CENTG3 (AGAP3) (NM_001281300) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGAP-3; CENTG3; cnt-g3; CRAG; MRIP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336867 representing NM_001281300. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGCTTGTGGGCACGCAGGATGCCATCAGCGCTGCGAATCCCCGGGTTATCGACGACAGCAGAGCC CGCAAGCTCTCCACAGATCTGAAGCGGTGCACCTACTATGAGACGTGCGCGACCTACGGGCTCAATGTG GAGCGTGTCTTCCAGGACGTGGCCCAGAAGGTAGTGGCCTTGCGAAAGAAGCAGCAACTGGCCATCGGG CCCTGCAAGTCACTGCCCAACTCGCCCAGCCACTCGGCCGTGTCCGCCGCCTCCATCCCGGCCGTGCAC ATCAACCAGGCCACGAATGGCGGCGGCAGCGCCTTCAGCGACTACTCGTCCTCAGTCCCCTCCACCCCC AGCATCAGCCAGCGGGAGCTGCGCATCGAGACCATCGCTGCCTCCTCCACCCCCACACCCATCCGAAAG CAGTCCAAGCGGCGCTCCAACATCTTCACGTCTCGGAAGGGTGCTGACCTGGACCGGGAGAAGAAGGCT GCCGAGTGCAAGGTGGACAGCATCGGGAGCGGCCGCGCCATCCCCATCAAGCAGGGGATCCTGCTAAAG CGGAGCGGCAAGTCCCTGAACAAGGAGTGGAAGAAGAAGTATGTGACGCTCTGTGACAACGGGCTGCTC ACCTATCACCCCAGCCTGCATGATTACATGCAGAACATCCACGGCAAGGAGATTGACCTGCTGCGGACA ACGGTGAAAGTGCCAGGGAAGCGCCTGCCCCGAGCCACACCTGCCACAGCCCCGGGCACCAGCCCCCGT GCCAACGGGCTGTCCGTGGAGCGGAGTAACACACAGCTGGGTGGGGGCACAGAGGCAGAGGAGTCGTTT GAATTTGTGGTGGTGTCCCTCACTGGGCAGACGTGGCACTTCGAGGCTTCAACGGCGGAGGAGCGGGAG CTGTGGGTTCAGAGTGTGCAGGCCCAGATCCTTGCCAGCCTGCAAGGCTGCCGCAGTGCCAAGGACAAG ACTCGACTGGGGAACCAGAACGCAGCTCTGGCTGTGCAGGCCGTCCGCACCGTCCGCGGCAACAGCTTT TGTATCGACTGCGATGCACCCAATCCAGACTGGGCCAGCCTGAACCTGGGTGCCCTGATGTGCATTGAG TGCTCAGGCATCCACCGACACCTGGGGGCTCACCTGTCCCGGGTGCGCTCCCTTGACCTCGATGACTGG CCGCCTGAGCTGCTGGCTGTCATGACTGCCATGGGCAATGCCCTCGCCAACAGCGTCTGGGAGGGGGCC TTGGGTGGCTACTCCAAGCCAGGGCCTGATGCCTGCAGAGAGGAGAAGGAACGCTGGATACGGGCCAAG TATGAACAGAAGCTCTTCCTGGCCCCACTGCCAAGCTCAGATGTGCCACTGGGGCAGCAGCTGCTCCGG GCCGTGGTGGAAGATGACCTGCGGCTGTTGGTGATGCTCCTGGCACATGGCTCCAAAGAGGAGGTGAAT GAGACCTATGGGGACGGGGACGGGCGGACGGCTCTACATCTCTCCAGTGCCATGGCCAACGTTGTCTTC ACGCAGCTGCTCATCTGGTACGGGGTGGACGTGAGGAGCCGGGACGCCCGGGGCCTGACTCCACTGGCA TATGCTCGCCGGGCCGGCAGCCAGGAGTGTGCAGACATCTTGATCCAGCATGGCTGCCCTGGGGAGGGC TGTGGCTTAGCGCCTACCCCCAACAGAGAGCCTGCCAATGGCACCAACCCCTCTGCTGAGCTGCACCGT AGTCCTAGCCTCCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281300
Insert Size	1743 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281300.1
RefSeq Size	2783 bp
RefSeq ORF	1743 bp
Locus ID	116988
UniProt ID	Q96P47
MW	62.8 kDa
Gene Summary	This gene encodes an essential component of the N-methyl-D-aspartate (NMDA) receptor signaling complex which mediates long-term potentiation in synapses by linking activation of NMDA receptor to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The encoded protein contains an N-terminal GTPase-like domain, a pleckstrin homology domain, an ArfGAP domain and several C-terminal ankryn repeat domains. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (3) differs in the 5' UTR, lacks two alternate exons in the 3' coding region, and initiates translation from a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a.

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