SPATA18 (NM_001297608) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Mieap; SPETEX1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336507 representing NM_001297608. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAAAACCTGAAAAGACTGGTCTCAAACGAAACTTTACGAACGTTGCAGGAAAAGCTAGACTTC TGGCTGAAGGAGTACAACACAAACACGTGTGATCAAAATCTAAACCATTGCCTTGAACTCATTGAGCAA GTTGCCAAGGTGCAGGGACAACTCTTTGGGATCCTCACAGCAGCAGCCCAAGAAGGAGGACGTAATGAT GGTGTGGAAACAATCAAGTCACGCCTTTTGCCTTGGCTGGAGGCTTCCTTTACTGCTGCTTCCCTGGGA AAATCTGTTGACAGCAAGGTCCCCTCTCTGCAGGACACGTTTGATAGGGAGAGACATAAAGATCCCAGT CCTCGGGATCGGGATATGCAACAGTTAGACTCTAATTTGAACTCAACCCGGAGTCAATGCAACCAGGTT CAAGACGAGCTTAAATCTCTTCAAGCTCAGGAGGATGCCCGCCACAGAAACACAGATCAGAGGAGCTCA GAGAATAGGCGGTCAGAGCCTTGGAGCTTGGAGGAGCGGAAGCGTGAGCAGTGGAACTCACTCAAGCAG AATGCAGACCAGCAGGACACAGAAGCCATGTCCGATTATAAGAAACAGCTCCGAAACCTGAAGGAGGAG ATAGCTGTTCTGTCTGCTGAGAAAAGTGCACTCCAAGGAAGGTCCTCCAGGAGCCGGTCTCCCAGCCCT GCCCCTCGCAGCCGTAGCTGCAGCCGCAGCAGATCTGCCAGCCCCTCCACCGCTGTCAAGGTCAGGAGA CCGTCCCCAAACCGCTCCAAGCTGTCCAATGTGGCGCGCAAGGCTGCCCTCTTGTCCCGGTTCAGCGAT TCCTATTCCCAGGCCCGCCTGGACGCGCAGTGCCTGCTGCGGCGCTGCATCGACAAGGCTGAGACCGTT CAGCGGATCATCTACATCGCCACAGTGGAGGCATTCCATGTAGCAAAAATGGCATTCAGACACTTCAAG ATCCATGTGAGAAAATCGTTGACACCATCTTATGTGGGGTCGAATGACTTTGAGAATGCTGTCTTGGAT TATGTCATTTGTCATCTTGATCTATATGATTCTCAAAGCAGTGTCAATGATGTGATCCGAGCCATGAAT GTCAATCCCAAGATTTCATTCCCTCCTGTCGTTGACTTTTGCCTTCTCAGTGACTTCATCCAGGAGATA TGTTGCATTGCCTTTGCAATGCAGGCCTTAGAACCACCCCTAGATATTGCATATGGAGCAGATGGAGAA GTTTTTAATGATTGCAAATACCGCCGCAGCTACGACTCGGATTTCACTGCTCCCTTAGTCCTCTATCAC GTGTGGCCTGCTCTCATGGAGAATGACTGTGTCATTATGAAGGGAGAAGCTGTCACCAGGAGAGGGGCT TTTTGGAATTCGGTGCGATCTGTAAGTCGTTGTCGAAGCAGGAGTTTAAGTCCCATTTGCCCCCGTAGC CAAATTGGTTTAAACACGATGTCTCGAAGTCGGAGTCCTTCTCCAATAAGATGTGGATTGCCAAGATTT TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297608
Insert Size	1521 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297608.1
RefSeq Size	4329 bp
RefSeq ORF	1521 bp
Locus ID	132671
UniProt ID	Q8TC71
MW	57.5 kDa
Gene Summary	This gene encodes a p53-inducible protein that is able to induce lysosome-like organelles within mitochondria that eliminate oxidized mitochondrial proteins, thereby contributing to mitochondrial quality control. Dysregulation of mitochondrial quality control is associated with cancer and degenerative diseases. The encoded protein mediates accumulation of the lysosome-like mitochondrial organelles through interaction with B cell lymphoma 2 interacting protein 3 and B cell lymphoma 2 interacting protein 3 like at the outer mitochondrial membrane, which allows translocation of lysosomal proteins to the mitochondrial matrix from the cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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