Complement factor 8 beta (C8B) (NM_001278544) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C82
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238737 representing NM_001278544 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACTTGTATGACTCTGGCCTTCACTCTCTCTGGGCGTTTCTTCATGCTGCTTTCTCAGTACAGGT ATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAAGA CTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGG TGTGTAAACCGCAGACTTCTTTGCAATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTA GAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGAT AAATTTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGCTCCCCG CATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACCCAAGGCA AATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGC AAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGT GATCGAGGCAAACACTATATTAGGAGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCAC GCTCTGACCTTGAAGTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCA GAGAGTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTTGGGACC CACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGG AGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCAT TGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGAC AGAAACAAGAGGGACACCATGGTGGAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCA CCACCCTGGCATACCAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAA CCCAGCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCCTATTCC AGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTG CTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATC CCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTCA AATTGGTCTTCATGCTCTGGAAGACGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATG GGGGTAGCCCCTGTTCAGGCCCTGCTTCAGAAACACTTGACTGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238737 representing NM_001278544 Red=Cloning site Green=Tags(s) MDTCMTLAFTLSGRFFMLLSQYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVRCEGFVCAQTGR CVNRRLLCNGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPVLDHRYYAGGCSP HYILNTRFRKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFKIPGIFELGISSQS DRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGT HYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKD RNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYS STVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWS NWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278544
ORF Size	1587 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278544.1, NP_001265473.1
RefSeq Size	2307 bp
RefSeq ORF	1590 bp
Locus ID	732
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
MW	60.6 kDa
Gene Summary	This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

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