TACC2 (NM_001291879) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AZU-1; ECTACC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336849 representing NM_001291879. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCCTGAGGAGGCCAAAGATGAAAAAGACACCCGAGAAACTTGACAACACTCCTGCCTCACCTCCC AGATCCCCTGCTGAACCCAATGACATCCCCATTGCTAAAGGTACTTACACCTTTGATATTGACAAGTGG GATGACCCCAATTTTAACCCTTTTTCTTCCACCTCAAAAATGCAGGAGTCTCCCAAACTGCCCCAACAA TCATACAACTTTGACCCAGACACCTGTGATGAGTCCGTTGACCCCTTTAAGACATCCTCTAAGACCCCC AGCTCACCTTCTAAATCCCCAGCCTCCTTTGAGATCCCAGCCAGTGCTATGGAAGCCAATGGAGTGGAC GGGGATGGGCTAAACAAGCCCGCCAAGAAGAAGAAGACGCCCCTAAAGACTGACACATTTAGGGTGAAA AAGTCGCCAAAACGGTCTCCTCTCTCTGATCCACCTTCCCAGGACCCCACCCCAGCTGCTACACCAGAA ACACCACCAGTGATCTCTGCGGTGGTCCACGCCACAGATGAGGAAAAGCTGGCGGTCACCAACCAGAAG TGGACGTGCATGACAGTGGACCTAGAGGCTGACAAACAGGACTACCCGCAGCCCTCGGACCTGTCCACC TTTGTAAACGAGACCAAATTCAGTTCACCCACTGAGGAGTTGGATTACAGAAACTCCTATGAAATTGAA TATATGGAGAAAATTGGCTCCTCCTTACCTCAGGACGACGATGCCCCGAAGAAGCAGGCCTTGTACCTT ATGTTTGACACTTCTCAGGAGAGCCCTGTCAAGTCATCTCCCGTCCGCATGTCAGAGTCCCCGACGCCG TGTTCAGGGTCAAGTTTTGAAGAGACTGAAGCCCTTGTGAACACTGCTGCGAAAAACCAGCATCCTGTC CCACGAGGACTGGCCCCTAACCAAGAGTCACACTTGCAGGTGCCAGAGAAATCCTCCCAGAAGGAGCTG GAGGCCATGGGCTTGGGCACCCCTTCAGAAGCGATTGAAATTAGAGAGGCTGCTCACCCAACAGACGTC TCCATCTCCAAAACAGCCTTGTACTCCCGCATCGGGACCGCTGAGGTGGAGAAACCTGCAGGCCTTCTG TTCCAGCAGCCCGACCTGGACTCTGCCCTCCAGATCGCCAGAGCAGAGATCATAACCAAGGAGAGAGAG GTCTCAGAATGGAAAGATAAATATGAAGAAAGCAGGCGGGAAGTGATGGAAATGAGGAAAATAGTGGCC GAGTATGAGAAGACCATCGCTCAGATGATAGAGGACGAACAGAGAGAGAAGTCAGTCTCCCACCAGACG GTGCAGCAGCTGGTTCTGGAGAAGGAGCAAGCCCTGGCCGACCTGAACTCCGTGGAGAAGTCTCTGGCC GACCTCTTCAGAAGATATGAGAAGATGAAGGAGGTCCTAGAAGGCTTCCGCAAGAATGAAGAGGTGTTG AAGAGATGTGCGCAGGAGTACCTGTCCCGGGTGAAGAAGGAGGAGCAGAGGTACCAGGCCCTGAAGGTG CACGCGGAGGAGAAACTGGACAGGGCCAATGCTGAGATTGCTCAGGTTCGAGGCAAGGCCCAGCAGGAG CAAGCCGCCCACCAGGCCAGCCTGCGGAAGGAGCAGCTGCGAGTGGACGCCCTGGAAAGGACGCTGGAG CAGAAGAATAAAGAAATAGAAGAACTCACCAAGATTTGTGACGAACTGATTGCCAAAATGGGGAAAAGC TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291879
Insert Size	1728 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291879.1
RefSeq Size	3709 bp
RefSeq ORF	1728 bp
Locus ID	10579
MW	64.7 kDa
Gene Summary	Transforming acidic coiled-coil proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. This gene encodes a protein that concentrates at centrosomes throughout the cell cycle. This gene lies within a chromosomal region associated with tumorigenesis. Expression of this gene is induced by erythropoietin and is thought to affect the progression of breast tumors. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (8) differs in the 5' UTR and coding region and lacks two internal coding exons compared to variant 1. These differences result in the use of a downstream AUG compared to variant 1. The encoded isoform (h) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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