EMR3 (ADGRE3) (NM_001289158) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EMR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336942 representing NM_001289158. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGGGACCATTGCTTCTTCCAGGCCTCTGCTTTCTGCTGAGCCTCTTTGGAGCTGTGACTCAGAAA ACCAAAACTTCCTGTGCTAAGTGCCCCCCAAATGCTTCCTGTGTCAATAACACTCACTGCACCTGCAAC CATGGATATACTTCTGGATCTGGGCAGAAACTATTCACATTCCCCTTGGAGACATGTAACGACACCACC TCCTCAAAGACAACCGAGGGCAGGAAAGAGCTGCAAAAGATTGTGGACAAATTTGAGTCACTTCTCACC AATCAGACTTTATGGAGAACAGAAGGGAGACAAGAAATCTCATCCACAGCTACCACTATTCTCCGGGAT GTGGAATCGAAAGTTCTAGAAACTGCCTTGAAAGATCCAGAACAAAAAGTCCTGAAAATCCAAAACGAT AGTGTAGCTATTGAAACTCAAGCGATTACAGACAATTGCTCTGAAGAAAGAAAGACATTCAACTTGAAC GTCCAAATGAACTCAATGGACATCCGTTGCAGTGACATCATCCAGGGAGACACACAAGGTCCCAGTGCC ATTGCCTTTATCTCATATTCTTCTCTTGGAAACATCATAAATGCAACTTTTTTTGAAGAGATGGATAAG AAAGATCAAGTGTATCTGAACTCTCAGGTTGTGAGTGCTGCTATTGGACCCAAAAGGAACGTGTCTCTC TCCAAGTCTGTGACGCTGACTTTCCAGCACGTGAAGATGACCCCCAGTACCAAAAAGGTCTTCTGTGTC TACTGGAAGAGCACAGGGCAGGGCAGCCAGTGGTCCAGGGATGGCTGCTTCCTGATACACGTGAACAAG AGTCACACCATGTGTAATTGCAGTCACCTGTCCAGCTTCGCTGTCCTGATGGCCCTGACCAGCCAGGAG GAGGATCCCGTGCTGACTGTCATCACCTACGTGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCG GCCCTCACTTTTCTCCTGTGTAAAGCCATCCGGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTC TGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGGGATTGATCGAACTGAACCCAAGGTGCTGTGCTCC ATCATCGCCGGTGCTTTGCACTATCTCTACCTGGCCGCCTTCACCTGGATGCTGCTGGAGGGTGTGCAC CTCTTCCTCACTGCACGGAACCTGACAGTGGTCAACTACTCAAGCATCAATAGACTCATGAAGTGGATC ATGTTCCCAGTCGGCTATGGCGTTCCCGCTGTGACTGTGGCCATTTCTGCAGCCTCCTGGCCTCACCTT TATGGAACTGCTGATCGATGCTGGCTCCACCTGGACCAGGGATTCATGTGGAGTTTCCTTGGCCCAGTC TGTGCCATTTTCTCTGCGAATTTAGTATTGTTTATCTTGGTCTTTTGGATTTTGAAAAGAAAACTTTCC TCCCTCAATAGTGAAGTGTCAACCATCCAGAACACAAGGATGCTGGCTTTCAAAGCAACAGCTCAGCTC TTCATCCTGGGCTGCACATGGTGTCTGGGCTTGCTACAGGTGGGTCCAGCTGCCCAGGTCATGGCCTAC CTCTTCACCATCATCAACAGCCTCCAAGGCTTCTTCATCTTCTTGGTCTACTGCCTCCTCAGCCAGCAG GTCCAGAAACAATATCAAAAGTGGTTTAGAGAGATCGTAAAATCAAAATCTGAGTCTGAGACATACACA CTTTCCAGCAAGATGGGTCCTGACTCAAAACCCAGTGAGGGGGATGTTTTTCCAGGACAAGTGAAGAGA AAATATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289158
Insert Size	1803 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289158.1
RefSeq Size	2267 bp
RefSeq ORF	1803 bp
Locus ID	84658
UniProt ID	Q9BY15
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
MW	66.8 kDa
Gene Summary	This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1.

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