Complement factor 8 beta (C8B) (NM_001278544) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C82
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336631 representing NM_001278544. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACACTTGTATGACTCTGGCCTTCACTCTCTCTGGGCGTTTCTTCATGCTGCTTTCTCAGTACAGG TATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAA GACTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGA AGGTGTGTAAACCGCAGACTTCTTTGCAATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAAC TGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGT GGGATAAATTTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGC TCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACC CAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTTGAACGCAATGTCACAGAG AAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATACCTGGAATATTTGAACTTGGCATCAGT AGTCAAAGTGATCGAGGCAAACACTATATTAGGAGAACCAAACGATTCTCTCATACTAAAAGCGTATTT CTGCATGCACGCTCTGACCTTGAAGTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTAC GAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGT GATTTTGGGACCCACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATGAAC AAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAAAATGATTTTAAA ATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTAGGCAAATGCAGAGGTATTCTG AATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTGGAGGACTTGGTGGTCCTGGTACGAGGAGGG GCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGA GACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCC ACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAA GTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGCTGTGAC TGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAGAATACCCCCATTGAT GGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGACGTAAGACAAGACAAAGGCAGTGT AACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTGCTTCAGAAACACTTGACTGCTCC TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278544
Insert Size	1590 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278544.1
RefSeq Size	2307 bp
RefSeq ORF	1590 bp
Locus ID	732
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
MW	60.1 kDa
Gene Summary	This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1.

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