Glucose 6 phosphate isomerase (GPI) (NM_001289790) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AMF; GNPI; NLK; PGI; PHI; SA-36; SA36
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336632 representing NM_001289790. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCTCTCACCCGGGACCCCCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCACCGCTCCGAG CTGAACCTGCGCCGCCTCTTCGATGCCAACAAGGACCGCTTCAACCACTTCAGCTTGACCCTCAACACC AACCATGGGCATATCCTGGTGGATTACTCCAAGAACCTGGTGACGGAGGACGTGATGCGGATGCTGGTG GACTTGGCCAAGTCCAGGGGCGTGGAGGCCGCCCGGGAGCGGATGTTCAATGGTGAGAAGATCAACTAC ACCGAGGGTCGAGCCGTGCTGCACGTGGCTCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGC AAGGATGTGATGCCAGAGGTCAACAAGGTTCTGGACAAGATGAAGTCTTTCTGCCAGGGACCCCTCATG GTGACTGAAGCCCTTAAGCCATACTCTTCAGGAGGTCCCCGCGTCTGGTATGTCTCCAACATTGATGGA ACTCACATTGCCAAAACCCTGGCCCAGCTGAACCCCGAGTCCTCCCTGTTCATCATTGCCTCCAAGACC TTTACTACCCAGGAGACCATCACGAATGCAGAGACGGCGAAGGAGTGGTTTCTCCAGGCGGCCAAGGAT CCTTCTGCAGTGGCGAAGCACTTTGTTGCCCTGTCTACTAACACAACCAAAGTGAAGGAGTTTGGAATT GACCCTCAAAACATGTTCGAGTTCTGGGATTGGGTGGGAGGACGCTACTCGCTGTGGTCGGCCATCGGA CTCTCCATTGCCCTGCACGTGGGTTTTGACAACTTCGAGCAGCTGCTCTCGGGGGCTCACTGGATGGAC CAGCACTTCCGCACGACGCCCCTGGAGAAGAACGCCCCCGTCTTGCTGGCCCTGCTGGGTATCTGGTAC ATCAACTGCTTTGGGTGTGAGACACACGCCATGCTGCCCTATGACCAGTACCTGCACCGCTTTGCTGCG TACTTCCAGCAGGGCGACATGGAGTCCAATGGGAAATACATCACCAAATCTGGAACCCGTGTGGACCAC CAGACAGGCCCCATTGTGTGGGGGGAGCCAGGGACCAATGGCCAGCATGCTTTTTACCAGCTCATCCAC CAAGGCACCAAGATGATACCCTGTGACTTCCTCATCCCGGTCCAGACCCAGCACCCCATACGGAAGGGT CTGCATCACAAGATCCTCCTGGCCAACTTCTTGGCCCAGACAGAGGCCCTGATGAGGGGAAAATCGACG GAGGAGGCCCGAAAGGAGCTCCAGGCTGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACAT AAGGTCTTTGAAGGAAATCGCCCAACCAACTCTATTGTGTTCACCAAGCTCACACCATTCATGCTTGGA GCCTTGGTCGCCATGTATGAGCACAAGATCTTCGTTCAGGGCATCATCTGGGACATCAACAGCTTTGAC CAGTGGGGAGTGGAGCTGGGAAAGCAGCTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAA GTGACCTCTCACGACGCTTCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTC CAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289790
Insert Size	1593 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289790.2
RefSeq Size	4132 bp
RefSeq ORF	1593 bp
Locus ID	2821
Protein Families	Druggable Genome
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism
MW	60.2 kDa
Gene Summary	This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (4) differs in the 5' UTR, uses a downstream start codon, and lacks an in-frame exon in the central coding region, compared to variant 3. The encoded isoform (4) is shorter, compared to isoform 3.

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