MIRO1 (RHOT1) (NM_001288758) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARHT1; MIRO-1; MIRO1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336606 representing NM_001288758. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAA AACCTGAAGAACATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTG TACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAAGCCCTTACTCGTATATTTAAAATATCT GATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACT CCATTAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCT GACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTTACACACACTTTTTATCCAGAGAGGGAGACACGAA ACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTGTTC CCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGC ACCTTTGACAAGCATGATTTGGATAGAGACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAA GTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAGAGGCTGGATA ACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATAT TTGGGCTATCTAGGCTATTCAATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGA GATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGATGTAATGTAATTGGAGTG AAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATT CGTGAAGATCATAAATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTG TTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATCATTTGTGATGTTGTATGCCTGGTA TATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGC AGAATACCTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCA CCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACCACAAGCCTTCACTTGCAATACTGCTGATGCC CCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCAGAGGATCATTACAGAGACAGA CTCTCCCGAGACATGGGCCACACTGATAGAATAGAGAATTTGAGAAAAATCTGGGTCTTTCTAAAAACT GCTTTGCACGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACT GTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTATTGAAACAGCGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288758
Insert Size	1572 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288758.1
RefSeq Size	3360 bp
RefSeq ORF	1572 bp
Locus ID	55288
UniProt ID	Q8IXI2
Protein Families	Transmembrane
MW	60.4 kDa
Gene Summary	Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) differs in the 5' UTR, contains multiple coding region differences and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 7, which is shorter than isoform 1.

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