ATE1 (NM_001288736) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238643 representing NM_001288736 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGTGGTGGAATACAAGGGCCTCAGGGCCGGCTACCACTGCGGCTACTGCGATTCCAAGGAGG GCAAAGCGTCCTGCGGCATGTGGGCACATTCCATGACAGTACAGGATTATCAGGATCTCATAGACCGAGG ATGGCGAAGAAGTGGAAAATATGTGTACAAACCTGTCATGAATCAAACATGTTGTCCTCAGTACACAATA AGGTGCCGACCTTTACAATTTCAGCCTTCAAAATCTCACAAGAAGGTTTTGAAAAAAATGTTGAAATTTC TAGCTAAAGGGGAGGTTCCCAAAGGAAGTTGTGAGGATGAGCCCATGGATTCCACAATGGATGATGCTGT TGCGGGTGACTTTGCATTGATAAATAAACTGGATATACAGTGTGATCTTAAAACACTCAGTGATGACATC AAAGAGAGTTTAGAGAGTGAAGGAAAAAATTCAAAGAAAGAAGAACCTCAGGAATTACTTCAGTCACAAG ATTTCGTAGGAGAGAAGTTGGGCTCTGGTGAACCGTCACATTCAGTTAAAGTTCACACAGTTCCTAAGCC AGGCAAAGGGGCTGATTTGAGTAAGCCTCCATGTCGAAAAGCAAAGGAAATCCGGAAAGAAAGGAAAAGG TTAAAACTAATGCAGCAGAACCCAGCTGGAGAACTTGAGGGTTTCCAGGCTCAAGGTCACCCACCATCTT TGTTTCCACCAAAGGCTAAATCCAACCAGCCAAAATCACTCGAAGATTTAATTTTTGAGTCTTTACCAGA GAATGCATCACACAAGTTAGAGGTGAGGTTAGTACCTGTCTCCTTTGAGGACCCAGAGTTCAAGTCGTCT TTCAGCCAGTCCTTTTCTTTGTATGTCAAGTATCAAGTGGCCATACACCAGGATCCACCCGATGAATGTG GGAAGACTGAGTTCACAAGATTCCTTTGCAGTTCACCCTTGGAGGCAGAGACTCCCCCTAATGGGCCAGA TTGTGGCTATGGCTCCTTTCACCAGCAGTACTGGCTTGACGGAAAGATCATTGCTGTGGGGGTGATTGAC ATCCTCCCAAACTGTGTATCATCTGTGTATTTGTACTACGATCCTGATTATTCGTTTTTGTCTTTGGGCG TCTACTCTGCACTACGAGAAATTGCTTTTACTAGGCAGCTTCATGAGAAAACTTCTCAACTCAGCTATTA TTATATGGGTTTCTACATTCATTCATGTCCCAAGATGAAATATAAGGGTCAGTATAGACCTTCTGATTTG CTGTGCCCTGAGACATATGTTTGGGTACCCATTGAGCAATGCCTGCCTTCACTTGAAAACTCCAAGTACT GCCGTTTCAACCAGGACCCAGAAGCAGTGGATGAGGATCGCAGTACGGAACCTGACCGATTGCAGGTGTT TCACAAGAGAGCCATCATGCCTTACGGTGTTTATAAGAAACAGCAGAAAGACCCAAGTGAGGAGGCTGCT GTTCTGCAGTACGCCAGCCTGGTGGGGCAGAAGTGCTCCGAGCGGATGCTGCTGTTCAGAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238643 representing NM_001288736 Red=Cloning site Green=Tags(s) MASVVEYKGLRAGYHCGYCDSKEGKASCGMWAHSMTVQDYQDLIDRGWRRSGKYVYKPVMNQTCCPQYTI RCRPLQFQPSKSHKKVLKKMLKFLAKGEVPKGSCEDEPMDSTMDDAVAGDFALINKLDIQCDLKTLSDDI KESLESEGKNSKKEEPQELLQSQDFVGEKLGSGEPSHSVKVHTVPKPGKGADLSKPPCRKAKEIRKERKR LKLMQQNPAGELEGFQAQGHPPSLFPPKAKSNQPKSLEDLIFESLPENASHKLEVRLVPVSFEDPEFKSS FSQSFSLYVKYQVAIHQDPPDECGKTEFTRFLCSSPLEAETPPNGPDCGYGSFHQQYWLDGKIIAVGVID ILPNCVSSVYLYYDPDYSFLSLGVYSALREIAFTRQLHEKTSQLSYYYMGFYIHSCPKMKYKGQYRPSDL LCPETYVWVPIEQCLPSLENSKYCRFNQDPEAVDEDRSTEPDRLQVFHKRAIMPYGVYKKQQKDPSEEAA VLQYASLVGQKCSERMLLFRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288736
ORF Size	1533 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288736.1, NP_001275665.1
RefSeq Size	5165 bp
RefSeq ORF	1536 bp
Locus ID	11101
UniProt ID	O95260
MW	58.6 kDa
Gene Summary	This gene encodes an arginyltransferase, an enzyme that is involved in posttranslational conjugation of arginine to N-terminal aspartate or glutamate residues. Conjugation of arginine to the N-terminal aspartate or glutamate targets proteins for ubiquitin-dependent degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

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