CNOT6 (NM_001303241) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCR4; Ccr4a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336783 representing NM_001303241. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCAAAGAAAAATACGAGCCCCCTGACCCTCGGAGGATGTATACAATTATGTCTTCTGAGGAAGCA GCAAATGGAAAGAAATCCCACTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCGCATCT TTGTGGTCACTAACTCACCTGACAGCTTTGCATTTGAGTGACAATTCCCTGTCCCGAATTCCTTCAGAC ATTGCCAAGCTTCACAATCTGGTGTATTTGGACCTGTCATCTAATAAAATTCGTAGCTTACCCGCAGAA CTCGGAAACATGGTATCACTCAGGGAGCTCCATTTAAATAACAACCTGTTACGAGTTCTACCTTTTGAG CTGGGAAAACTGTTTCAGTTGCAGACTTTAGGCCTGAAAGGAAATCCCCTTACCCAGGATATATTGAAC CTTTATCAGGAACCAGATGGAACAAGACGGCTGCTGAACTATTTGCTTGATAATTTGTCAGGTACTGCA AAAAGAATTACAACAGAACAACCACCTCCAAGGTCTTGGATTATGTTACAAGAACCAGATAGGACAAGG CCAACTGCCTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATAC GGCTACTGTCCATCATGGGCGCTAAACTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATCTTGAGC TGCAATGCTGATATCGTAAGTCTTCAGGAGGTTGAAACGGAACAGTATTACAGTTTTTTTCTGGTAGAG CTGAAAGAACGTGGCTATAATGGATTCTTCAGTCCTAAGTCTAGAGCTAGGACAATGTCAGAACAAGAA AGGAAACATGTTGATGGCTGTGCAATATTCTTCAAGACAGAAAAATTTACTTTGGTTCAGAAACACACT GTTGAATTTAATCAGCTAGCCATGGCAAATTCTGAGGGGTCTGAAGCTATGCTGAACAGAGTCATGACA AAAGATAACATTGGGGTTGCAGTACTGCTAGAACTTCGGAAGGAATCGATTGAAATGCCGTCCGGAAAG CCACATCTTGGAACAGAAAAACAACTTATTCTTGTGGCTAACGCCCACATGCATTGGGACCCTGAATAC TCTGATGTGAAGTTGGTACAAACTATGATGTTCCTCTCAGAAGTGAAGAACATTATTGATAAAGCCTCT CGCAACCTCAAATCCAGTGTTTTGGGAGAATTTGGAACTATTCCACTTGTGTTATGTGCAGATCTTAAT TCTTTGCCAGACTCTGGTGTTGTAGAATATTTGAGCACAGGTGGAGTAGAAACAAATCACAAAGACTTT AAGGAGTTGAGGTATAATGAAAGTCTCACAAACTTCAGCTGTCATGGGAAGAATGGAACCACCAATGGA AGGATCACTCATGGTTTCAAGTTACAGAGTGCCTATGAGAGTGGCCTGATGCCTTACACGAATTACACA TTTGATTTCAAGGGTATAATAGACTACATTTTCTATTCTAAACCTCAGCTGAACACCTTAGGCATCCTG GGCCCTCTGGACCACCACTGGCTGGTTGAGAATAACATCAGTGGCTGCCCGCACCCCCTCATCCCCTCT GACCACTTCTCACTTTTTGCACAACTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTCAACGGCATCCAC CTTCCTGGCAGGAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303241
Insert Size	1674 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303241.1
RefSeq Size	6347 bp
RefSeq ORF	1674 bp
Locus ID	57472
Protein Families	Transcription Factors
Protein Pathways	RNA degradation
MW	63.3 kDa
Gene Summary	This gene encodes the catalytic component of the CCR4-NOT core transcriptional regulation complex. The encoded protein has a 3'-5' RNase activity and prefers polyadenylated substrates. The CCR4-NOT complex plays a role in many cellular processes, including miRNA-mediated repression, mRNA degradation, and transcriptional regulation. [provided by RefSeq, Dec 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.