LILRB2 (NM_001278406) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD85D; ILT-4; ILT4; LIR-2; LIR2; MIR-10; MIR10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336221 representing NM_001278406. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCCCCATCGTCACAGTCCTGATCTGTCTCGGGCTGAGTCTGGGCCCCAGGACCCGCGTGCAGACA GGGACCATCCCCAAGCCCACCCTGTGGGCTGAGCCAGACTCTGTGATCACCCAGGGGAGTCCCGTCACC CTCAGTTGTCAGGGGAGCCTTGAAGCCCAGGAGTACCGTCTATATAGGGAGAAAAAATCAGCATCTTGG ATTACACGGATACGACCAGAGCTTGTGAAGAACGGCCAGTTCCACATCCCATCCATCACCTGGGAACAC ACAGGGCGATATGGCTGTCAGTATTACAGCCGCGCTCGGTGGTCTGAGCTCAGTGACCCCCTGGTGCTG GTGATGACAGGAGCCTACCCAAAACCCACCCTCTCAGCCCAGCCCAGCCCTGTGGTGACCTCAGGAGGA AGGGTGACCCTCCAGTGTGAGTCACAGGTGGCATTTGGCGGCTTCATTCTGTGTAAGGAAGGAGAAGAT GAACACCCACAATGCCTGAACTCCCAGCCCCATGCCCGTGGGTCGTCCCGCGCCATCTTCTCCGTGGGC CCCGTGAGCCCGAATCGCAGGTGGTCGCACAGGTGCTATGGTTATGACTTGAACTCTCCCTATGTGTGG TCTTCACCCAGTGATCTCCTGGAGCTCCTGGTCCCAGGTGTTTCTAAGAAGCCATCACTCTCAGTGCAG CCGGGTCCTGTCATGGCCCCTGGGGAAAGCCTGACCCTCCAGTGTGTCTCTGATGTCGGCTATGACAGA TTTGTTCTGTACAAGGAGGGGGAACGTGACCTTCGCCAGCTCCCTGGCCGGCAGCCCCAGGCTGGGCTC TCCCAGGCCAACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTACGGTGCA CACAACCTCTCCTCTGAGTGCTCGGCCCCCAGCGACCCCCTGGACATCCTGATCACAGGACAGATCCGT GGCACACCCTTCATCTCAGTGCAGCCAGGCCCCACAGTGGCCTCAGGAGAGAACGTGACCCTGCTGTGT CAGTCATGGCGGCAGTTCCACACTTTCCTTCTGACCAAGGCGGGAGCAGCTGATGCCCCACTCCGTCTA AGATCAATACACGAATATCCTAAGTACCAGGCTGAATTCCCCATGAGTCCTGTGACCTCAGCCCACGCG GGGACCTACAGGTGCTACGGCTCACTCAACTCCGACCCCTACCTGCTGTCTCACCCCAGTGAGCCCCTG GAGCTCGTGGTCTCAGGACCCTCCATGGGTTCCAGCCCCCCACCCACCGGTCCCATCTCCACACCTGCA GGCCCTGAGGACCAGCCCCTCACCCCCACTGGGTCGGATCCCCAAAGTGGTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278406
Insert Size	1368 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278406.2
RefSeq Size	1422 bp
RefSeq ORF	1368 bp
Locus ID	10288
Protein Families	Transmembrane
MW	49.4 kDa
Gene Summary	This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (6) has a shorter 5' UTR, lacks several exons, and its 3'-terminal exon extends past a splice site that is used in variant 1. The resulting protein (isoform 5) has a shorter and distinct C-terminus, compared to isoform 1. Isoform 5 lacks the transmembrane domain found in isoform 1 and is suspected to be soluble (PMID: 19658091).

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