SWAP70 (NM_001297714) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC321; SWAP-70
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336619 representing NM_001297714. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAGCTTGAAGGAGGAGCTGCTCAAAGCCATCTGGCACGCCTTCACCGCACTCGACCAGGACCAC AGCGGCAAGGTCTCCAAGTCCCAGCTCAAGGTCCTTTCCCATAACCTGTGCACGGTGCTGAAGGTTCCT CATGACCCAGTTGCCCTTGAAGAGCACTTCAGGGATGATGATGAGGGTCCAGTGTCCAACCAGGGCTAC ATGCCTTATTTAAACAGGTTCATTTTGGAAAAGATTGAATACCTGCTTAAGAAGCTTACAGAAGCTATG GGAGGAGGTTGGCAGCAAGAACAATTTGAACATTATAAAATCAACTTTGATGACAGTAAAAATGGCCTT TCTGCATGGGAACTTATTGAGCTTATTGGAAATGGACAGTTTAGCAAAGGCATGGACCGGCAGACTGTG TCTATGGCAATTAATGAAGTCTTTAATGAACTTATATTAGATGTGTTAAAGCAGGGTTACATGATGAAA AAGGGCCACAGACGGAAAAACTGGACTGAAAGATGGTTTGTACTAAAACCCAACATAATTTCTTACTAT GTGAGTGAGGATCTGAAGGATAAGAAAGGAGACATTCTCTTGGATGAAAATTGCTGTGTAGAGTCCTTG CCTGACAAAGATGGAAAGAAATGCCTTTTTCTCGTAAAATGTTTTGATAAGACTTTTGAAATCAGTGCT TCAGATAAGAAGAAGAAACAGGAGTGGATTCAAGCCATTCATTCTACTATTCATCTGTTGAAGCTGGGC AGCCCTCCACCACACAAAGAAGCCCGCCAGCGTCGGAAAGAACTCCGGAAGAAGCAGCTGGCTGAACAA GAGGAACTGGAGCGACAAATGAAGGAACTCCAGGCCGCCAACGAAAGCAAGCAGCAGGAGCTGGAGGCC GTGCGGAAGAAACTGGAGGAAGCAGCATCTCGTGCAGCAGAAGAGGAAAAGAAACGCCTTCAGACTCAA GTGGAACTTCAGGCCAGGTTCAGCACAGAGCTGGAAAGAGAGAAGCTTATCAGACAGCAGATGGAAGAA CAGGTTGCTCAAAAGTCCTCTGAACTGGAACAGTATTTACAGCGAGTACGGGAGCTGGAAGACATGTAC CTAAAGCTGCAGGAGGCTCTTGAAGATGAGAGACAGGCCCGGCAAGATGAAGAGACAGTGCGGAAGCTT CAGGCCAGGTTGTTGGAGGAAGAGTCTTCCAAGAGGGCTGAACTAGAAAAGTGGCACTTGGAGCAGCAG CAGGCCATTCAGACAACCGAGGCGGAGAAGCAGGAGTTGGAGAATCAGCGTGTCCTGAAGGAACAGGCC CTGCAGGAGGCCATGGAGCAGCTGGAGCAGCTTGAGTTAGAACGGAAGCAAGCACTTGAGCAGTACGAG GAAGTTAAAAAGAAGCTGGAGATGGCAACTAATAAGACCAAGAGCTGGAAGGACAAAGTGGCCCATCAT GAAGGATTAATTCGACTGATAGAACCAGGTTCAAAGAACCCTCACCTGATCACTAACTGGGGACCTGCA GCTTTCACTGAGGCAGAACTTGAAGAGAGAGAGAAGAACTGGAAAGAGAAAAAGACCACGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297714
Insert Size	1584 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297714.1
RefSeq Size	4708 bp
RefSeq ORF	1584 bp
Locus ID	23075
UniProt ID	Q9UH65
MW	62 kDa
Gene Summary	Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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