SWAP70 (NM_001297714) Human Tagged ORF Clone
CAT#: RC238725
- TrueORF®
SWAP70 (myc-DDK-tagged) - Human SWAP switching B-cell complex 70kDa subunit (SWAP70), transcript variant 2
ORF Plasmid: tGFP
"NM_001297714" in other vectors (2)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HSPC321; SWAP-70 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238725 representing NM_001297714
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCTTGAAGGAGGAGCTGCTCAAAGCCATCTGGCACGCCTTCACCGCACTCGACCAGGACCACA GCGGCAAGGTCTCCAAGTCCCAGCTCAAGGTCCTTTCCCATAACCTGTGCACGGTGCTGAAGGTTCCTCA TGACCCAGTTGCCCTTGAAGAGCACTTCAGGGATGATGATGAGGGTCCAGTGTCCAACCAGGGCTACATG CCTTATTTAAACAGGTTCATTTTGGAAAAGATTGAATACCTGCTTAAGAAGCTTACAGAAGCTATGGGAG GAGGTTGGCAGCAAGAACAATTTGAACATTATAAAATCAACTTTGATGACAGTAAAAATGGCCTTTCTGC ATGGGAACTTATTGAGCTTATTGGAAATGGACAGTTTAGCAAAGGCATGGACCGGCAGACTGTGTCTATG GCAATTAATGAAGTCTTTAATGAACTTATATTAGATGTGTTAAAGCAGGGTTACATGATGAAAAAGGGCC ACAGACGGAAAAACTGGACTGAAAGATGGTTTGTACTAAAACCCAACATAATTTCTTACTATGTGAGTGA GGATCTGAAGGATAAGAAAGGAGACATTCTCTTGGATGAAAATTGCTGTGTAGAGTCCTTGCCTGACAAA GATGGAAAGAAATGCCTTTTTCTCGTAAAATGTTTTGATAAGACTTTTGAAATCAGTGCTTCAGATAAGA AGAAGAAACAGGAGTGGATTCAAGCCATTCATTCTACTATTCATCTGTTGAAGCTGGGCAGCCCTCCACC ACACAAAGAAGCCCGCCAGCGTCGGAAAGAACTCCGGAAGAAGCAGCTGGCTGAACAAGAGGAACTGGAG CGACAAATGAAGGAACTCCAGGCCGCCAACGAAAGCAAGCAGCAGGAGCTGGAGGCCGTGCGGAAGAAAC TGGAGGAAGCAGCATCTCGTGCAGCAGAAGAGGAAAAGAAACGCCTTCAGACTCAAGTGGAACTTCAGGC CAGGTTCAGCACAGAGCTGGAAAGAGAGAAGCTTATCAGACAGCAGATGGAAGAACAGGTTGCTCAAAAG TCCTCTGAACTGGAACAGTATTTACAGCGAGTACGGGAGCTGGAAGACATGTACCTAAAGCTGCAGGAGG CTCTTGAAGATGAGAGACAGGCCCGGCAAGATGAAGAGACAGTGCGGAAGCTTCAGGCCAGGTTGTTGGA GGAAGAGTCTTCCAAGAGGGCTGAACTAGAAAAGTGGCACTTGGAGCAGCAGCAGGCCATTCAGACAACC GAGGCGGAGAAGCAGGAGTTGGAGAATCAGCGTGTCCTGAAGGAACAGGCCCTGCAGGAGGCCATGGAGC AGCTGGAGCAGCTTGAGTTAGAACGGAAGCAAGCACTTGAGCAGTACGAGGAAGTTAAAAAGAAGCTGGA GATGGCAACTAATAAGACCAAGAGCTGGAAGGACAAAGTGGCCCATCATGAAGGATTAATTCGACTGATA GAACCAGGTTCAAAGAACCCTCACCTGATCACTAACTGGGGACCTGCAGCTTTCACTGAGGCAGAACTTG AAGAGAGAGAGAAGAACTGGAAAGAGAAAAAGACCACGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238725 representing NM_001297714
Red=Cloning site Green=Tags(s) MGSLKEELLKAIWHAFTALDQDHSGKVSKSQLKVLSHNLCTVLKVPHDPVALEEHFRDDDEGPVSNQGYM PYLNRFILEKIEYLLKKLTEAMGGGWQQEQFEHYKINFDDSKNGLSAWELIELIGNGQFSKGMDRQTVSM AINEVFNELILDVLKQGYMMKKGHRRKNWTERWFVLKPNIISYYVSEDLKDKKGDILLDENCCVESLPDK DGKKCLFLVKCFDKTFEISASDKKKKQEWIQAIHSTIHLLKLGSPPPHKEARQRRKELRKKQLAEQEELE RQMKELQAANESKQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQK SSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQARLLEEESSKRAELEKWHLEQQQAIQTT EAEKQELENQRVLKEQALQEAMEQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLI EPGSKNPHLITNWGPAAFTEAELEEREKNWKEKKTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001297714 |
ORF Size | 1581 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297714.2 |
RefSeq Size | 4708 bp |
RefSeq ORF | 1584 bp |
Locus ID | 23075 |
UniProt ID | Q9UH65 |
MW | 62.5 kDa |
Gene Summary | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |