CDC25C (NM_001287583) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDC25; PPP1R60
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336756 representing NM_001287583. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGGCGCAGCTCCGTGTCGTGTCTCTCTCAAAGTGTCTCTCGGACAGCGCTGTTGGGAACGAGCC TGGCGCTTTGATTTAGGGATGGGGGTGGTTTCCTCACTAACGAGTCACCCCCTCCAAAATATCGCAGAA AGTGGACGGCCCCAGTTGGAGGCACATCTTGAAACCCGCGGGGGAGGCCATGTCGGAAGAAGGTTTTGT TTTTCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGC TCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACT TCCTTTACCGTCTGTCCAGATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTA AGCATTTTGTCTGGAGGAACCCCAAAACGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACT GCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAA GTGCATTTAGCTGGGATGAATCATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGC ACTCCGAATGGTTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAA AATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAA TTGGATAAAAATCCAAACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCC CTGAAAGATCAAGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAACTTG AACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAG TATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATT ACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGGCACCTGATTGGTGATTTTTCCAAGGTATGT GCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTA CTGTCGGGGAAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTAT CTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAG AAGCCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAG AGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAGTATCCTGCATTGTAC TACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTCTTTCCAGAATATATGGAACTGTGTGAA CCACAGAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGC AAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287583
Insert Size	1656 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287583.1
RefSeq Size	2304 bp
RefSeq ORF	1656 bp
Locus ID	995
Protein Families	Druggable Genome, Phosphatase, Stem cell - Pluripotency
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
MW	61.9 kDa
Gene Summary	This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (4) differs in the 5' UTR, uses an alternate splice site in the 5' region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (c) has a longer N-terminus than isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.