RBPSUHL (RBPJL) (NM_001281449) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RBPL; RBPSUHL; SUHL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336573 representing NM_001281449. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCCCGCAGGGGCAGCAGACCCCTCAGTGCCTCCCAATCCTTTGACTCACCTGAGCCTGCAGGAC AGATCAGAGATGCAGCTGCAGAGCGAAGCCGACAGGCGGAGCCTCCCGGGCACTTGGACCAGGTCATCC CCAGAGCACACCACCATTCTGAGGGGAGGCGTGCGCAGGTGCCTGCAGCAACAGTGTGAACAGACTGTG CGGATCCTGCATGCCAAGGTGGCCCAGAAATCATACGGAAATGAGAAGCGGTTCTTCTGCCCCCCGCCC TGTGTCTACCTCTCGGGGCCTGGCTGGAGGGTGAAGCCAGGGCAGGATCAAGCTCACCAGGCGGGGGAA ACGGGGCCCACGGTCTGCGGTTACATGGGACTGGACAGCGCGTCCGGCAGCGCCACTGAGACGCAGAAG CTGAATTTCGAGCAGCAGCCGGACTCCAGGGAATTCGGCTGCGCCAAGACCCTGTACATCTCAGATGCA GACAAGAGGAAGCACTTTCGGCTGGTGCTGCGGCTGGTGCTGCGCGGGGGCCGGGAGCTGGGTACCTTC CACAGCCGCCTTATCAAGGTCATCTCGAAGCCCTCGCAGAAGAAGCAGTCGCTGAAAAACACCGATCTG TGCATATCCTCCGGCTCAAAGGTCTCCCTCTTCAACCGCCTGCGCTCTCAGACGGTCTCCACACGCTAC CTCTCTGTGGAGGATGGGGCCTTTGTGGCCAGTGCACGACAGTGGGCTGCCTTCACGCTCCACCTGGCT GATGGGCACTCTGCCCAAGGAGACTTCCCACCGCGAGAGGGCTACGTTCGCTATGGCTCCCTGGTGCAG CTCGTCTGCACGGTCACCGGCATCACACTACCTCCCATGATCATCCGTAAAGTAGCAAAACAGTGTGCG CTCCTTGATGTGGATGAGCCCATCTCCCAGCTGCACAAGTGTGCATTCCAGTTTCCAGGCAGTCCCCCA GGAGGGGGTGGCACCTACTTATGCCTTGCCACAGAGAAGGTGGTGCAATTTCAGGCCTCTCCCTGCCCC AAGGAGGCGAACAGGGCTCTGCTTAACGACAGCTCTTGCTGGACCATCATCGGCACCGAGTCGGTGGAA TTTTCCTTCAGCACCAGCCTGGCGTGTACCCTGGAGCCGGTCACTCCGGTGCCTCTCATCAGCACCCTA GAGCTGAGCGGCGGGGGCGACGTGGCCACGCTGGAGCTCCACGGAGAGAACTTCCACGCGGGGCTCAAG GTGTGGTTTGGGGACGTGGAGGCAGAAACCATGTACAGCCCGCGGTCCCTGGTGTGCGTGGTGCCGGAC GTGGCGGCCTTCTGCAGCGACTGGCGCTGGCTGCGCGCTCCCATCACAATCCCCATGAGCCTGGTGCGC GCCGACGGGCTCTTCTACCCTAGTGCCTTCTCCTTCACCTACACCCCGGAATACAGCGTGCGGCCGGGT CACCCCGGCGTCCCCGAGCCCGCCACCGACGCCGACGCGCTCCTGGAGAGCATCCATCAGGAGTTCACG CGCACCAACTTCCACCTCTTCATCCAGACTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281449
Insert Size	1551 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281449.1
RefSeq Size	2496 bp
RefSeq ORF	1551 bp
Locus ID	11317
UniProt ID	Q9UBG7
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Notch signaling pathway
MW	56.6 kDa
Gene Summary	This gene encodes a member of the suppressor of hairless protein family. A similar protein in mouse is a transcription factor that binds to DNA sequences almost identical to that bound by the Notch receptor signaling pathway transcription factor recombining binding protein J. The mouse protein has been shown to activate transcription in concert with Epstein-Barr virus nuclear antigen-2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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