RPC62 (POLR3C) (NM_001303456) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C82; RPC3; RPC62
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336737 representing NM_001303456. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGATGGACGAAATAGCTCTCAGACTCCCCAGTACAATGACTCAAGCAGAAATTAAGCTCTGTTCT TTGTTGCTGCAAGAGCATTTTGGAGAGATTGTAGAAAAAATTGGAGTCCATCTGATAAGAACCGGCAGC CAGCCACTAAGAGTAATTGCCCATGACACAGGAACATCACTGGATCAGGTGAAGAAAGCCCTGTGTGTC CTCGTCCAACATAACCTGGTGAGTTATCAAGTGCACAAACGTGGTGTGGTGGAGTATGAAGCCCAGTGC AGCCGGGTATTGCGAATGCTTAGATATCCCCGGTACATCTATACTACCAAAACTCTGTACAGTGACACT GGAGAGCTGATTGTTGAGGAGCTTCTGTTGAACGGCAAACTGACAATGTCAGCTGTTGTGAAGAAAGTG GCAGACCGGCTCACAGAGACCATGGAGGATGGCAAGACCATGGACTATGCTGAAGTATCAAACACATTT GTGCGACTGGCAGACACACACTTTGTACAACGCTGCCCTTCGGTACCTACCACTGAGAATTCAGACCCT GGGCCACCACCACCTGCCCCCACACTTGTCATTAATGAAAAGGACATGTACCTGGTTCCTAAACTCAGC TTGATAGGGAAAGGTAAAAGGAGGAGATCATCTGATGAAGATGCTGCTGGGGAGCCCAAGGCCAAGAGA CCAAAATATACTACAGATAACAAGGAGCCCATTCCAGATGATGGGATTTATTGGCAGGCCAACCTTGAC AGATTCCACCAACACTTCCGTGACCAAGCCATTGTGAGCGCAGTTGCTAACAGGATGGACCAGACAAGC AGCGAGATTGTGCGAACCATGCTCCGAATGAGTGAGATTACCACTTCCTCTAGTGCTCCCTTCACCCAG CCATTGTCTTCCAATGAGATCTTCAGATCCCTACCTGTTGGCTATAACATCTCTAAGCAAGTTCTTGAT CAGTATCTCACTCTGCTGGCAGATGATCCACTAGAGTTTGTTGGAAAGTCTGGCGACAGTGGTGGAGGA ATGTATGTCATCAACCTCCATAAGGCATTAGCATCCCTAGCCACAGCCACTCTGGAGTCCGTCGTACAG GAGAGATTTGGGTCTCGCTGTGCTAGAATATTCCGTCTAGTTTTGCAGAAGAAACACATAGAGCAGAAG CAAGTGGAAGACTTTGCAATGATTCCTGCAAAGGAGGCAAAGGATATGCTATATAAGATGCTCTCAGAA AATTTCATGTCACTCCAGGAAATTCCCAAAACACCAGACCATGCCCCATCCAGGACCTTCTATTTATAT ACTGTGAACATCCTGTCAGCTGCCCGAATGTTGTTGCACAGGTGCTACAAGAGCATAGCCAACTTGATA GAAAGGAGGCAATTTGAAACCAAAGAGAATAAGCGTCTACTAGAAAAATCTCAGAGGGTAGAAGCCATC ATTGCATCTATGCAGGCTACTGGTGCAGAGGAAGCACAGTTACAAGAAATAGAGGAGATGATCACAGCT CCTGAACGTCAGCAGCTAGAGACCCTGAAACGTAATGTCAACAAGTTGGATGCCAGTGAGATCCAGGTG GACGAAACCATCTTCCTGCTGGAGTCTTACATTGAGTGCACCATGAAGAGACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303456
Insert Size	1644 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303456.1
RefSeq Size	3722 bp
RefSeq ORF	1644 bp
Locus ID	10623
UniProt ID	Q9BUI4
Protein Families	Transcription Factors
Protein Pathways	Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
MW	62.2 kDa
Gene Summary	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate 5' terminal exon and uses an upstream start codon compared to variant 1. It encodes isoform 2 which has a longer and distinct N-terminus compared to isoform 1.

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