OSBP2 (NM_001282740) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HLM; ORP-4; ORP4; OSBPL1; OSBPL4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238857 representing NM_001282740 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAACGCCTGCAGGGACTTCTTGGAACTAGCAGAGATACACAGTCGGAAATGGCAGCGGGCACTGC AGTATGAGCAGGAGCAGCGCGTGCACTTGGAGGAAACCATTGAGCAGCTGGCGAAGCAGCACAACAGCCT CGAGCGGGCCTTCCACAGTGCCCCTGGCCGGCCGGCCAACCCCTCCAAGAGCTTCATTGAGGGAAGCCTC TTGACTCCCAAAGGAGAGGACAGTGAGGAAGATGAAGATACCGAGTACTTTGATGCCATGGAAGACTCCA CATCCTTCATCACCGTGATCACCGAGGCCAAGGAAGACAGAAAAGCTGAAGGTAGCACCGGGACAAGTTC CGTGGACTGGAGCTCAGCAGACAATGTACTAGATGGTGCCTCGCTCGTGCCCAAGGGTTCATCCAAAGTC AAGAGGCGAGTCCGCATTCCCAACAAGCCCAACTACAGCCTTAACCTCTGGAGCATCATGAAGAACTGCA TCGGCCGGGAGCTCTCCAGGATCCCCATGCCGGTGAACTTCAATGAGCCCCTGTCCATGCTCCAGCGGCT GACAGAGGACCTGGAGTACCACCACCTGCTGGACAAGGCAGTGCACTGCACCAGCTCAGTGGAGCAGATG TGCCTGGTGGCCGCCTTCTCTGTGTCCTCCTACTCCACCACAGTGCACCGCATCGCCAAGCCCTTCAACC CCATGCTGGGGGAGACCTTCGAGCTGGACCGCCTCGACGACATGGGCCTGCGCTCCCTCTGTGAGCAGGT GAGCCACCACCCCCCCTCAGCTGCGCACTACGTGTTCTCCAAGCATGGCTGGAGCCTCTGGCAGGAGATC ACCATCTCCAGCAAGTTCCGGGGAAAATACATCTCCATCATGCCGCTAGGTGCCATCCACTTAGAATTCC AGGCCAGTGGGAATCACTACGTGTGGAGGAAGAGCACCTCAACTGTTCACAACATCATCGTGGGCAAGCT CTGGATCGACCAGTCAGGGGACATCGAGATTGTGAACCATAAGACCAATGACCGGTGCCAGCTGAAGTTC CTGCCCTACAGCTACTTCTCCAAAGAGGCAGCCCGGAAGGTGACAGGAGTGGTGAGTGACAGCCAGGGCA AGGCCCATTACGTGCTGTCCGGCTCGTGGGATGAACAAATGGAGTGCTCCAAGGTCATGCATAGCAGTCC CAGCAGCCCCAGCTCTGACGGGAAGCAGAAGACAGTGTACCAGACCCTGTCAGCCAAGCTGCTGTGGAAG AAGTACCCGCTGCCGGAGAACGCGGAGAACATGTACTACTTCTCAGAGCTGGCCCTGACCCTCAACGAGC ACGAGGAGGGCGTAGCGCCAACCGACAGCCGCCTGCGGCCCGACCAGCGGCTGATGGAGAAGGGCCGTTG GGACGAGGCCAATACCGAGAAGCAGCGGCTGGAGGAGAAGCAGCGCCTGTCGCGGCGCCGGCGGCTGGAG GCCTGCGGGCCGGGCAGCAGCTGCAGCTCGGAGGAAGAGAAGGAGGCGGATGCCTACACGCCACTGTGGT TTGAGAAGAGGCTGGATCCGCTGACCGGGGAGATGGCCTGTGTGTACAAGGGCGGCTACTGGGAGGCCAA GGAGAAGCAAGACTGGCATATGTGCCCCAACATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238857 representing NM_001282740 Red=Cloning site Green=Tags(s) MINACRDFLELAEIHSRKWQRALQYEQEQRVHLEETIEQLAKQHNSLERAFHSAPGRPANPSKSFIEGSL LTPKGEDSEEDEDTEYFDAMEDSTSFITVITEAKEDRKAEGSTGTSSVDWSSADNVLDGASLVPKGSSKV KRRVRIPNKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDKAVHCTSSVEQM CLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRLDDMGLRSLCEQVSHHPPSAAHYVFSKHGWSLWQEI TISSKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTSTVHNIIVGKLWIDQSGDIEIVNHKTNDRCQLKF LPYSYFSKEAARKVTGVVSDSQGKAHYVLSGSWDEQMECSKVMHSSPSSPSSDGKQKTVYQTLSAKLLWK KYPLPENAENMYYFSELALTLNEHEEGVAPTDSRLRPDQRLMEKGRWDEANTEKQRLEEKQRLSRRRRLE ACGPGSSCSSEEEKEADAYTPLWFEKRLDPLTGEMACVYKGGYWEAKEKQDWHMCPNIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282740
ORF Size	1647 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282740.2
RefSeq Size	3529 bp
RefSeq ORF	1650 bp
Locus ID	23762
UniProt ID	Q969R2
MW	63.2 kDa
Gene Summary	The protein encoded by this gene contains a pleckstrin homology (PH) domain and an oxysterol-binding region. It binds oxysterols such as 7-ketocholesterol and may inhibit their cytotoxicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2013]

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