SWAP70 (NM_001297714) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HSPC321; SWAP-70
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238725 representing NM_001297714 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCTTGAAGGAGGAGCTGCTCAAAGCCATCTGGCACGCCTTCACCGCACTCGACCAGGACCACA GCGGCAAGGTCTCCAAGTCCCAGCTCAAGGTCCTTTCCCATAACCTGTGCACGGTGCTGAAGGTTCCTCA TGACCCAGTTGCCCTTGAAGAGCACTTCAGGGATGATGATGAGGGTCCAGTGTCCAACCAGGGCTACATG CCTTATTTAAACAGGTTCATTTTGGAAAAGATTGAATACCTGCTTAAGAAGCTTACAGAAGCTATGGGAG GAGGTTGGCAGCAAGAACAATTTGAACATTATAAAATCAACTTTGATGACAGTAAAAATGGCCTTTCTGC ATGGGAACTTATTGAGCTTATTGGAAATGGACAGTTTAGCAAAGGCATGGACCGGCAGACTGTGTCTATG GCAATTAATGAAGTCTTTAATGAACTTATATTAGATGTGTTAAAGCAGGGTTACATGATGAAAAAGGGCC ACAGACGGAAAAACTGGACTGAAAGATGGTTTGTACTAAAACCCAACATAATTTCTTACTATGTGAGTGA GGATCTGAAGGATAAGAAAGGAGACATTCTCTTGGATGAAAATTGCTGTGTAGAGTCCTTGCCTGACAAA GATGGAAAGAAATGCCTTTTTCTCGTAAAATGTTTTGATAAGACTTTTGAAATCAGTGCTTCAGATAAGA AGAAGAAACAGGAGTGGATTCAAGCCATTCATTCTACTATTCATCTGTTGAAGCTGGGCAGCCCTCCACC ACACAAAGAAGCCCGCCAGCGTCGGAAAGAACTCCGGAAGAAGCAGCTGGCTGAACAAGAGGAACTGGAG CGACAAATGAAGGAACTCCAGGCCGCCAACGAAAGCAAGCAGCAGGAGCTGGAGGCCGTGCGGAAGAAAC TGGAGGAAGCAGCATCTCGTGCAGCAGAAGAGGAAAAGAAACGCCTTCAGACTCAAGTGGAACTTCAGGC CAGGTTCAGCACAGAGCTGGAAAGAGAGAAGCTTATCAGACAGCAGATGGAAGAACAGGTTGCTCAAAAG TCCTCTGAACTGGAACAGTATTTACAGCGAGTACGGGAGCTGGAAGACATGTACCTAAAGCTGCAGGAGG CTCTTGAAGATGAGAGACAGGCCCGGCAAGATGAAGAGACAGTGCGGAAGCTTCAGGCCAGGTTGTTGGA GGAAGAGTCTTCCAAGAGGGCTGAACTAGAAAAGTGGCACTTGGAGCAGCAGCAGGCCATTCAGACAACC GAGGCGGAGAAGCAGGAGTTGGAGAATCAGCGTGTCCTGAAGGAACAGGCCCTGCAGGAGGCCATGGAGC AGCTGGAGCAGCTTGAGTTAGAACGGAAGCAAGCACTTGAGCAGTACGAGGAAGTTAAAAAGAAGCTGGA GATGGCAACTAATAAGACCAAGAGCTGGAAGGACAAAGTGGCCCATCATGAAGGATTAATTCGACTGATA GAACCAGGTTCAAAGAACCCTCACCTGATCACTAACTGGGGACCTGCAGCTTTCACTGAGGCAGAACTTG AAGAGAGAGAGAAGAACTGGAAAGAGAAAAAGACCACGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238725 representing NM_001297714 Red=Cloning site Green=Tags(s) MGSLKEELLKAIWHAFTALDQDHSGKVSKSQLKVLSHNLCTVLKVPHDPVALEEHFRDDDEGPVSNQGYM PYLNRFILEKIEYLLKKLTEAMGGGWQQEQFEHYKINFDDSKNGLSAWELIELIGNGQFSKGMDRQTVSM AINEVFNELILDVLKQGYMMKKGHRRKNWTERWFVLKPNIISYYVSEDLKDKKGDILLDENCCVESLPDK DGKKCLFLVKCFDKTFEISASDKKKKQEWIQAIHSTIHLLKLGSPPPHKEARQRRKELRKKQLAEQEELE RQMKELQAANESKQQELEAVRKKLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQK SSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQARLLEEESSKRAELEKWHLEQQQAIQTT EAEKQELENQRVLKEQALQEAMEQLEQLELERKQALEQYEEVKKKLEMATNKTKSWKDKVAHHEGLIRLI EPGSKNPHLITNWGPAAFTEAELEEREKNWKEKKTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297714
ORF Size	1581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297714.2
RefSeq Size	4708 bp
RefSeq ORF	1584 bp
Locus ID	23075
UniProt ID	Q9UH65
MW	62.5 kDa
Gene Summary	Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).[UniProtKB/Swiss-Prot Function]

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