CEP70 (NM_001288965) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BITE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238961 representing NM_001288965 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAGCAGGAAGAAGCAGAATGGGAAAGCATAAATGTGCTATTGATGATGCATGGCTTAAAACCTT TGTCTCTAGTCAAAAGAACAGATCTCAAAGATCTCATCATTTTTGACAAACAGTCATCACAAAGGATGAG ACAGAATTTGAAATTGTTGGTGGAAGAAACATCATGTCAACAGAACATGATACAGGAGCTTATAGAAACT AATCAACAGCTTAGAAATGAACTTCAGCTAGAGCAAAGCCGAGCAGCCAATCAAGAACAACGAGCTAATG ACTTGGAACAAATTATGGAAAGTGTGAAATCCAAAATTGGTGAATTGGAGGATGAATCACTAAGTAGGGC TTGCCACCAACAGAATAAAATAAAAGATCTTCAAAAGGAGCAGAAAACTTTACAGGTGAAGTGCCAGCAT TATAAGAAAAAACGAACGGAGCAAGAAGAAACTATTGCTTCTTTGCAAATGGAAGTCTGTAGATTAAAAA AGGAGGAAGAAGATCGCATTGTCACTCAAAACAGAGTGTTTGCCTATCTGTGCAAAAGAGTTCCTCATAC CGTCTTGGATAGACAGTTGCTTTGTCTAATTGATTACTATGAATCTAAAATTAGAAAAATTCATACACAA AGGCAATATAAAGAAGATGAAAGTCAGTCAGAAGAAGAAAACGACTACAGAAATCTGGATGCCTCACCAA CTTATAAAGGCCTTTTAATGTCATTACAGAATCAACTCAAGGAATCAAAATCTAAGATTGATGCACTTTC AAGTGAAAAGCTGAACCTCCAAAAAGATTTGGAAACCAGGCCTACGCAGCATGAATTAAGACTTTATAAA CAGCAGGTGAAGAAGCTGGAAAAAGCCCTTAAGAAAAACGTCAAATTACAGGAGCTTATTAATCATAAGA AGGCTGAGGACACAGAGAAGAAAGATGAGCCCAGCAAATATAATCAGCAACAGGCCCTAATTGACCAGAG ATACTTTCAGGTGCTGTGTAGCATCAATTCAATTATCCACAATCCAAGAGCTCCAGTAATAATTTATAAA CAGACCAAAGGGGGAGTCCAAAATTTTAATAAAGATCTTGTTCAAGATTGTGGATTTGAGCATCTTGTTC CTGTAATAGAAATGTGGGCAGATCAACTGACATCCTTAAAGGATTTGTATAAGTCCTTGAAAACACTATC TGCAGAACTGGTACCTTGGCTTAATTTGAAGAAGCAGGATGAAAATGAAGGTATCAAAGTTGAAGATTTG TTGTTTATAGTAGATACTATGCTGGAAGAAGTTGAAAATAAGGAAAAGGACAGCAATATGCCACACTTTC AAACTTTGCAAGCTATTGTTTCTCACTTCCAAAAGTTATTTGATGTGCCTTCTTTAAATGGAGTCTATCC CCGAATGAATGAAGTTTATACTAGGCTTGGAGAAATGAACAATGCTGTGAGAAACCTCCAAGAACTCTTA GAATTAGATAGTTCATCCTCATTGTGTGTGCTAGTAAGCACTGTTGGAAAACTCTGTAGGCTGATTAATG AAGATGTGAATGAGCAGGTTATGCAGGTATTAGGACCTGAAGACCTCCAGAGCATTATCTACAAATTGGA AGAACACGAGGAATTTTTCCCAGCATTTCAGGCATTTACTAATGATCTACTTGAAATCTTAGAAATTGAT GACTTGGATGCCATTGTACCTGCAGTAAAGAAATTAAAAGTACTTTCATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238961 representing NM_001288965 Red=Cloning site Green=Tags(s) MTEQEEAEWESINVLLMMHGLKPLSLVKRTDLKDLIIFDKQSSQRMRQNLKLLVEETSCQQNMIQELIET NQQLRNELQLEQSRAANQEQRANDLEQIMESVKSKIGELEDESLSRACHQQNKIKDLQKEQKTLQVKCQH YKKKRTEQEETIASLQMEVCRLKKEEEDRIVTQNRVFAYLCKRVPHTVLDRQLLCLIDYYESKIRKIHTQ RQYKEDESQSEEENDYRNLDASPTYKGLLMSLQNQLKESKSKIDALSSEKLNLQKDLETRPTQHELRLYK QQVKKLEKALKKNVKLQELINHKKAEDTEKKDEPSKYNQQQALIDQRYFQVLCSINSIIHNPRAPVIIYK QTKGGVQNFNKDLVQDCGFEHLVPVIEMWADQLTSLKDLYKSLKTLSAELVPWLNLKKQDENEGIKVEDL LFIVDTMLEEVENKEKDSNMPHFQTLQAIVSHFQKLFDVPSLNGVYPRMNEVYTRLGEMNNAVRNLQELL ELDSSSSLCVLVSTVGKLCRLINEDVNEQVMQVLGPEDLQSIIYKLEEHEEFFPAFQAFTNDLLEILEID DLDAIVPAVKKLKVLSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288965
ORF Size	1731 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288965.2
RefSeq Size	2697 bp
RefSeq ORF	1734 bp
Locus ID	80321
UniProt ID	Q8NHQ1
MW	68 kDa
Gene Summary	Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.[UniProtKB/Swiss-Prot Function]

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