SPTLC1 (NM_001281303) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336549 representing NM_001281303. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCTCCTGCTTAC CATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTCTCTAAGACTTACAAATTA CAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTGATTGAAGAGTGGCAACCAGAACCTCTT GTTCCTCCTGTCCCAAAAGACCATCCTGCTCTCAACTACAACATCGTTTCAGGCCCTCCAAGCCACAAA ACTGTGGTGAATGGAAAAGAATGTATAAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCT AGGGTTAAGGCAGCAGCTTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTT TATGGCACATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAAGCC ATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAAAGAGGGGACATT GTTTTTGTAGATAGAGCTGCCTGCTTTGCTATTCAGAAAGGATTACAGGCATCCCGTAGTGACATTAAG TTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTACTAAAAGAACAAGAGATCGAAGATCAAAAG AATCCTCGCAAGGCTCGTGTAACTCGGCGTTTCATTGTAGTAGAAGGATTGTATATGAATACTGGAACT ATTTGTCCTCTTCCAGAATTGGTTAAGTTAAAATACAAATACAAAGCAAGAATCTTCCTGGAGGAAAGC CTTTCATTTGGAGTCCTAGGAGAGCATGGCCGAGGAGTCACTGAACACTATGGAATCAATATTGATGAT ATTGATCTTATCAGTGCCAACATGGAGAATGCACTTGCTTCTATTGGAGGTTTCTGCTGTGGCAGGTCT TTTGTAATTGACCATCAGCGACTTTCCGGCCAGGGATACTGCTTTTCAGCTTCGTTACCTCCCCTGTTA GCTGCTGCAGCAATTGAGGCCCTCAACATCATGGAAGAGAATCCAGGTATTTTTGCAGTGTTGAAGGAA AAGTGCGGACAAATTCATAAAGCTTTACAAGGCATTTCTGGATTAAAAGTGGTGGGGGAGTCCCTTTCT CCAGCCTTTCACCTACAACTGGAAGAGAGCACTGGGTCTCGCGAGCAAGATGTCAGACTGCTTCAGGAA ATTGTAGATCAATGCATGAACAGAAGTATTGCATTAACTCAGGCGCGCTACTTGGAGAAAGAAGAGAAG TGTCTCCCTCCTCCCAGAGGAAGAACTGGAGAGAGCTGCGTCCACCATCAAGGAGGTAGCCCAGGCCGT CCTGCTCTAGGCAGAGTCCCGGGACCATGGCCTCCTGCCACACAACACGCAGAGAGGACTCAAGACTCC CGCTGGCCATGGAGTGGCCTGAAAGAGAGCAAGAACATGTGGATCTTTGATAGGATTGTTACCAAATGG TGTCAGTATGGACCAATTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281303
Insert Size	1542 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281303.1
RefSeq Size	2833 bp
RefSeq ORF	1542 bp
Locus ID	10558
UniProt ID	O15269
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Metabolic pathways, Sphingolipid metabolism
MW	57.4 kDa
Gene Summary	This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) uses an alternate splice site in the 3'-terminal exon, which results in a translational frameshift, compared to variant 1. The encoded isoform (c) has a longer and distinct C-terminus, compared to isoform a.

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