Coiled coil domain containing protein 111 (PRIMPOL) (NM_001300768) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCDC111; MYP22; Primpol1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336788 representing NM_001300768. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATAGAAAATGGGAAGCAAAACTGAAGCAAATTGAAGAACGAGCATCTCATTATGAGAGGAAACCG TTGTCCTCAGTGTATAGACCAAGATTGTCCAAGCCAGAAGAACCACCCTCCATCTGGAGACTATTTCAT CGACAAGCTCAAGCTTTTAATTTTGTTAAAAGCTGTAAAGAAGACGTTCATGTATTTGCTTTGGAATGC AAAGTAGGAGATGGACAACGTATTTACCTTGTGACAACCTATGCTGAATTTTGGTTTTACTATAAATCC AGAAAAAATCTCTTACACTGCTATGAAGTTATTCCTGAAAATGCTGTGTGCAAGCTTTATTTTGATTTG GAATTTAACAAACCTGCCAACCCAGGAGCTGATGGGAAAAAGATGGTTGCATTACTCATTGAGTATGTG TGTAAAGCACTTCAAGAGTTATACGGTGTTAATTGCTCAGCTGAAGATGTTTTGAACTTGGATTCTAGC ACTGATGAAAAATTCAGCCGGCATTTAATATTTCAGCTCCATGATGTGGCATTTAAAGATAATATTCAT GTTGGTAATTTTTTGAGAAAAATTTTGCAGCCTGCTCTTGACTTGCTTGGCAGTGAAGATGATGATAGC GCTCCAGAGACAACAGGCCATGGATTTCCCCATTTTTCAGAAGCACCTGCAAGACAAGGATTTTCTTTC AATAAAATGTTCACAGAAAAGGCTACAGAGGAAAGCTGGACATCGAATTCAAAGAAACTGGAGAGGCTG GGGTCAGCTGAGCAAAGCAGTCCTGACCTTTCATTTCTAGTTGTGAAGAATAACATGGGAGAGAAGCAT CTTTTTGTAGATCTCGGAGTTTATACAAGAAATAGAAACTTTCGGCTATATAAATCATCAAAAATTGGA AAGCGTGTGGCTTTGGAGGTTACTGAAGATAACAAATTTTTTCCTATACAGTCAAAAGATGTTTCTGAC GAATATCAATATTTTCTCTCTTCTTTGGTCAGCAATGTCAGGTTCTCAGATACTTTACGAATTCTTACA TGTGAGCCATCTCAGAATAAACAAAAAGGAGTTGGATATTTTAACAGTATCGGCACTTCAGAAACCATT GAAGGTTTTCAGTGTTCTCCCTATCCTGAAGTTGATCATTTTGTTCTTTCTTTGGTGAATAAAGATGGC ATTAAAGGAGGAATTCGGCGTTGGAACTACTTTTTCCCAGAAGAATTACTGGTTTATGATATTTGTAAA TATCGGTGGTGTGAAAACATTGGAAGAGCCCATAAGAGTAATAATATAATGATTCTGGTTGATCTGAAA AATGAAGTTTGGTATCAAAAATGTCATGACCCTGTATGTAAAGCAGAAAACTTCAAATCTGACTGTTTC CCATTACCTGCTGAAGTATGTCTCCTGTTTCTTTTCAAAGAGGAAGAAGAGTTTACAACAGATGAAGCA GATGAAACTAGGAGCAATGAAACCCAGAATCCTCATAAACCATCACCTAGCAGGCTGTCAACAGGTGCA TCTGCTGATGCTGTCTGGGATAATGGCATTGATGATGCTTATTTTTTAGAAGCTACTGAAGATGCTGAA TTAGCTGAAGCTGCAGAGAACAGTCTTCTCAGTTATAACAGTGAAGTGGATGAAATTCCTGATGAACTA ATTATAGAAGTATTACAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300768
Insert Size	1680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300768.1
RefSeq Size	2308 bp
RefSeq ORF	1680 bp
Locus ID	201973
UniProt ID	Q96LW4
MW	64.3 kDa
Gene Summary	This gene encodes a DNA primase-polymerase that belongs to a superfamily of archaeao-eukaryotic primases. Members of this family have primase activity, catalyzing the synthesis of short RNA primers that serve as starting points for DNA synthesis, as well as DNA polymerase activity. The encoded protein facilitates DNA damage tolerance by mediating uninterrupted fork progression after UV irradiation and reinitiating DNA synthesis. An allelic variant in this gene is associated with myopia 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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