GGT5 (NM_001302464) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GGL; GGT-REL; GGT 5; GGTLA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336652 representing NM_001302464. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCGGGGCTACGGGGCCACGGTCAGCCTAGTCCTGCTGGGTCTGGGGCTGGCGCTGGCTGTCATT GTGCTGGCTGTGGTCCTCTCTCGACACCAGGCCCCATGTGGCCCCCAGGCCTTTGCCCACGCTGCTGTT GCCGCCGACTCCAAGGTCTGCTCGGATATTGGACGAGCCATCCTCCAGCAGCAGGGCTCACCCGTGGAT GCCACCATCGCGGCTCTGGTCTGCACCAGCGTCGTCAACCCTCAGAGCATGGGCCTGGGCGGAGGGGTC ATCTTCACCATCTACAATGTGACAACAGGGGCCCAGTGGATCGGGGTGCCCGGGGAGCTCCGTGGCTAT GCCGAGGCCCACCGCCGCCATGGCCGCCTGCCCTGGGCGCAGCTGTTCCAGCCCACCATCGCGCTGCTC CGAGGGGGGCATGTGGTGGCCCCTGTCCTCAGCCGTTTCCTGCACAACAGCATCCTGCGGCCTTCCTTG CAGGCGTCAACCCTGCGCCAGCTCTTCTTCAACGGGACAGAACCCCTGAGGCCTCAGGACCCACTCCCA TGGCCTGCACTGGCCACCACCCTGGAGACCGTGGCCACAGAGGGCGTGGAGGTCTTCTACACGGGGAGG CTGGGCCAGATGCTGGTGGAGGACATTGCCAAGGAAGGGAGCCAGCTGACGCTGCAGGACCTGGCCAAG TTCCAGCCCGAGGTGGTGGATGCCCTGGAGGTGCCCCTGGGGGACTATACCCTGTACTCACCACCGCCG CCTGCAGGGGGTGCCATTCTCAGCTTTATCCTCAACGTGCTAAGAGGGTTCAACTTCTCAACAGAGTCT ATGGCCAGGCCTGAAGGGAGGGTGAACGTGTACCACCACCTTGTAGAGACGCTCAAGTTTGCCAAGGGG CAGAGGTGGAGGCTGGGGGACCCTCGAAGCCACCCGAAGCTCCAGAATGCCTCCCGGGACCTGCTGGGG GAGACCCTGGCCCAGCTCATCCGCCAACAGATCGATGGCCGGGGGGACCACCAGCTCAGCCACTACAGC TTGGCCGAGGCCTGGGGCCACGGGACAGGCACGTCCCATGTGTCTGTGCTGGGGGAGGATGGCAGCGCC GTGGCTGCCACCAGCACCATCAACACACCCTTTGGAGCGATGGTGTATTCACCACGGACAGGCATCATC CTCAACAACGAGCTCCTGGACTTATGCGAGCGATGCCCCCGGGGTTCCGGCACCACCCCCTCACCTGTG AGTGGAGACAGGGTGGGTGGAGCTCCCGGAAGGTGCTGGCCCCCAGTTCCAGGCGAGCGTTCCCCATCC TCCATGGTGCCCTCCATCTTGATCAACAAAGCCCAGGGGTCGAAGCTAGTGATTGGCGGGGCTGGCGGG GAGCTCATCATCTCTGCTGTGGCCCAGGCCATCATGAGCAAGCTGTGGCTTGGCTTTGACCTGAGAGCG GCCATTGCAGCCCCCATCCTGCATGTCAACAGCAAGGGCTGTGTGGAGTACGAGCCCAACTTCAGCCAG AGACGGTGTCTTGCTTTGTTGCCCTGGCTGTTCTCAAACTCCTGGCCTCAAGTGATCCTGCCACCTTGG CTCCCAAGTGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001302464
Insert Size	1602 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302464.1
RefSeq Size	2507 bp
RefSeq ORF	1602 bp
Locus ID	2687
UniProt ID	P36269
Protein Families	Protease, Transmembrane
Protein Pathways	Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
MW	56.9 kDa
Gene Summary	This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (4) has multiple differences in the coding region, one of which results in a translational frameshift, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1.

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