PAPOLA (NM_001293632) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PAP; PAP-alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336452 representing NM_001293632. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTAGTAGCAAGAACTTGCCAGCTTTATCCAAATGCAATAGCATCAACTCTTGTACATAAATTTTTC TTGGTATTTTCTAAATGGGAATGGCCAAATCCAGTGCTATTGAAACAGCCTGAAGAATGCAATCTTAAT TTGCCTGTATGGGACCCAAGGGTAAACCCCAGTGATAGGTACCATCTTATGCCTATAATTACACCAGCA TACCCACAACAGAACTCCACGTACAATGTGTCCGTTTCAACACGGATGGTCATGGTTGAGGAGTTTAAA CAAGGTCTTGCTATCACAGATGAAATTTTGCTGAGTAAGGCAGAGTGGTCCAAACTTTTTGAAGCTCCA AACTTCTTTCAAAAGTACAAGCATTATATTGTACTTCTAGCAAGTGCACCAACAGAAAAACAACGCCTG GAATGGGTGGGCTTGGTGGAATCAAAAATCCGAATCCTGGTTGGAAGCTTGGAGAAGAATGAATTTATT ACACTGGCTCATGTGAATCCCCAGTCATTTCCAGCACCCAAAGAAAATCCCGACAAGGAAGAATTTCGC ACGATGTGGGTGATTGGGTTAGTGTTTAAAAAAACAGAAAACTCTGAAAACCTCAGTGTTGATCTCACC TATGATATTCAGTCTTTCACAGATACAGTTTATAGGCAAGCAATAAACAGCAAGATGTTTGAGGTGGAT ATGAAAATTGCTGCAATGCATGTAAAAAGAAAGCAACTCCATCAACTACTACCTAATCATGTGCTTCAG AAAAAGAAAAAGCATTCAACAGAAGGTGTCAAATTGACAGCTCTCAATGACAGCAGCCTCGACTTGTCT ATGGACAGTGATAACAGCATGTCTGTGCCTTCACCTACTAGTGCTACGAAGACCAGTCCATTGAACAGT TCTGGCAGCTCTCAGGGCAGAAACAGTCCTGCTCCAGCTGTAACAGCAGCATCTGTGACCAACATACAG GCTACTGAAGTTTCTGTGCCACAAGTAAATTCCAGTGAAAGCTCAGGGGGTACATCGAGTGAAAGCATT CCTCAAACTGCCACACAACCAGCCATTTCTCCACCACCAAAGCCTACGGTCTCCAGAGTTGTTTCTTCA ACACGTCTGGTAAACCCACCACCTAGATCTTCAGGAAATGCAGCAACTTCAGGAAATGCAGCAACAAAA ATACCTACTCCTATAGTAGGAGTCAAGAGGACATCCTCACCTCATAAAGAAGAGAGTCCCAAGAAAACC AAAACAGAAGAGGATGAAACAAGTGAAGATGCTAACTGTCTTGCTTTGAGTGGACATGATAAAACAGAA GCAAAGGAACAACTTGATACAGAGACAAGTACAACTCAATCAGAAACTATTCAGACAGCGGCTTCTCTG TTGGCCTCTCAGAAAACATCCAGTACAGACCTTTCTGATATCCCTGCTCTCCCTGCAAATCCTATTCCT GTTATCAAGAATTCAATAAAACTGAGATTGAATCGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001293632
Insert Size	1488 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293632.1
RefSeq Size	4378 bp
RefSeq ORF	1488 bp
Locus ID	10914
UniProt ID	P51003
Protein Families	Transcription Factors
Protein Pathways	RNA degradation
MW	54.4 kDa
Gene Summary	The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (6) lacks two consecutive exons in the 5' region, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (5) has a shorter N-terminus, compared to isoform 1. Both variants 5 and 6 encode the same isoform 5.

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