CEP70 (NM_001288965) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BITE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336855 representing NM_001288965. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACTGAGCAGGAAGAAGCAGAATGGGAAAGCATAAATGTGCTATTGATGATGCATGGCTTAAAACCT TTGTCTCTAGTCAAAAGAACAGATCTCAAAGATCTCATCATTTTTGACAAACAGTCATCACAAAGGATG AGACAGAATTTGAAATTGTTGGTGGAAGAAACATCATGTCAACAGAACATGATACAGGAGCTTATAGAA ACTAATCAACAGCTTAGAAATGAACTTCAGCTAGAGCAAAGCCGAGCAGCCAATCAAGAACAACGAGCT AATGACTTGGAACAAATTATGGAAAGTGTGAAATCCAAAATTGGTGAATTGGAGGATGAATCACTAAGT AGGGCTTGCCACCAACAGAATAAAATAAAAGATCTTCAAAAGGAGCAGAAAACTTTACAGGTGAAGTGC CAGCATTATAAGAAAAAACGAACGGAGCAAGAAGAAACTATTGCTTCTTTGCAAATGGAAGTCTGTAGA TTAAAAAAGGAGGAAGAAGATCGCATTGTCACTCAAAACAGAGTGTTTGCCTATCTGTGCAAAAGAGTT CCTCATACCGTCTTGGATAGACAGTTGCTTTGTCTAATTGATTACTATGAATCTAAAATTAGAAAAATT CATACACAAAGGCAATATAAAGAAGATGAAAGTCAGTCAGAAGAAGAAAACGACTACAGAAATCTGGAT GCCTCACCAACTTATAAAGGCCTTTTAATGTCATTACAGAATCAACTCAAGGAATCAAAATCTAAGATT GATGCACTTTCAAGTGAAAAGCTGAACCTCCAAAAAGATTTGGAAACCAGGCCTACGCAGCATGAATTA AGACTTTATAAACAGCAGGTGAAGAAGCTGGAAAAAGCCCTTAAGAAAAACGTCAAATTACAGGAGCTT ATTAATCATAAGAAGGCTGAGGACACAGAGAAGAAAGATGAGCCCAGCAAATATAATCAGCAACAGGCC CTAATTGACCAGAGATACTTTCAGGTGCTGTGTAGCATCAATTCAATTATCCACAATCCAAGAGCTCCA GTAATAATTTATAAACAGACCAAAGGGGGAGTCCAAAATTTTAATAAAGATCTTGTTCAAGATTGTGGA TTTGAGCATCTTGTTCCTGTAATAGAAATGTGGGCAGATCAACTGACATCCTTAAAGGATTTGTATAAG TCCTTGAAAACACTATCTGCAGAACTGGTACCTTGGCTTAATTTGAAGAAGCAGGATGAAAATGAAGGT ATCAAAGTTGAAGATTTGTTGTTTATAGTAGATACTATGCTGGAAGAAGTTGAAAATAAGGAAAAGGAC AGCAATATGCCACACTTTCAAACTTTGCAAGCTATTGTTTCTCACTTCCAAAAGTTATTTGATGTGCCT TCTTTAAATGGAGTCTATCCCCGAATGAATGAAGTTTATACTAGGCTTGGAGAAATGAACAATGCTGTG AGAAACCTCCAAGAACTCTTAGAATTAGATAGTTCATCCTCATTGTGTGTGCTAGTAAGCACTGTTGGA AAACTCTGTAGGCTGATTAATGAAGATGTGAATGAGCAGGTTATGCAGGTATTAGGACCTGAAGACCTC CAGAGCATTATCTACAAATTGGAAGAACACGAGGAATTTTTCCCAGCATTTCAGGCATTTACTAATGAT CTACTTGAAATCTTAGAAATTGATGACTTGGATGCCATTGTACCTGCAGTAAAGAAATTAAAAGTACTT TCATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288965
Insert Size	1734 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288965.1
RefSeq Size	2697 bp
RefSeq ORF	1734 bp
Locus ID	80321
UniProt ID	Q8NHQ1
MW	67.5 kDa
Gene Summary	Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct N-terminus, compared to isoform 1.

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