ARMC8 (NM_001282342) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GID5; HSPC056; S863-2; VID28
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239047 representing NM_001282342 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAATGCTGTAATTGGAAACAACAAGCAGAAAGCCAATCTCATTGTTTTAGGAGCTGTTCCAAGAT TGTTGTACTTGCTTCAGCAAGAAACCTCAAGCACAGAGCTGAAAACTGAATGTGCAGTGGTGTTGGGAAG TCTTGCTATGGGTACTGAAAACAATGTCAAGTCTCTACTGGACTGCCATATTATCCCTGCCTTATTGCAA GGACTACTGTCCCCAGACCTGAAGTTTATTGAAGCTTGCCTCCGATGCCTGCGTACCATCTTCACCAGTC CTGTCACTCCAGAGGAGCTACTGTATACAGGGCCAGATCATCAAACAATTTTATTTAACCACGGTGCAGT TCAGAATATTGCTCACCTACTAACCTCACTGTCCTACAAAGTTCGAATGCAAGCACTGAAATGTTTCTCA GTTTTAGCTTTTGAAAACCCCCAGGTATCGATGACCCTGGTAAATGTTTTGGTTGATGGAGAATTGTTAC CACAGATTTTTGTGAAGATGTTACAGAGGGATAAGCCTATTGAGATGCAGCTCACATCAGCAAAATGTTT AACTTACATGTGTAGAGCTGGAGCAATTCGGACAGATGATAACTGTATTGTATTAAAGACATTACCTTGT TTGGTTCGAATGTGCAGTAAGGAGAGATTACTAGAGGAGAGAGTTGAAGGAGCTGAGACACTTGCCTATC TGATTGAACCAGATGTTGAGCTACAGAGAATCGCTAGCATAACTGATCACCTCATTGCCATGCTTGCTGA TTATTTCAAGTATCCCAGCTCAGTGAGTGCCATCACTGATATTAAAAGGCTTGATCATGATTTAAAACAT GCTCACGAACTCCGCCAGGCTGCATTCAAGCTCTATGCCTCTCTTGGAGCAAATGATGAAGACATCCGGA AGAAGATCATTGAGACTGAAAATATGATGGACCGAATTGTGACTGGCTTGTCTGAGTCTAGTGTCAAGGT GCGGTTAGCTGCCGTCAGATGTTTGCACAGTTTATCCAGATCTGTGCAGCAGCTTCGAACCAGTTTCCAG GATCATGCTGTTTGGAAACCTTTAATGAAGGTTTTACAAAATGCACCAGATGAAATCCTAGTGGTAGCAT CTTCCATGCTGTGTAATCTTCTTCTTGAATTTTCTCCAAGCAAAGAGCCAATTTTGGAATCAGGAGCCGT AGAGCTACTTTGTGGATTAACTCAGAGTGAAAATCCTGCTTTACGAGTGAATGGAATTTGGGCTTTAATG AATATGGCATTTCAGGCTGAACAAAAAATAAAAGCAGATATTTTACGAAGCTTGAGTACTGAACAGCTAT TCCGGTTATTATCAGATTCAGATTTGAATGTGCTGATGAAGACATTGGGACTTCTTAGAAATCTCCTCTC CACTCGTCCTCATATAGATAAAATAATGAGTACTCATGGAAAGCAAATTATGCAAGCCGTCACTCTTATT CTAGAAGGGGAACATAACATTGAGGTCAAAGAGCAGACACTGTGCATCTTAGCCAACATAGCGGATGGGA CAACAGCAAAAGATCTTATTATGACCAATGATGATATCCTACAGAAAATCAAGTATTACATGGGCCATTC ACATGTTAAACTGCAGCTTGCAGCCATGTTTTGTATATCAAACCTCATATGGAATGAAGAGGAAGGTTCA CAAGAACGCCAGGATAAATTACGAGACATGGGCATCGTAGATATTCTACACAAACTGAGTCAGTCACCAG ATTCAAACCTTTGTGACAAGGCAAAGATGGCACTGCAGCAGTACCTGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239047 representing NM_001282342 Red=Cloning site Green=Tags(s) MKNAVIGNNKQKANLIVLGAVPRLLYLLQQETSSTELKTECAVVLGSLAMGTENNVKSLLDCHIIPALLQ GLLSPDLKFIEACLRCLRTIFTSPVTPEELLYTGPDHQTILFNHGAVQNIAHLLTSLSYKVRMQALKCFS VLAFENPQVSMTLVNVLVDGELLPQIFVKMLQRDKPIEMQLTSAKCLTYMCRAGAIRTDDNCIVLKTLPC LVRMCSKERLLEERVEGAETLAYLIEPDVELQRIASITDHLIAMLADYFKYPSSVSAITDIKRLDHDLKH AHELRQAAFKLYASLGANDEDIRKKIIETENMMDRIVTGLSESSVKVRLAAVRCLHSLSRSVQQLRTSFQ DHAVWKPLMKVLQNAPDEILVVASSMLCNLLLEFSPSKEPILESGAVELLCGLTQSENPALRVNGIWALM NMAFQAEQKIKADILRSLSTEQLFRLLSDSDLNVLMKTLGLLRNLLSTRPHIDKIMSTHGKQIMQAVTLI LEGEHNIEVKEQTLCILANIADGTTAKDLIMTNDDILQKIKYYMGHSHVKLQLAAMFCISNLIWNEEEGS QERQDKLRDMGIVDILHKLSQSPDSNLCDKAKMALQQYLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282342
ORF Size	1800 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282342.2
RefSeq Size	4669 bp
RefSeq ORF	1803 bp
Locus ID	25852
UniProt ID	Q8IUR7
MW	67.6 kDa
Gene Summary	Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1.[UniProtKB/Swiss-Prot Function]

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