OSBP2 (NM_001282740) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HLM; ORP-4; ORP4; OSBPL1; OSBPL4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336751 representing NM_001282740. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCAACGCCTGCAGGGACTTCTTGGAACTAGCAGAGATACACAGTCGGAAATGGCAGCGGGCACTG CAGTATGAGCAGGAGCAGCGCGTGCACTTGGAGGAAACCATTGAGCAGCTGGCGAAGCAGCACAACAGC CTCGAGCGGGCCTTCCACAGTGCCCCTGGCCGGCCGGCCAACCCCTCCAAGAGCTTCATTGAGGGAAGC CTCTTGACTCCCAAAGGAGAGGACAGTGAGGAAGATGAAGATACCGAGTACTTTGATGCCATGGAAGAC TCCACATCCTTCATCACCGTGATCACCGAGGCCAAGGAAGACAGAAAAGCTGAAGGTAGCACCGGGACA AGTTCCGTGGACTGGAGCTCAGCAGACAATGTACTAGATGGTGCCTCGCTCGTGCCCAAGGGTTCATCC AAAGTCAAGAGGCGAGTCCGCATTCCCAACAAGCCCAACTACAGCCTTAACCTCTGGAGCATCATGAAG AACTGCATCGGCCGGGAGCTCTCCAGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTC CAGCGGCTGACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCAGTGCACTGCACCAGCTCAGTG GAGCAGATGTGCCTGGTGGCCGCCTTCTCTGTGTCCTCCTACTCCACCACAGTGCACCGCATCGCCAAG CCCTTCAACCCCATGCTGGGGGAGACCTTCGAGCTGGACCGCCTCGACGACATGGGCCTGCGCTCCCTC TGTGAGCAGGTGAGCCACCACCCCCCCTCAGCTGCGCACTACGTGTTCTCCAAGCATGGCTGGAGCCTC TGGCAGGAGATCACCATCTCCAGCAAGTTCCGGGGAAAATACATCTCCATCATGCCGCTAGGTGCCATC CACTTAGAATTCCAGGCCAGTGGGAATCACTACGTGTGGAGGAAGAGCACCTCAACTGTTCACAACATC ATCGTGGGCAAGCTCTGGATCGACCAGTCAGGGGACATCGAGATTGTGAACCATAAGACCAATGACCGG TGCCAGCTGAAGTTCCTGCCCTACAGCTACTTCTCCAAAGAGGCAGCCCGGAAGGTGACAGGAGTGGTG AGTGACAGCCAGGGCAAGGCCCATTACGTGCTGTCCGGCTCGTGGGATGAACAAATGGAGTGCTCCAAG GTCATGCATAGCAGTCCCAGCAGCCCCAGCTCTGACGGGAAGCAGAAGACAGTGTACCAGACCCTGTCA GCCAAGCTGCTGTGGAAGAAGTACCCGCTGCCGGAGAACGCGGAGAACATGTACTACTTCTCAGAGCTG GCCCTGACCCTCAACGAGCACGAGGAGGGCGTAGCGCCAACCGACAGCCGCCTGCGGCCCGACCAGCGG CTGATGGAGAAGGGCCGTTGGGACGAGGCCAATACCGAGAAGCAGCGGCTGGAGGAGAAGCAGCGCCTG TCGCGGCGCCGGCGGCTGGAGGCCTGCGGGCCGGGCAGCAGCTGCAGCTCGGAGGAAGAGAAGGAGGCG GATGCCTACACGCCACTGTGGTTTGAGAAGAGGCTGGATCCGCTGACCGGGGAGATGGCCTGTGTGTAC AAGGGCGGCTACTGGGAGGCCAAGGAGAAGCAAGACTGGCATATGTGCCCCAACATCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282740
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282740.1
RefSeq Size	3529 bp
RefSeq ORF	1650 bp
Locus ID	23762
UniProt ID	Q969R2
MW	62.7 kDa
Gene Summary	The protein encoded by this gene contains a pleckstrin homology (PH) domain and an oxysterol-binding region. It binds oxysterols such as 7-ketocholesterol and may inhibit their cytotoxicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an alternate in-frame splice site, compared to variant 1. It represents use of an internal promoter and initiates translation at a downstream in-frame start codon. The encoded isoform (e) is shorter than isoform a.

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