PIP5K1 beta (PIP5K1B) (NM_001278253) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MSS4; STM7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336486 representing NM_001278253. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAAACAAAATGAAGAAAAAACCTATAAAAAG ACTGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACAGTGGGTAATCTCACTTCC AAGCCAGAACGAGATGTTCTTATGCAAGACTTTTATGTGGTGGAAAGTGTGTTCCTACCCAGCGAAGGG AGCAATCTGACCCCAGCACATCACTACCCAGACTTTAGATTTAAGACATACGCTCCATTAGCATTCCGA TATTTCAGAGAACTTTTTGGTATCAAGCCTGATGATTACTTGTATTCCATCTGCAGTGAACCTCTAATA GAACTGTCTAACCCTGGAGCCAGTGGATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAA ACAGTTCAGCACAAAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATGAATTTAAACCAG AATCCAAGGACTCTTTTGCCAAAATTTTACGGACTGTATTGTATGCAATCAGGAGGCATTAATATCAGG ATTGTGGTGATGAACAACGTTTTGCCACGCTCCATGAGAATGCACTTTACATATGACTTGAAAGGCTCA ACGTATAAGCGAAGAGCATCCCGTAAAGAGAGAGAGAAATCCAACCCCACATTTAAGGACTTAGATTTC CTGCAAGACATGCACGAAGGGTTGTATTTTGATACGGAAACATACAACGCGCTTATGAAAACACTTCAG AGAGACTGCCGGGTGCTAGAAAGCTTCAAGATCATGGATTATAGCCTTCTGTTGGGAATTCATTTCCTG GACCATTCCCTCAAAGAGAAAGAGGAGGAGACCCCACAAAATGTGCCTGATGCTAAGCGGACTGGGATG CAGAAGGTTCTCTACTCAACAGCCATGGAATCTATCCAGGGTCCAGGGAAATCTGGAGATGGGATAATC ACAGAGAACCCAGACACAATGGGAGGCATTCCAGCTAAAAGCCATAGGGGAGAAAAACTACTTTTATTT ATGGGCATTATTGACATTCTGCAATCATATAGGTTAATGAAGAAGTTAGAACATTCCTGGAAAGCTCTT GTTTATGATGGGGACACTGTTTCTGTTCATAGACCAAGCTTTTATGCAGACAGATTTCTTAAGTTCATG AATTCCAGAGTTTTCAAGAAAATTCAAGCTTTGAAGGCTTCACCGTCTAAGAAACGGTGCAATTCAATC GCCGCCCTAAAGGCCACTTCACAGGAGATTGTGTCCTCAATTAGCCAGGAATGGAAGGATGAGAAGCGG GATTTGCTGACTGAAGGACAAAGTTTTAGCAGCCTTGATGAAGAAGCCCTGGGATCCCGACACAGGCCA GACCTGGTCCCTAGCACTCCATCACTGTTTGAAGCTGCTTCCTTGGCAACCACAATTTCATCTTCTTCC TTATACGTCAATGAGCACTATCCACACGACAGGCCTACACTCTATTCAAACAGTAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278253
Insert Size	1509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278253.1
RefSeq Size	2682 bp
RefSeq ORF	1509 bp
Locus ID	8395
UniProt ID	O14986
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
MW	57 kDa
Gene Summary	Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon in the 3' end, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 2.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.