ARMC8 (NM_001282342) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GID5; HSPC056; S863-2; VID28
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336941 representing NM_001282342. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAAATGCTGTAATTGGAAACAACAAGCAGAAAGCCAATCTCATTGTTTTAGGAGCTGTTCCAAGA TTGTTGTACTTGCTTCAGCAAGAAACCTCAAGCACAGAGCTGAAAACTGAATGTGCAGTGGTGTTGGGA AGTCTTGCTATGGGTACTGAAAACAATGTCAAGTCTCTACTGGACTGCCATATTATCCCTGCCTTATTG CAAGGACTACTGTCCCCAGACCTGAAGTTTATTGAAGCTTGCCTCCGATGCCTGCGTACCATCTTCACC AGTCCTGTCACTCCAGAGGAGCTACTGTATACAGGGCCAGATCATCAAACAATTTTATTTAACCACGGT GCAGTTCAGAATATTGCTCACCTACTAACCTCACTGTCCTACAAAGTTCGAATGCAAGCACTGAAATGT TTCTCAGTTTTAGCTTTTGAAAACCCCCAGGTATCGATGACCCTGGTAAATGTTTTGGTTGATGGAGAA TTGTTACCACAGATTTTTGTGAAGATGTTACAGAGGGATAAGCCTATTGAGATGCAGCTCACATCAGCA AAATGTTTAACTTACATGTGTAGAGCTGGAGCAATTCGGACAGATGATAACTGTATTGTATTAAAGACA TTACCTTGTTTGGTTCGAATGTGCAGTAAGGAGAGATTACTAGAGGAGAGAGTTGAAGGAGCTGAGACA CTTGCCTATCTGATTGAACCAGATGTTGAGCTACAGAGAATCGCTAGCATAACTGATCACCTCATTGCC ATGCTTGCTGATTATTTCAAGTATCCCAGCTCAGTGAGTGCCATCACTGATATTAAAAGGCTTGATCAT GATTTAAAACATGCTCACGAACTCCGCCAGGCTGCATTCAAGCTCTATGCCTCTCTTGGAGCAAATGAT GAAGACATCCGGAAGAAGATCATTGAGACTGAAAATATGATGGACCGAATTGTGACTGGCTTGTCTGAG TCTAGTGTCAAGGTGCGGTTAGCTGCCGTCAGATGTTTGCACAGTTTATCCAGATCTGTGCAGCAGCTT CGAACCAGTTTCCAGGATCATGCTGTTTGGAAACCTTTAATGAAGGTTTTACAAAATGCACCAGATGAA ATCCTAGTGGTAGCATCTTCCATGCTGTGTAATCTTCTTCTTGAATTTTCTCCAAGCAAAGAGCCAATT TTGGAATCAGGAGCCGTAGAGCTACTTTGTGGATTAACTCAGAGTGAAAATCCTGCTTTACGAGTGAAT GGAATTTGGGCTTTAATGAATATGGCATTTCAGGCTGAACAAAAAATAAAAGCAGATATTTTACGAAGC TTGAGTACTGAACAGCTATTCCGGTTATTATCAGATTCAGATTTGAATGTGCTGATGAAGACATTGGGA CTTCTTAGAAATCTCCTCTCCACTCGTCCTCATATAGATAAAATAATGAGTACTCATGGAAAGCAAATT ATGCAAGCCGTCACTCTTATTCTAGAAGGGGAACATAACATTGAGGTCAAAGAGCAGACACTGTGCATC TTAGCCAACATAGCGGATGGGACAACAGCAAAAGATCTTATTATGACCAATGATGATATCCTACAGAAA ATCAAGTATTACATGGGCCATTCACATGTTAAACTGCAGCTTGCAGCCATGTTTTGTATATCAAACCTC ATATGGAATGAAGAGGAAGGTTCACAAGAACGCCAGGATAAATTACGAGACATGGGCATCGTAGATATT CTACACAAACTGAGTCAGTCACCAGATTCAAACCTTTGTGACAAGGCAAAGATGGCACTGCAGCAGTAC CTGGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282342
Insert Size	1803 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282342.1
RefSeq Size	4669 bp
RefSeq ORF	1803 bp
Locus ID	25852
UniProt ID	Q8IUR7
MW	67.2 kDa
Gene Summary	Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in the 5' and 3' UTRs and has multiple differences in the coding region, including the use of a downstream start codon, compared to variant 1. The encoded isoform (5) is longer and has a shorter N-terminus and distinct C-terminus, compared to isoform 1.

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