MPP2 (NM_001278372) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DLG2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336851 representing NM_001278372. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGGTTGCCGCCACCAACTCTGAAACTGCCATGCAGCAAGTCCTGGACAACTTGGGATCCCTCCCC AGTGCCACGGGGGCTGCAGAGCTGGACCTGATCTTCCTTCGAGGCATTATGGAAAGTCCCATAGTAAGA TCCCTGGCCAAGGTGATAATGGTATTGTGGTTTATGCAGCAGAATGTCTTTGTTCCTATGAAATACATG CTGAAATACTTTGGGGCCCATGAGAGGCTGGAGGAGACGAAGCTGGAGGCCGTGAGAGACAACAACCTG GAGCTGGTGCAGGAGATCCTGCGGGACCTGGCGCAGCTGGCTGAGCAGAGCAGCACAGCCGCCGAGCTG GCCCACATCCTCCAGGAGCCCCACTTCCAGTCCCTCCTGGAGACGCACGACTCTGTGGCCTCAAAGACC TATGAGACACCACCCCCCAGCCCTGGCCTGGACCCTACATTCAGCAACCAGCCTGTACCTCCCGATGCT GTGCGCATGGTGGGCATCCGCAAGACAGCCGGAGAACATCTGGGTGTAACGTTCCGCGTGGAGGGCGGC GAGCTGGTGATCGCGCGCATTCTGCATGGGGGCATGGTGGCTCAACAAGGCCTGCTGCATGTGGGTGAC ATCATCAAGGAGGTGAACGGGCAGCCAGTGGGCAGTGACCCCCGCGCACTGCAGGAGCTCCTGCGCAAT GCCAGTGGCAGTGTCATCCTCAAGATCCTGCCCAGCTACCAGGAGCCCCATCTGCCCCGCCAGGTATTT GTGAAATGTCACTTTGACTATGACCCGGCCCGAGACAGCCTCATCCCCTGCAAGGAAGCAGGCCTGCGC TTCAACGCCGGGGACTTGCTCCAGATCGTAAACCAGGATGATGCCAACTGGTGGCAGGCATGCCATGTC GAAGGGGGCAGTGCTGGGCTCATTCCCAGCCAGCTGCTGGAGGAGAAGCGGAAAGCATTTGTCAAGAGG GACCTGGAGCTGACACCAAACTCAGGGACCCTATGCGGCAGCCTTTCAGGAAAGAAAAAGAAGCGAATG ATGTATTTGACCACCAAGAATGCAGAGTTTGACCGTCATGAGCTGCTCATTTATGAGGAGGTGGCCCGC ATGCCCCCGTTCCGCCGGAAAACCCTGGTACTGATTGGGGCTCAGGGCGTGGGACGGCGCAGCCTGAAG AACAAGCTCATCATGTGGGATCCAGATCGCTATGGCACCACGGTGCCCTACACCTCCCGGCGGCCGAAA GACTCAGAGCGGGAAGGTCAGGGTTACAGCTTTGTGTCCCGTGGGGAGATGGAGGCTGACGTCCGTGCT GGGCGCTACCTGGAGCATGGCGAATACGAGGGCAACCTGTATGGCACACGTATTGACTCCATCCGGGGC GTGGTCGCTGCTGGGAAGGTGTGCGTGCTGGATGTCAACCCCCAGGCGGTGAAGGTGCTACGAACGGCC GAGTTTGTCCCTTACGTGGTGTTCATCGAGGCCCCAGACTTCGAGACCCTGCGGGCCATGAACAGGGCT GCGCTGGAGAGTGGAATATCCACCAAGCAGCTCACGGAGGCGGACCTGAGACGGACAGTGGAGGAGAGC AGCCGCATCCAGCGGGGCTACGGGCACTACTTTGACCTCTGCCTGGTCAATAGCAACCTGGAGAGGACC TTCCGCGAGCTCCAGACAGCCATGGAGAAGCTACGGACAGAGCCCCAGTGGGTGCCTGTCAGCTGGGTG TACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278372
Insert Size	1731 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278372.1
RefSeq Size	4717 bp
RefSeq ORF	1731 bp
Locus ID	4355
UniProt ID	Q14168
Protein Families	Druggable Genome
MW	64.6 kDa
Gene Summary	Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) includes an alternate coding exon and represents use of an alternate promoter, compared to variant 1. It encodes isoform 4 which has a shorter N-terminus compared to isoform 1.

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