ATE1 (NM_001288736) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336537 representing NM_001288736. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGTCGGTGGTGGAATACAAGGGCCTCAGGGCCGGCTACCACTGCGGCTACTGCGATTCCAAGGAG GGCAAAGCGTCCTGCGGCATGTGGGCACATTCCATGACAGTACAGGATTATCAGGATCTCATAGACCGA GGATGGCGAAGAAGTGGAAAATATGTGTACAAACCTGTCATGAATCAAACATGTTGTCCTCAGTACACA ATAAGGTGCCGACCTTTACAATTTCAGCCTTCAAAATCTCACAAGAAGGTTTTGAAAAAAATGTTGAAA TTTCTAGCTAAAGGGGAGGTTCCCAAAGGAAGTTGTGAGGATGAGCCCATGGATTCCACAATGGATGAT GCTGTTGCGGGTGACTTTGCATTGATAAATAAACTGGATATACAGTGTGATCTTAAAACACTCAGTGAT GACATCAAAGAGAGTTTAGAGAGTGAAGGAAAAAATTCAAAGAAAGAAGAACCTCAGGAATTACTTCAG TCACAAGATTTCGTAGGAGAGAAGTTGGGCTCTGGTGAACCGTCACATTCAGTTAAAGTTCACACAGTT CCTAAGCCAGGCAAAGGGGCTGATTTGAGTAAGCCTCCATGTCGAAAAGCAAAGGAAATCCGGAAAGAA AGGAAAAGGTTAAAACTAATGCAGCAGAACCCAGCTGGAGAACTTGAGGGTTTCCAGGCTCAAGGTCAC CCACCATCTTTGTTTCCACCAAAGGCTAAATCCAACCAGCCAAAATCACTCGAAGATTTAATTTTTGAG TCTTTACCAGAGAATGCATCACACAAGTTAGAGGTGAGGTTAGTACCTGTCTCCTTTGAGGACCCAGAG TTCAAGTCGTCTTTCAGCCAGTCCTTTTCTTTGTATGTCAAGTATCAAGTGGCCATACACCAGGATCCA CCCGATGAATGTGGGAAGACTGAGTTCACAAGATTCCTTTGCAGTTCACCCTTGGAGGCAGAGACTCCC CCTAATGGGCCAGATTGTGGCTATGGCTCCTTTCACCAGCAGTACTGGCTTGACGGAAAGATCATTGCT GTGGGGGTGATTGACATCCTCCCAAACTGTGTATCATCTGTGTATTTGTACTACGATCCTGATTATTCG TTTTTGTCTTTGGGCGTCTACTCTGCACTACGAGAAATTGCTTTTACTAGGCAGCTTCATGAGAAAACT TCTCAACTCAGCTATTATTATATGGGTTTCTACATTCATTCATGTCCCAAGATGAAATATAAGGGTCAG TATAGACCTTCTGATTTGCTGTGCCCTGAGACATATGTTTGGGTACCCATTGAGCAATGCCTGCCTTCA CTTGAAAACTCCAAGTACTGCCGTTTCAACCAGGACCCAGAAGCAGTGGATGAGGATCGCAGTACGGAA CCTGACCGATTGCAGGTGTTTCACAAGAGAGCCATCATGCCTTACGGTGTTTATAAGAAACAGCAGAAA GACCCAAGTGAGGAGGCTGCTGTTCTGCAGTACGCCAGCCTGGTGGGGCAGAAGTGCTCCGAGCGGATG CTGCTGTTCAGAAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288736
Insert Size	1536 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288736.1
RefSeq Size	5165 bp
RefSeq ORF	1536 bp
Locus ID	11101
UniProt ID	O95260
MW	58.1 kDa
Gene Summary	This gene encodes an arginyltransferase, an enzyme that is involved in posttranslational conjugation of arginine to N-terminal aspartate or glutamate residues. Conjugation of arginine to the N-terminal aspartate or glutamate targets proteins for ubiquitin-dependent degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (5) differs in the 5' UTR and coding region, and uses an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (5) has a shorter and distinct N-terminus and a unique internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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