INPP5F (NM_001243194) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hSAC2; MSTP007; MSTPO47; SAC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336600 representing NM_001243194. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGTCATGACGTAATTTTTATGGCTTGGCTCAAGCAACAATTTTCAGAGTGCACCCTCATTGATGCT ACTCACAGAGACGTGGATGTGCTGTTACTGCTTTCTAACTCTGCCTACTACGTGGCCTATTATGATGAT GAAGTTGATAAAGTAAACCAGTATCAACGACTAAGTCTAGAAAACCTGGAAAAAATTGAAATAGGCCCT GAACCCACTCTTTTTGGTAAGCCAAAGTTCTCCTGCATGCGACTGCACTACAGATACAAAGAAGCGAGT GGCTATTTCCACACATTGCGAGCTGTAATGCGTAATCCTGAAGAGGATGGAAAAGATACCCTTCAGTGC ATTGCAGAGATGCTGCAGATCACCAAGCAAGCCATGGGATCGGATTTACCCATAATTGAGAAGAAACTT GAGAGGAAGAGCAGTAAACCTCACGAAGACATCATTGGTATCAGGTCTCAAAACCAAGGTTCTTTGGCC CAGGGAAAGAATTTTTTAATGAGCAAATTTTCATCTCTAAATCAAAAAGTGAAGCAGACCAAATCCAAT GTAAATATTGGCAACCTCCGAAAGCTAGGAAACTTTACCAAACCTGAAATGAAAGTTAACTTTCTAAAA CCAAACTTAAAAGTAAATCTTTGGAAATCAGATAGTAGTCTTGAAACTATGGAAAACACAGGAGTGATG GATAAGGTTCAGGCAGAGTCTGATGGGGACATGTCTTCAGATAATGACTCATACCACTCTGATGAATTC CTTACAAATTCTAAGTCTGATGAAGACAGGCAGCTAGCTAACTCATTAGAGAGTGTAGGGCCAATAGAT TACGTTCTTCCTAGTTGTGGTATTATTGCCTCAGCGCCTCGATTGGGCAGTCGGTCCCAGTCTCTTAGC AGCACAGATAGTAGCGTTCATGCTCCTTCAGAGATTACTGTTGCTCATGGGAGTGGGCTTGGAAAAGGC CAGGAGTCTCCTTTGAAGAAAAGTCCTTCTGCTGGCGACGTACACATATTGACTGGCTTTGCCAAGCCT ATGGATATTTACTGCCACAGATTTGTGCAAGATGCACAGAACAAAGTGACCCACCTATCAGAGACCAGA TCTGTGTCTCAGCAGGCTAGTCAGGAAAGAAATCAAATGACCAATCAAGTTTCAAATGAAACCCAATCA GAATCAACAGAACAGACACCTTCTCGGCCATCGCAATTAGATGTCTCTCTTTCTGCAACAGGCCCACAG TTTTTGTCAGTTGAGCCAGCGCATTCAGTTGCATCTCAAAAAACCCCCACCTCCGCTTCCAGCATGCTT GAACTTGAGACAGGGCTTCATGTAACTCCTTCTCCTTCAGAGAGCAGTAGCAGCAGAGCAGTCTCTCCC TTTGCCAAGATTCGAAGTTCCATGGTCCAGGTTGCTAGTATTACCCAAGCTGGATTAACCCATGGGATA AACTTTGCAGTGTCAAAAGTTCAGAAGAGTCCTCCAGAACCTGAAATCATTAATCAAGTCCAGCAAAAT GAACTTAAAAAGATGTTTATACAATGCCAGACACGGATAATTCAGATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001243194
Insert Size	1569 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243194.1
RefSeq Size	3167 bp
RefSeq ORF	1569 bp
Locus ID	22876
UniProt ID	Q9Y2H2
Protein Families	Druggable Genome
MW	57.8 kDa
Gene Summary	The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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