAAGGCCCAGAACTCTGAAATGAGAATGAAGAGAGCTCAGGAGTATGACAGTAGTTTTCCAAACTG GGAATTTGCCCGAATGATTAAAGAATTTCGGGCTACTTTGGAATGTCATCCACTTACTATGACTGATCCT ATCGAAGAGCACAGAATATGTGTCTGTGTTAGGAAACGCCCACTGAATAAGCAAGAATTGGCCAAGAAAG AAATTGATGTGATTTCCATTCCTAGCAAGTGTCTCCTCTTGGTACATGAACCCAAGTTGAAAGTGGACTT AACAAAGTATCTGGAGAACCAAGCATTCTGCTTTGACTTTGCATTTGATGAAACAGCTTCGAATGAAGTT GTCTACAGGTTCACAGCAAGGCCACTGGTACAGACAATCTTTGAAGGTGGAAAAGCAACTTGTTTTGCAT ATGGCCAGACAGGAAGTGGCAAGACACATACTATGGGCGGAGACCTCTCTGGGAAAGCCCAGAATGCATC CAAAGGGATCTATGCCATGGCCTCCCGGGACGTCTTCCTCCTGAAGAATCAACCCTGCTACCGGAAGTTG GGCCTGGAAGTCTATGTGACATTCTTCGAGATCTACAATGGGAAGCTGTTTGACCTGCTCAACAAGAAGG CCAAGCTGCGCGTGCTGGAGGACGGCAAGCAACAGGTGCAAGTGGTGGGGCTGCAGGAGCATCTGGTTAA CTCTGCTGATGATGTCATCAAGATGATCGACATGGGCAGCGCCTGCAGAACCTCTGGGCAGACATTTGCC AACTCCAATTCCTCCCGCTCCCACGCGTGCTTCCAAATTATTCTTCGAGCTAAAGGGAGAATGCATGGCA AGTTCTCTTTGGTAGATCTGGCAGGGAATGAGCGAGGCGCGGACACTTCCAGTGCTGACCGGCAGACCCG CATGGAGGGCGCAGAAATCAACAAGAGTCTCTTAGCCCTGAAGGAGTGCATCAGGGCCCTGGGACAGAAC AAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACACAGGTGCTGAGGGACTCCTTCATTGGGGAGAACT CTAGGACTTGCATGATTGCCACGATCTCACCAGGCATAAGCTCCTGTGAATATACTTTAAACACCCTGAG ATATGCAGACAGGGTCAAGGAGCTGAGCCCCCACAGTGGGCCCAGTGGAGAGCAGTTGATTCAAATGGAA ACAGAAGAGATGGAAGCCTGCTCTAACGGGGCGCTGATTCCAGGCAATTTATCCAAGGAAGAGGAGGAAC TGTCTTCCCAGATGTCCAGCTTTAACGAAGCCATGACTCAGATCAGGGAGCTGGAGGAGAAGGCTATGGA AGAGCTCAAGGAGATCATACAGCAAGGACCAGACTGGCTTGAGCTCTCTGAGATGACCGAGCAGCCAGAC TATGACCTGGAGACCTTTGTGAACAAAGCGGAATCTGCTCTGGCCCAGCAAGCCAAGCATTTCTCAGCCC TGCGAGATGTCATCAAGGCCTTGCGCCTGGCCATGCAGCTGGAAGAGCAGGCTAGCAGACAAATAAGCAG CAAGAAACGGCCCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239082 representing NM_001297657 Red=Cloning site Green=Tags(s) MSTVSELRITAQENDMEVELPAAANSRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSAN PVNSVRRKSCLVKEVEKMKNKREEKKAQNSEMRMKRAQEYDSSFPNWEFARMIKEFRATLECHPLTMTDP IEEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEV VYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKL GLEVYVTFFEIYNGKLFDLLNKKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTSGQTFA NSNSSRSHACFQIILRAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQN KAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPSGEQLIQME TEEMEACSNGALIPGNLSKEEEELSSQMSSFNEAMTQIRELEEKAMEELKEIIQQGPDWLELSEMTEQPD YDLETFVNKAESALAQQAKHFSALRDVIKALRLAMQLEEQASRQISSKKRPQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297657
ORF Size	1836 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297657.2
RefSeq Size	2701 bp
RefSeq ORF	1839 bp
Locus ID	11004
UniProt ID	Q99661
Protein Families	Druggable Genome
MW	69.3 kDa
Gene Summary	This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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